Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0CF35

- FDH2_YEAST

UniProt

P0CF35 - FDH2_YEAST

Protein

Putative formate dehydrogenase 2

Gene

FDH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein uncertaini
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 31 (01 Oct 2014)
      Sequence version 1 (20 Apr 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Formate + NAD+ = CO2 + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei132 – 1321By similarity
    Binding sitei156 – 1561NADBy similarity
    Active sitei185 – 1851Proton donorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi36 – 372NADBy similarity
    Nucleotide bindingi130 – 1323NADBy similarity
    Nucleotide bindingi185 – 1884NADBy similarity

    GO - Molecular functioni

    1. cofactor binding Source: InterPro
    2. formate dehydrogenase (NAD+) activity Source: SGD
    3. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro

    GO - Biological processi

    1. formate catabolic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative formate dehydrogenase 2 (EC:1.2.1.2)
    Alternative name(s):
    NAD-dependent formate dehydrogenase 2
    Gene namesi
    Name:FDH2
    Ordered Locus Names:YPL275W
    ORF Names:P0326
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    SGDiS000006196. FDH2.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosol Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 236236Putative formate dehydrogenase 2PRO_0000223648Add
    BLAST

    Expressioni

    Inductioni

    Induced by formate.

    Gene expression databases

    GenevestigatoriQ08987.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CF35.
    SMRiP0CF35. Positions 3-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    OrthoDBiEOG7MKWHQ.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006140. D-isomer_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF02826. 2-Hacid_dh_C. 1 hit.
    [Graphical view]
    PROSITEiPS00670. D_2_HYDROXYACID_DH_2. 1 hit.
    PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0CF35-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVVINKQLMV SGILPAWLKN EYDLEDKIIS TVGAGRIGYR VLERLVAFNP    50
    KKLLYYDYQE LPAEAINRLN EASKLFNGRG DIVQRVEKLE DMVAQSDVVT 100
    INCPLHKDSR GLFNKKLISH MKDGAYLVNT ARGAICVAED VAEAVKSGKL 150
    AGYGGDVWDK QPAPKDHPWR TMDNKDHVGN AMTVHISGTS LHAQKRYAQG 200
    VKNILNSYFS KKFDYRPQDI IVQNGSYATR AYGQKK 236
    Length:236
    Mass (Da):26,487
    Last modified:April 20, 2010 - v1
    Checksum:i1C8E40684606D166
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73632 Genomic DNA. Translation: CAA98013.1.
    BK006949 Genomic DNA. No translation available.
    PIRiS65308.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73632 Genomic DNA. Translation: CAA98013.1 .
    BK006949 Genomic DNA. No translation available.
    PIRi S65308.

    3D structure databases

    ProteinModelPortali P0CF35.
    SMRi P0CF35. Positions 3-227.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    SGDi S000006196. FDH2.

    Phylogenomic databases

    OrthoDBi EOG7MKWHQ.

    Gene expression databases

    Genevestigatori Q08987.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006140. D-isomer_DH_NAD-bd.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF02826. 2-Hacid_dh_C. 1 hit.
    [Graphical view ]
    PROSITEi PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
    PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Functional analysis of structural genes for NAD(+)-dependent formate dehydrogenase in Saccharomyces cerevisiae."
      Overkamp K.M., Koetter P., van der Hoek R., Schoondermark-Stolk S., Luttik M.A.H., van Dijken J.P., Pronk J.T.
      Yeast 19:509-520(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-64, CONFIRMATION OF STOP CODON.
      Strain: ATCC 200060 / W303 and ATCC 201388 / BY4741.

    Entry informationi

    Entry nameiFDH2_YEAST
    AccessioniPrimary (citable) accession number: P0CF35
    Secondary accession number(s): Q08987, Q08988
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 20, 2010
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 31 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Caution

    Could be the product of a pseudogene. This is the C-terminal part of a second copy of formate dehydrogenase in the yeast genome. Strains BY4741, S288c and W303 have a stop codon in position 146, which disrupts the gene coding for this protein and produces two ORFs YPL275W and YPL276W. A contiguous sequence for formate dehydrogenase 2 can be found in strain CEN.PK113-7D (AC P0CT22).Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3