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P0CF32

- SDC25_YEASX

UniProt

P0CF32 - SDC25_YEASX

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Protein

Guanine nucleotide exchange factor SDC25

Gene
SDC25, SCD25
Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Promotes the exchange of Ras-bound GDP by GTP.3 Publications

GO - Molecular functioni

  1. guanyl-nucleotide exchange factor activity Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. regulation of small GTPase mediated signal transduction Source: InterPro
  4. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Cell cycle, Cell division

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor SDC25
Gene namesi
Name:SDC25
Synonyms:SCD25
OrganismiSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifieri4932 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12531253Guanine nucleotide exchange factor SDC25PRO_0000068893Add
BLAST

Proteomic databases

PeptideAtlasiP14771.

Expressioni

Gene expression databases

GenevestigatoriP14771.

Interactioni

Protein-protein interaction databases

IntActiP0CF32. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliP0CF32.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 9772SH3Add
BLAST
Domaini782 – 914133N-terminal Ras-GEFAdd
BLAST
Domaini952 – 1199248Ras-GEFAdd
BLAST

Sequence similaritiesi

Contains 1 Ras-GEF domain.
Contains 1 SH3 domain.

Keywords - Domaini

SH3 domain

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CF32-1 [UniParc]FASTAAdd to Basket

« Hide

MSCTASYAGM TTPVKDKEGH GIPCLQPIDV VECTYQYFTK SRNKLSLRVG     50
DLIYVLTKGS NGWWDGVLIR HSANNNNNNS LILDRGWFPP SFTRSILNEL 100
HGVPDIGNEL EIFQAGLNLK LELSSNPVIL SLEDFLDCCR DIEFKEQLAW 150
SPTPVHERKG CCELLYYNQD LDVYCRTLPY LPQNQVETVN DYSSFPAISK 200
IAGKKMPITS SPDLFYLNDC DVVYWYDLTR LVCHYVNLTE RDLLANEREK 250
FLTSLDLLTA QITYVYMLFR NLRLVEDSFK KTLKKLIYTL SRFSINANIW 300
FHSTSFEERE AIASQKDPER RSPLLQSILG TFQKFHFLLR LLHFLSNPNE 350
LTILPQLTPR FFKDSFNTIS WNNPFLRTVF NQHMSMTLPR QMIKAVAGAS 400
GIVAENIDEI PASKQGTFIS SETSHHSPSA PFQRRRRGTI FSNVSGSSDE 450
SDTIWSKRKK PYPLNEETLS LVRARKKQLD GKLKQMIKSA NEYLSNTANF 500
SKMLNFEMNF KTYEEVSGTI PIIDILENLD LTIFLNLREL GDENRVFDED 550
VAIGDEDKEF LKHSLSSLSY ILSDYFNMKQ YFHDVVVKFI IVAQHLTLED 600
PFVFSPMQND LPTGYYEPMK PSSLNLDNAK DKKNGSQNTD IQEEEDEYEP 650
DPDSLILFHN LINQDSDFND LKFFNLAHVF KKSCDDYFDV LKLAIEFVNQ 700
LILERENLLN YAARMMKNNI TELLLRGEEG YGSYDGGETA EKSDTNAVYA 750
DSDTKDNDEW RDSQVKLPRY LQREYDSELI WGPIVRIKGG SKHALISYLT 800
DNEKKDLFFN ITFLITFRSI FTTTEFLSYL ISQYNLDPPE DLCFEEYNEW 850
VTKKLIPVKC RVVEIMTTFF KQYWFPGYDE PDLATLNLDY FAQVAIKENI 900
TGSVELLKEV NQKFKLGNIQ EATAPMKTLD QQICQDHYSG TLYSTTESIL 950
AVDPVLFATQ LTILEHEIYC EITIFDCLQK IWKNKYTKSY GASPGLNEFI 1000
SFANKLTNFI SYSVVKEADK SKRAKLLSHF IFIAEYCRKF NNFSSMTAII 1050
SALYSSPIYR LEKTWQAVIP QTRDLLQSLN KLMDPKKNFI NYRNELKSLH 1100
SAPCVPFFGV YLSDLTFTDS GNPDYLVLEH GLKGVHDEKK YINFNKRSRL 1150
VDILQEIIYF KKTHYDFTKD RTVIECISNS LENIPHIEKQ YQLSLIIEPK 1200
PRKKVVPNSN SNNKSQEKSR DDQTDEGKTS TKKDRFPKFQ LHKTKKKAPK 1250
VSK 1253
Length:1,253
Mass (Da):144,980
Last modified:April 20, 2010 - v1
Checksum:i2DE2C9EC27E3E60D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti682 – 69211KSCDDYFDVLK → NPVMTILMCLN in strain: 0L136.
Add
BLAST
Natural varianti740 – 7401Missing in strain: 0L136.
Natural varianti783 – 7853PIV → SNN in strain: 0L136.
Natural varianti974 – 9741I → T in strain: 0L136.
Natural varianti1048 – 10481A → D in strain: 0L136.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26647 Genomic DNA. Translation: AAA16565.1.
PIRiS14177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26647 Genomic DNA. Translation: AAA16565.1 .
PIRi S14177.

3D structure databases

ProteinModelPortali P0CF32.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P0CF32. 4 interactions.

Proteomic databases

PeptideAtlasi P14771.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Gene expression databases

Genevestigatori P14771.

Family and domain databases

Gene3Di 1.10.840.10. 1 hit.
InterProi IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
SMARTi SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF48366. SSF48366. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEi PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "SDC25, a CDC25-like gene which contains a RAS-activating domain and is a dispensable gene of Saccharomyces cerevisiae."
    Damak F., Boy-Marcotte E., le Roscouet D., Guilbaud R., Jacquet M.
    Mol. Cell. Biol. 11:202-212(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 200060 / W303.
  2. "The C-terminal part of a gene partially homologous to CDC 25 gene suppresses the cdc25-5 mutation in Saccharomyces cerevisiae."
    Boy-Marcotte E., Damak F., Camonis J., Garreau H., Jacquet M.
    Gene 77:21-30(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 670-1253.
    Strain: 0L136.
  3. "Enhancement of the GDP-GTP exchange of RAS proteins by the carboxyl-terminal domain of SCD25."
    Crechet J.B., Poullet P., Mistou M.-Y., Parmeggiani A., Camonis J., Boy-Marcotte E., Damak F., Jacquet M.
    Science 248:866-868(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "The COOH-domain of the product of the Saccharomyces cerevisiae SCD25 gene elicits activation of p21-ras proteins in mammalian cells."
    Rey I., Schweighoffer F., Barlat I., Camonis J., Boy-Marcotte E., Guilbaud R., Jacquet M., Tocque B.
    Oncogene 6:347-349(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "SDC25, a dispensable Ras guanine nucleotide exchange factor of Saccharomyces cerevisiae differs from CDC25 by its regulation."
    Boy-Marcotte E., Ikonomi P., Jacquet M.
    Mol. Biol. Cell 7:529-539(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSDC25_YEASX
AccessioniPrimary (citable) accession number: P0CF32
Secondary accession number(s): P14771
, Q12037, Q12065, Q6B0T5, Q6WV04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: June 11, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Suppresses the CDC25-5 mutation in yeast (restores cAMP level) and has similar functions as CDC25.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi