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Protein

Transposase InsI for insertion sequence element IS30D

Gene

insI4

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Required for the transposition of the insertion element.Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA recombination, Transposition

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-4233.

Names & Taxonomyi

Protein namesi
Recommended name:
Transposase InsI for insertion sequence element IS30D
Gene namesi
Name:insI4
Ordered Locus Names:b4284, JW4244
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG40009. insI4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Transposase InsI for insertion sequence element IS30DPRO_0000394136Add
BLAST

Proteomic databases

EPDiP0CF90.
PaxDbiP0CF90.
PRIDEiP0CF90.

Interactioni

Protein-protein interaction databases

STRINGi511145.b4284.

Structurei

3D structure databases

ProteinModelPortaliP0CF90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini213 – 379167Integrase catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the transposase IS30 family.Curated
Contains 1 integrase catalytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105F9F. Bacteria.
COG2826. LUCA.
HOGENOMiHOG000114767.
InParanoidiP0CF90.
PhylomeDBiP0CF90.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR001584. Integrase_cat-core.
IPR025246. IS30-like_HTH.
IPR012337. RNaseH-like_dom.
IPR001598. Transposase_IS30_CS.
[Graphical view]
PfamiPF13936. HTH_38. 1 hit.
PF00665. rve. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50994. INTEGRASE. 1 hit.
PS01043. TRANSPOSASE_IS30. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CF90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRTFTAEEK ASVFELWKNG TGFSEIANIL GSKPGTIFTM LRDTGGIKPH
60 70 80 90 100
ERKRAVAHLT LSEREEIRAG LSAKMSIRAI ATALNRSPST ISREVQRNRG
110 120 130 140 150
RRYYKAVDAN NRANRMAKRP KPCLLDQNLP LRKLVLEKLE MKWSPEQISG
160 170 180 190 200
WLRRTKPRQK TLRISPETIY KTLYFRSREA LHHLNIQHLR RSHSLRHGRR
210 220 230 240 250
HTRKGERGTI NIVNGTPIHE RSRNIDNRRS LGHWEGDLVS GTKNSHIATL
260 270 280 290 300
VDRKSRYTII LRLRGKDSVS VNQALTDKFL SLPSELRKSL TWDRGMELAR
310 320 330 340 350
HLEFTVSTGV KVYFCDPQSP WQRGTNENTN GLIRQYFPKK TCLAQYTQHE
360 370 380
LDLVAAQLNN RPRKTLKFKT PKEIIERGVA LTD
Length:383
Mass (Da):44,281
Last modified:May 18, 2010 - v1
Checksum:i6AADF2CD604B0F83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97180.1.
U00096 Genomic DNA. Translation: AAC77240.1.
AP009048 Genomic DNA. Translation: BAE78276.1.
PIRiF65241.
RefSeqiNP_415922.1. NC_000913.3.
NP_418704.1. NC_000913.3.
WP_001254932.1. NZ_LN832404.1.
YP_001294740.1. NC_009602.1.

Genome annotation databases

EnsemblBacteriaiAAC77240; AAC77240; b4284.
BAE78276; BAE78276; BAE78276.
GeneIDi5290948.
945968.
948815.
KEGGiecj:JW4244.
eco:b1404.
eco:b4284.
pg:5290948.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97180.1.
U00096 Genomic DNA. Translation: AAC77240.1.
AP009048 Genomic DNA. Translation: BAE78276.1.
PIRiF65241.
RefSeqiNP_415922.1. NC_000913.3.
NP_418704.1. NC_000913.3.
WP_001254932.1. NZ_LN832404.1.
YP_001294740.1. NC_009602.1.

3D structure databases

ProteinModelPortaliP0CF90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b4284.

Proteomic databases

EPDiP0CF90.
PaxDbiP0CF90.
PRIDEiP0CF90.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77240; AAC77240; b4284.
BAE78276; BAE78276; BAE78276.
GeneIDi5290948.
945968.
948815.
KEGGiecj:JW4244.
eco:b1404.
eco:b4284.
pg:5290948.

Organism-specific databases

EchoBASEiEB4726.
EcoGeneiEG40009. insI4.

Phylogenomic databases

eggNOGiENOG4105F9F. Bacteria.
COG2826. LUCA.
HOGENOMiHOG000114767.
InParanoidiP0CF90.
PhylomeDBiP0CF90.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-4233.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR001584. Integrase_cat-core.
IPR025246. IS30-like_HTH.
IPR012337. RNaseH-like_dom.
IPR001598. Transposase_IS30_CS.
[Graphical view]
PfamiPF13936. HTH_38. 1 hit.
PF00665. rve. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50994. INTEGRASE. 1 hit.
PS01043. TRANSPOSASE_IS30. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiINSI4_ECOLI
AccessioniPrimary (citable) accession number: P0CF90
Secondary accession number(s): P37246
, P77341, Q2M630, Q2MCF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: May 18, 2010
Last modified: March 16, 2016
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome, Transposable element

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.