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P0CG08 (GPHRB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Golgi pH regulator B
Alternative name(s):
Protein GPR89B
Gene names
Name:GPR89B
Synonyms:GPHRB
ORF Names:HSPC201
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length455 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Voltage dependent anion channel required for acidification and functions of the Golgi apparatus that may function in counter-ion conductance By similarity. Ref.5

Subunit structure

Homotrimer By similarity. Ref.5

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein By similarity Ref.5.

Tissue specificity

Ubiquitous. Ref.5

Miscellaneous

Does not seem to be able to bind GTP.

Sequence similarities

Belongs to the Golgi pH regulator (TC 1.A.38) family. [View classification]

Sequence caution

The sequence AAF36121.1 differs from that shown. Reason: Erroneous termination at position 297. Translated as Cys.

Ontologies

Keywords
   Biological processIon transport
Protein transport
Transport
   Cellular componentGolgi apparatus
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionIon channel
Voltage-gated channel
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionvoltage-gated ion channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 455455Golgi pH regulator B
PRO_0000395006

Regions

Transmembrane5 – 2521Helical; Potential
Transmembrane46 – 6621Helical; Potential
Transmembrane79 – 9921Helical; Potential
Transmembrane114 – 13421Helical; Potential
Transmembrane150 – 17021Helical; Potential
Transmembrane290 – 31021Helical; Potential
Transmembrane343 – 36321Helical; Potential
Transmembrane378 – 39821Helical; Potential
Transmembrane425 – 44521Helical; Potential

Amino acid modifications

Glycosylation1801N-linked (GlcNAc...) Potential
Glycosylation2431N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict244 – 2474LTLI → NTMA in CAH56393. Ref.3
Sequence conflict2511V → E in AAF36121. Ref.4
Sequence conflict4181Q → R in AAF36121. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P0CG08 [UniParc].

Last modified June 15, 2010. Version 1.
Checksum: D831F66E682643F9

FASTA45552,917
        10         20         30         40         50         60 
MSFLIDSSIM ITSQILFFGF GWLFFMRQLF KDYEIRQYVV QVIFSVTFAF SCTMFELIIF 

        70         80         90        100        110        120 
EILGVLNSSS RYFHWKMNLC VILLILVFMV PFYIGYFIVS NIRLLHKQRL LFSCLLWLTF 

       130        140        150        160        170        180 
MYFFWKLGDP FPILSPKHGI LSIEQLISRV GVIGVTLMAL LSGFGAVNCP YTYMSYFLRN 

       190        200        210        220        230        240 
VTDTDILALE RRLLQTMDMI ISKKKRMAMA RRTMFQKGEV HNKPSGFWGM IKSVTTSASG 

       250        260        270        280        290        300 
SENLTLIQQE VDALEELSRQ LFLETADLYA TKERIEYSKT FKGKYFNFLG YFFSIYCVWK 

       310        320        330        340        350        360 
IFMATINIVF DRVGKTDPVT RGIEITVNYL GIQFDVKFWS QHISFILVGI IIVTSIRGLL 

       370        380        390        400        410        420 
ITLTKFFYAI SSSKSSNVIV LLLAQIMGMY FVSSVLLIRM SMPLEYRTII TEVLGELQFN 

       430        440        450 
FYHRWFDVIF LVSALSSILF LYLAHKQAPE KQMAP 

« Hide

References

« Hide 'large scale' references
[1]"Identification of 15 genes mapping to chromosome 6p21.3 spanning the microsatellite markers D6S306 and D6S1260. Characterization of three genes encoding zinc finger proteins."
Lee P.L., Gelbart T., West C., Adams M., Blackstone R., Meyer A.
Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Ovary.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-247.
Tissue: Testis.
[4]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 249-455.
Tissue: Umbilical cord blood.
[5]"GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus."
Maeda Y., Ide T., Koike M., Uchiyama Y., Kinoshita T.
Nat. Cell Biol. 10:1135-1145(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, TISSUE SPECIFICITY.
[6]"Two novel GPCR-type G proteins are abscisic acid receptors in Arabidopsis."
Pandey S., Nelson D.C., Assmann S.M.
Cell 136:136-148(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: LACK OF GTP-BINDING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U78723 mRNA. Translation: AAF21463.1.
AL445591, BX537254 Genomic DNA. Translation: CAH72386.1.
BX537254, AL445591 Genomic DNA. Translation: CAI18821.1.
AL133020 mRNA. Translation: CAH56393.1.
AF151035 mRNA. Translation: AAF36121.1. Sequence problems.
RefSeqNP_001091081.1. NM_001097612.1.
NP_057418.1. NM_016334.3.
XP_005277458.1. XM_005277401.2.
UniGeneHs.504320.
Hs.559115.
Hs.645432.

3D structure databases

ProteinModelPortalP0CG08.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119554. 1 interaction.
575849. 1 interaction.

Polymorphism databases

DMDM298351693.

Proteomic databases

PRIDEP0CG08.

Protocols and materials databases

DNASU51463.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000314163; ENSP00000358233; ENSG00000188092.
ENST00000580001; ENSP00000463624; ENSG00000264776.
GeneID51463.
653519.
KEGGhsa:51463.
hsa:653519.

Organism-specific databases

CTD51463.
653519.
GeneCardsGC01P147400.
H-InvDBHIX0000982.
HIX0056760.
HIX0159660.
HGNCHGNC:13840. GPR89B.
MIM612806. gene.
neXtProtNX_P0CG08.
PharmGKBPA134986137.
GenAtlasSearch...

Phylogenomic databases

OrthoDBEOG7HHWRV.
PhylomeDBP0CG08.
TreeFamTF313484.

Gene expression databases

BgeeP0CG08.

Family and domain databases

InterProIPR025969. ABA_GPCR_dom.
IPR022535. Golgi_pH-regulator_cons_dom.
IPR015672. GPCR89-rel.
[Graphical view]
PANTHERPTHR15948. PTHR15948. 1 hit.
PfamPF12430. ABA_GPCR. 1 hit.
PF12537. DUF3735. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio55087.
PROP0CG08.
SOURCESearch...

Entry information

Entry nameGPHRB_HUMAN
AccessionPrimary (citable) accession number: P0CG08
Secondary accession number(s): A6NN37 expand/collapse secondary AC list , B2RUV3, B3KMN3, Q53FQ9, Q5T2V8, Q5T5P5, Q659E2, Q6NVY5, Q9P0S4, Q9Y302
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: June 15, 2010
Last modified: April 16, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM