Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0CF32

- SDC25_YEASX

UniProt

P0CF32 - SDC25_YEASX

Protein

Guanine nucleotide exchange factor SDC25

Gene

SDC25

Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 25 (01 Oct 2014)
      Sequence version 1 (20 Apr 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Promotes the exchange of Ras-bound GDP by GTP.3 Publications

    GO - Molecular functioni

    1. guanyl-nucleotide exchange factor activity Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. regulation of small GTPase mediated signal transduction Source: InterPro
    4. small GTPase mediated signal transduction Source: InterPro

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Biological processi

    Cell cycle, Cell division

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide exchange factor SDC25
    Gene namesi
    Name:SDC25
    Synonyms:SCD25
    OrganismiSaccharomyces cerevisiae (Baker's yeast)
    Taxonomic identifieri4932 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12531253Guanine nucleotide exchange factor SDC25PRO_0000068893Add
    BLAST

    Proteomic databases

    PeptideAtlasiP14771.

    Expressioni

    Gene expression databases

    GenevestigatoriP14771.

    Interactioni

    Protein-protein interaction databases

    IntActiP0CF32. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CF32.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 9772SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini782 – 914133N-terminal Ras-GEFPROSITE-ProRule annotationAdd
    BLAST
    Domaini952 – 1199248Ras-GEFPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
    Contains 1 Ras-GEF domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Family and domain databases

    Gene3Di1.10.840.10. 1 hit.
    InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
    IPR019804. Ras_G-nucl-exch_fac_CS.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    SMARTiSM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48366. SSF48366. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEiPS00720. RASGEF. 1 hit.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0CF32-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCTASYAGM TTPVKDKEGH GIPCLQPIDV VECTYQYFTK SRNKLSLRVG     50
    DLIYVLTKGS NGWWDGVLIR HSANNNNNNS LILDRGWFPP SFTRSILNEL 100
    HGVPDIGNEL EIFQAGLNLK LELSSNPVIL SLEDFLDCCR DIEFKEQLAW 150
    SPTPVHERKG CCELLYYNQD LDVYCRTLPY LPQNQVETVN DYSSFPAISK 200
    IAGKKMPITS SPDLFYLNDC DVVYWYDLTR LVCHYVNLTE RDLLANEREK 250
    FLTSLDLLTA QITYVYMLFR NLRLVEDSFK KTLKKLIYTL SRFSINANIW 300
    FHSTSFEERE AIASQKDPER RSPLLQSILG TFQKFHFLLR LLHFLSNPNE 350
    LTILPQLTPR FFKDSFNTIS WNNPFLRTVF NQHMSMTLPR QMIKAVAGAS 400
    GIVAENIDEI PASKQGTFIS SETSHHSPSA PFQRRRRGTI FSNVSGSSDE 450
    SDTIWSKRKK PYPLNEETLS LVRARKKQLD GKLKQMIKSA NEYLSNTANF 500
    SKMLNFEMNF KTYEEVSGTI PIIDILENLD LTIFLNLREL GDENRVFDED 550
    VAIGDEDKEF LKHSLSSLSY ILSDYFNMKQ YFHDVVVKFI IVAQHLTLED 600
    PFVFSPMQND LPTGYYEPMK PSSLNLDNAK DKKNGSQNTD IQEEEDEYEP 650
    DPDSLILFHN LINQDSDFND LKFFNLAHVF KKSCDDYFDV LKLAIEFVNQ 700
    LILERENLLN YAARMMKNNI TELLLRGEEG YGSYDGGETA EKSDTNAVYA 750
    DSDTKDNDEW RDSQVKLPRY LQREYDSELI WGPIVRIKGG SKHALISYLT 800
    DNEKKDLFFN ITFLITFRSI FTTTEFLSYL ISQYNLDPPE DLCFEEYNEW 850
    VTKKLIPVKC RVVEIMTTFF KQYWFPGYDE PDLATLNLDY FAQVAIKENI 900
    TGSVELLKEV NQKFKLGNIQ EATAPMKTLD QQICQDHYSG TLYSTTESIL 950
    AVDPVLFATQ LTILEHEIYC EITIFDCLQK IWKNKYTKSY GASPGLNEFI 1000
    SFANKLTNFI SYSVVKEADK SKRAKLLSHF IFIAEYCRKF NNFSSMTAII 1050
    SALYSSPIYR LEKTWQAVIP QTRDLLQSLN KLMDPKKNFI NYRNELKSLH 1100
    SAPCVPFFGV YLSDLTFTDS GNPDYLVLEH GLKGVHDEKK YINFNKRSRL 1150
    VDILQEIIYF KKTHYDFTKD RTVIECISNS LENIPHIEKQ YQLSLIIEPK 1200
    PRKKVVPNSN SNNKSQEKSR DDQTDEGKTS TKKDRFPKFQ LHKTKKKAPK 1250
    VSK 1253
    Length:1,253
    Mass (Da):144,980
    Last modified:April 20, 2010 - v1
    Checksum:i2DE2C9EC27E3E60D
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti682 – 69211KSCDDYFDVLK → NPVMTILMCLN in strain: 0L136.
    Add
    BLAST
    Natural varianti740 – 7401Missing in strain: 0L136.
    Natural varianti783 – 7853PIV → SNN in strain: 0L136.
    Natural varianti974 – 9741I → T in strain: 0L136.
    Natural varianti1048 – 10481A → D in strain: 0L136.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26647 Genomic DNA. Translation: AAA16565.1.
    PIRiS14177.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26647 Genomic DNA. Translation: AAA16565.1 .
    PIRi S14177.

