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Protein

Guanine nucleotide exchange factor SDC25

Gene

SDC25

Organism
Saccharomyces cerevisiae (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Promotes the exchange of Ras-bound GDP by GTP.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Cell cycle, Cell division

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor SDC25
Gene namesi
Name:SDC25
Synonyms:SCD25
OrganismiSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifieri4932 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12531253Guanine nucleotide exchange factor SDC25PRO_0000068893Add
BLAST

Proteomic databases

PeptideAtlasiP14771.

Interactioni

Protein-protein interaction databases

IntActiP0CF32. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliP0CF32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 9772SH3PROSITE-ProRule annotationAdd
BLAST
Domaini782 – 914133N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini952 – 1199248Ras-GEFPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CF32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCTASYAGM TTPVKDKEGH GIPCLQPIDV VECTYQYFTK SRNKLSLRVG
60 70 80 90 100
DLIYVLTKGS NGWWDGVLIR HSANNNNNNS LILDRGWFPP SFTRSILNEL
110 120 130 140 150
HGVPDIGNEL EIFQAGLNLK LELSSNPVIL SLEDFLDCCR DIEFKEQLAW
160 170 180 190 200
SPTPVHERKG CCELLYYNQD LDVYCRTLPY LPQNQVETVN DYSSFPAISK
210 220 230 240 250
IAGKKMPITS SPDLFYLNDC DVVYWYDLTR LVCHYVNLTE RDLLANEREK
260 270 280 290 300
FLTSLDLLTA QITYVYMLFR NLRLVEDSFK KTLKKLIYTL SRFSINANIW
310 320 330 340 350
FHSTSFEERE AIASQKDPER RSPLLQSILG TFQKFHFLLR LLHFLSNPNE
360 370 380 390 400
LTILPQLTPR FFKDSFNTIS WNNPFLRTVF NQHMSMTLPR QMIKAVAGAS
410 420 430 440 450
GIVAENIDEI PASKQGTFIS SETSHHSPSA PFQRRRRGTI FSNVSGSSDE
460 470 480 490 500
SDTIWSKRKK PYPLNEETLS LVRARKKQLD GKLKQMIKSA NEYLSNTANF
510 520 530 540 550
SKMLNFEMNF KTYEEVSGTI PIIDILENLD LTIFLNLREL GDENRVFDED
560 570 580 590 600
VAIGDEDKEF LKHSLSSLSY ILSDYFNMKQ YFHDVVVKFI IVAQHLTLED
610 620 630 640 650
PFVFSPMQND LPTGYYEPMK PSSLNLDNAK DKKNGSQNTD IQEEEDEYEP
660 670 680 690 700
DPDSLILFHN LINQDSDFND LKFFNLAHVF KKSCDDYFDV LKLAIEFVNQ
710 720 730 740 750
LILERENLLN YAARMMKNNI TELLLRGEEG YGSYDGGETA EKSDTNAVYA
760 770 780 790 800
DSDTKDNDEW RDSQVKLPRY LQREYDSELI WGPIVRIKGG SKHALISYLT
810 820 830 840 850
DNEKKDLFFN ITFLITFRSI FTTTEFLSYL ISQYNLDPPE DLCFEEYNEW
860 870 880 890 900
VTKKLIPVKC RVVEIMTTFF KQYWFPGYDE PDLATLNLDY FAQVAIKENI
910 920 930 940 950
TGSVELLKEV NQKFKLGNIQ EATAPMKTLD QQICQDHYSG TLYSTTESIL
960 970 980 990 1000
AVDPVLFATQ LTILEHEIYC EITIFDCLQK IWKNKYTKSY GASPGLNEFI
1010 1020 1030 1040 1050
SFANKLTNFI SYSVVKEADK SKRAKLLSHF IFIAEYCRKF NNFSSMTAII
1060 1070 1080 1090 1100
SALYSSPIYR LEKTWQAVIP QTRDLLQSLN KLMDPKKNFI NYRNELKSLH
1110 1120 1130 1140 1150
SAPCVPFFGV YLSDLTFTDS GNPDYLVLEH GLKGVHDEKK YINFNKRSRL
1160 1170 1180 1190 1200
VDILQEIIYF KKTHYDFTKD RTVIECISNS LENIPHIEKQ YQLSLIIEPK
1210 1220 1230 1240 1250
PRKKVVPNSN SNNKSQEKSR DDQTDEGKTS TKKDRFPKFQ LHKTKKKAPK

VSK
Length:1,253
Mass (Da):144,980
Last modified:April 20, 2010 - v1
Checksum:i2DE2C9EC27E3E60D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti682 – 69211KSCDDYFDVLK → NPVMTILMCLN in strain: 0L136.
Add
BLAST
Natural varianti740 – 7401Missing in strain: 0L136.
Natural varianti783 – 7853PIV → SNN in strain: 0L136.
Natural varianti974 – 9741I → T in strain: 0L136.
Natural varianti1048 – 10481A → D in strain: 0L136.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26647 Genomic DNA. Translation: AAA16565.1.
PIRiS14177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26647 Genomic DNA. Translation: AAA16565.1.
PIRiS14177.

3D structure databases

ProteinModelPortaliP0CF32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0CF32. 4 interactions.

Proteomic databases

PeptideAtlasiP14771.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
InterProiIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48366. SSF48366. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "SDC25, a CDC25-like gene which contains a RAS-activating domain and is a dispensable gene of Saccharomyces cerevisiae."
    Damak F., Boy-Marcotte E., le Roscouet D., Guilbaud R., Jacquet M.
    Mol. Cell. Biol. 11:202-212(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 200060 / W303.
  2. "The C-terminal part of a gene partially homologous to CDC 25 gene suppresses the cdc25-5 mutation in Saccharomyces cerevisiae."
    Boy-Marcotte E., Damak F., Camonis J., Garreau H., Jacquet M.
    Gene 77:21-30(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 670-1253.
    Strain: 0L136.
  3. "Enhancement of the GDP-GTP exchange of RAS proteins by the carboxyl-terminal domain of SCD25."
    Crechet J.B., Poullet P., Mistou M.-Y., Parmeggiani A., Camonis J., Boy-Marcotte E., Damak F., Jacquet M.
    Science 248:866-868(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "The COOH-domain of the product of the Saccharomyces cerevisiae SCD25 gene elicits activation of p21-ras proteins in mammalian cells."
    Rey I., Schweighoffer F., Barlat I., Camonis J., Boy-Marcotte E., Guilbaud R., Jacquet M., Tocque B.
    Oncogene 6:347-349(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "SDC25, a dispensable Ras guanine nucleotide exchange factor of Saccharomyces cerevisiae differs from CDC25 by its regulation."
    Boy-Marcotte E., Ikonomi P., Jacquet M.
    Mol. Biol. Cell 7:529-539(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSDC25_YEASX
AccessioniPrimary (citable) accession number: P0CF32
Secondary accession number(s): P14771
, Q12037, Q12065, Q6B0T5, Q6WV04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: June 24, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Suppresses the CDC25-5 mutation in yeast (restores cAMP level) and has similar functions as CDC25.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.