P0CG08 (GPHRB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgi pH regulator B

Alternative name(s):
Protein GPR89B

Gene names

Name:	GPR89B
Synonyms:	GPHRB
ORF Names:	HSPC201

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Voltage dependent anion channel required for acidification and functions of the Golgi apparatus that may function in counter-ion conductance By similarity. Ref.5
Subunit structure	Homotrimer By similarity. Ref.5
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein By similarity Ref.5.
Tissue specificity	Ubiquitous. Ref.5
Miscellaneous	Does not seem to be able to bind GTP.
Sequence similarities	Belongs to the Golgi pH regulator (TC 1.A.38) family. [View classification]
Sequence caution	The sequence AAF36121.1 differs from that shown. Reason: Erroneous termination at position 297. Translated as Cys.

Ontologies

Keywords
Biological process	Ion transport Protein transport Transport
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel Voltage-gated channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	voltage-gated ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	Golgi pH regulator B		PRO_0000395006
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	46 – 66	21	Helical; Potential
	Transmembrane	79 – 99	21	Helical; Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Transmembrane	290 – 310	21	Helical; Potential
	Transmembrane	343 – 363	21	Helical; Potential
	Transmembrane	378 – 398	21	Helical; Potential
	Transmembrane	425 – 445	21	Helical; Potential
Amino acid modifications
	Glycosylation	180	1	N-linked (GlcNAc...) Potential
	Glycosylation	243	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	244 – 247	4	LTLI → NTMA in CAH56393. Ref.3
	Sequence conflict	251	1	V → E in AAF36121. Ref.4
	Sequence conflict	418	1	Q → R in AAF36121. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P0CG08 [UniParc]. Last modified June 15, 2010. Version 1. Checksum: D831F66E682643F9	FASTA	455	52,917
	10 20 30 40 50 60 MSFLIDSSIM ITSQILFFGF GWLFFMRQLF KDYEIRQYVV QVIFSVTFAF SCTMFELIIF 70 80 90 100 110 120 EILGVLNSSS RYFHWKMNLC VILLILVFMV PFYIGYFIVS NIRLLHKQRL LFSCLLWLTF 130 140 150 160 170 180 MYFFWKLGDP FPILSPKHGI LSIEQLISRV GVIGVTLMAL LSGFGAVNCP YTYMSYFLRN 190 200 210 220 230 240 VTDTDILALE RRLLQTMDMI ISKKKRMAMA RRTMFQKGEV HNKPSGFWGM IKSVTTSASG 250 260 270 280 290 300 SENLTLIQQE VDALEELSRQ LFLETADLYA TKERIEYSKT FKGKYFNFLG YFFSIYCVWK 310 320 330 340 350 360 IFMATINIVF DRVGKTDPVT RGIEITVNYL GIQFDVKFWS QHISFILVGI IIVTSIRGLL 370 380 390 400 410 420 ITLTKFFYAI SSSKSSNVIV LLLAQIMGMY FVSSVLLIRM SMPLEYRTII TEVLGELQFN 430 440 450 FYHRWFDVIF LVSALSSILF LYLAHKQAPE KQMAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of 15 genes mapping to chromosome 6p21.3 spanning the microsatellite markers D6S306 and D6S1260. Characterization of three genes encoding zinc finger proteins." Lee P.L., Gelbart T., West C., Adams M., Blackstone R., Meyer A. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-247. Tissue: Testis.
[4]	"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. , Gu J., Chen S.-J., Chen Z. Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 249-455. Tissue: Umbilical cord blood.
[5]	"GPHR is a novel anion channel critical for acidification and functions of the Golgi apparatus." Maeda Y., Ide T., Koike M., Uchiyama Y., Kinoshita T. Nat. Cell Biol. 10:1135-1145(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, TISSUE SPECIFICITY.
[6]	"Two novel GPCR-type G proteins are abscisic acid receptors in Arabidopsis." Pandey S., Nelson D.C., Assmann S.M. Cell 136:136-148(2009) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF GTP-BINDING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U78723 mRNA. Translation: AAF21463.1. AL445591, BX537254 Genomic DNA. Translation: CAH72386.1. BX537254, AL445591 Genomic DNA. Translation: CAI18821.1. AL133020 mRNA. Translation: CAH56393.1. AF151035 mRNA. Translation: AAF36121.1. Sequence problems.
IPI	IPI00472858.
RefSeq	NP_001091081.1. NM_001097612.1. NP_057418.1. NM_016334.3.
UniGene	Hs.504320. Hs.559115. Hs.645432.
3D structure databases
ProteinModelPortal	P0CG08.
ModBase	Search...
Proteomic databases
PRIDE	P0CG08.
Protocols and materials databases
DNASU	51463.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000314163; ENSP00000358233; ENSG00000188092. ENST00000580001; ENSP00000463624; ENSG00000264776.
GeneID	51463. 653519.
KEGG	hsa:51463. hsa:653519.
Organism-specific databases
CTD	51463. 653519.
GeneCards	GC01P147400.
H-InvDB	HIX0000982. HIX0056760. HIX0159660.
HGNC	HGNC:13840. GPR89B.
MIM	612806. gene.
neXtProt	NX_P0CG08.
PharmGKB	PA134986137.
GenAtlas	Search...
Phylogenomic databases
PhylomeDB	P0CG08.
Gene expression databases
ArrayExpress	P0CG08.
Bgee	P0CG08.
GermOnline	ENSG00000188092. Homo sapiens.
Family and domain databases
InterPro	IPR025969. ABA_GPCR_dom. IPR022535. Golgi_pH-regulator_cons_dom. IPR015672. GPCR89-rel. [Graphical view]
PANTHER	PTHR15948. PTHR15948. 1 hit.
Pfam	PF12430. ABA_GPCR. 1 hit. PF12537. DUF3735. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	55087.
SOURCE	Search...

Entry information

Entry name

GPHRB_HUMAN

Accession

Primary (citable) accession number: P0CG08
Secondary accession number(s): A6NN37 , B2RUV3, B3KMN3, Q53FQ9, Q5T2V8, Q5T5P5, Q659E2, Q6NVY5, Q9P0S4, Q9Y302

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	April 3, 2013
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.