P0CF92 (INSL2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative transposase InsL for insertion sequence element IS186B

Gene names

Name:	insL2
Ordered Locus Names:	b0582, JW0572

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the transposition of the insertion sequence IS186.
Sequence similarities	Belongs to the transposase 11 family.
Sequence caution	The sequence AAB40781.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	DNA recombination Transposition
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome Transposable element
Gene Ontology (GO)
Biological_process	transposition, DNA-mediated Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transposase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Putative transposase InsL for insertion sequence element IS186B		PRO_0000394137

Sequences

	Sequence		Length	Mass (Da)
	P0CF92 [UniParc]. Last modified May 18, 2010. Version 1. Checksum: FDD2041F5F3D7F03	FASTA	370	40,909
	10 20 30 40 50 60 MNYSHDNWSA ILAHIGKPEE LDTSARNAGA LTRRREIRDA ATLLRLGLAY GPGGMSLREV 70 80 90 100 110 120 TAWAQLHDVA TLSDVALLKR LRNAADWFGI LAAQTLAVRA AVTGCTSGKR LRLVDGTAIS 130 140 150 160 170 180 APGGGSAEWR LHMGYDPHTC QFTDFELTDS RDAERLDRFA QTADEIRIAD RGFGSRPECI 190 200 210 220 230 240 RSLAFGEADY IVRVHWRGLR WLTAEGMRFD MMGFLRGLDC GKNGETTVMI GNSGNKKAGA 250 260 270 280 290 300 PFPARLIAVS LPPEKALISK TRLLSENRRK GRVVQAETLE AAGHVLLLTS LPEDEYSAEQ 310 320 330 340 350 360 VADCYRLRWQ IELAFKRLKS LLHLDALRAK EPELAKAWIF ANLLAAFLID DIIQPSLDFP 370 PRSAGSEKKN

References

[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82598 Genomic DNA. Translation: AAB40781.1. Different initiation. U00096 Genomic DNA. Translation: AAC73683.1. AP009048 Genomic DNA. Translation: BAA35223.1.
PIR	QQEC47. G65013.
RefSeq	NP_414557.1. NC_000913.2. NP_415114.1. NC_000913.2. NP_416895.2. NC_000913.2. YP_488322.1. NC_007779.1. YP_488870.1. NC_007779.1. YP_490635.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0CF92.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73683; AAC73683; b0582. BAA35223; BAA35223; BAA35223.
GeneID	12930191. 12930725. 12931716. 944754. 945200. 946896.
KEGG	ecj:Y75_p0016. ecj:Y75_p0570. ecj:Y75_p2360. eco:b0016. eco:b0582. eco:b2394.
Organism-specific databases
EchoBASE	EB4754.
EcoGene	EG40012. insL2.
Enzyme and pathway databases
BioCyc	EcoCyc:MONOMER0-4232.
Family and domain databases
Gene3D	3.90.350.10. 1 hit.
InterPro	IPR012337. RNaseH-like_dom. IPR002559. Transposase_11. IPR014736. Transposase_Tn5-like_core. [Graphical view]
Pfam	PF01609. DDE_Tnp_1. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

INSL2_ECOLI

Accession

Primary (citable) accession number: P0CF92
Secondary accession number(s): P08409 , P11307, P76952, P77426, Q2MCH6, Q47051

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 18, 2010
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program