P0CG07 (SCRA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Defensin-like protein A
Short name=AtSCRA

Alternative name(s):
S locus cysteine-rich-like protein A
S locus protein 11

Gene names

Name:	SCRA
Synonyms:	SCR1, SP11
Ordered Locus Names:	At4g21364
ORF Names:	T6K22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	62 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Truncated and inactivated form of SCRA, a protein involved in male-mediated self-incompatibility when active. Most A.thaliana cultivars contain such an inactive form and thus, are self-fertiles. Ref.1 Ref.3 Ref.4 Ref.5
Subcellular location	Secreted By similarity.
Miscellaneous	By contrast to A.lyrata and A.halleri, self-incompatible species with full-length SCRA isoforms, A.thaliana is self-compatible with impaired SCRA protein.
Sequence similarities	Belongs to the DEFL family.

Ontologies

Keywords
Biological process	Self-incompatibility
Cellular component	Secreted
Domain	Signal
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rejection of self pollen Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 62	36	Defensin-like protein A		PRO_0000394667
Amino acid modifications
	Disulfide bond	42 ↔ 52		By similarity
Experimental info
	Mutagenesis	56 – 62	7	DAKKTLA → IAKNFRPSRPFECNCQTFDK GGICYCKKCLV: Confers male-driven self-incompatibility. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0CG07 [UniParc]. Last modified June 15, 2010. Version 1. Checksum: 863A76970BCF46A9	FASTA	62	7,226
	10 20 30 40 50 60 MRCVVLFMVS CLLIVLLINH FEEVEAQKWN KCFLRDIFPG KCEHDANAKL RCKEDDAKKT LA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene." Tsuchimatsu T., Suwabe K., Shimizu-Inatsugi R., Isokawa S., Pavlidis P., Stadler T., Suzuki G., Takayama S., Watanabe M., Shimizu K.K. Nature 464:1342-1346(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, MUTAGENESIS OF 56-ASP--ALA-62. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Natural variation in expression of self-incompatibility in Arabidopsis thaliana: implications for the evolution of selfing." Nasrallah M.E., Liu P., Sherman-Broyles S., Boggs N.A., Nasrallah J.B. Proc. Natl. Acad. Sci. U.S.A. 101:16070-16074(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"The transition to self-compatibility in Arabidopsis thaliana and evolution within S-haplotypes over 10 Myr." Bechsgaard J.S., Castric V., Charlesworth D., Vekemans X., Schierup M.H. Mol. Biol. Evol. 23:1741-1750(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Independent S-locus mutations caused self-fertility in Arabidopsis thaliana." Boggs N.A., Nasrallah J.B., Nasrallah M.E. PLoS Genet. 5:E1000426-E1000426(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Web resources

Protein Spotlight

Do it yourself - Issue 128 of May 2011

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL031187 Genomic DNA. No translation available.
IPI	IPI00968556.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
TAIR	At4g21364.
Family and domain databases
InterPro	IPR010682. SCRL. [Graphical view]
Pfam	PF06876. SCRL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRA_ARATH

Accession

Primary (citable) accession number: P0CG07

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	April 3, 2013
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program