    3D structure databases

    ProteinModelPortali P0CF32.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0CF32. 4 interactions.

    Proteomic databases

    PeptideAtlasi P14771.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Gene expression databases

    Genevestigatori P14771.

    Family and domain databases

    Gene3Di 1.10.840.10. 1 hit.
    InterProi IPR000651. Ras-like_Gua-exchang_fac_N.
    IPR019804. Ras_G-nucl-exch_fac_CS.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    SMARTi SM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48366. SSF48366. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEi PS00720. RASGEF. 1 hit.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SDC25, a CDC25-like gene which contains a RAS-activating domain and is a dispensable gene of Saccharomyces cerevisiae."
      Damak F., Boy-Marcotte E., le Roscouet D., Guilbaud R., Jacquet M.
      Mol. Cell. Biol. 11:202-212(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 200060 / W303.
    2. "The C-terminal part of a gene partially homologous to CDC 25 gene suppresses the cdc25-5 mutation in Saccharomyces cerevisiae."
      Boy-Marcotte E., Damak F., Camonis J., Garreau H., Jacquet M.
      Gene 77:21-30(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 670-1253.
      Strain: 0L136.
    3. "Enhancement of the GDP-GTP exchange of RAS proteins by the carboxyl-terminal domain of SCD25."
      Crechet J.B., Poullet P., Mistou M.-Y., Parmeggiani A., Camonis J., Boy-Marcotte E., Damak F., Jacquet M.
      Science 248:866-868(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "The COOH-domain of the product of the Saccharomyces cerevisiae SCD25 gene elicits activation of p21-ras proteins in mammalian cells."
      Rey I., Schweighoffer F., Barlat I., Camonis J., Boy-Marcotte E., Guilbaud R., Jacquet M., Tocque B.
      Oncogene 6:347-349(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "SDC25, a dispensable Ras guanine nucleotide exchange factor of Saccharomyces cerevisiae differs from CDC25 by its regulation."
      Boy-Marcotte E., Ikonomi P., Jacquet M.
      Mol. Biol. Cell 7:529-539(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSDC25_YEASX
    AccessioniPrimary (citable) accession number: P0CF32
    Secondary accession number(s): P14771
    , Q12037, Q12065, Q6B0T5, Q6WV04
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 20, 2010
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Suppresses the CDC25-5 mutation in yeast (restores cAMP level) and has similar functions as CDC25.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3