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P0CF32 (SDC25_YEASX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Guanine nucleotide exchange factor SDC25
Gene names
Name:SDC25
Synonyms:SCD25
OrganismSaccharomyces cerevisiae (Baker's yeast)
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1253 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes the exchange of Ras-bound GDP by GTP. Ref.3 Ref.4 Ref.5

Miscellaneous

Suppresses the CDC25-5 mutation in yeast (restores cAMP level) and has similar functions as CDC25.

Sequence similarities

Contains 1 N-terminal Ras-GEF domain.

Contains 1 Ras-GEF domain.

Contains 1 SH3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12531253Guanine nucleotide exchange factor SDC25
PRO_0000068893

Regions

Domain26 – 9772SH3
Domain782 – 914133N-terminal Ras-GEF
Domain952 – 1199248Ras-GEF

Natural variations

Natural variant682 – 69211KSCDDYFDVLK → NPVMTILMCLN in strain: 0L136.
Natural variant7401Missing in strain: 0L136.
Natural variant783 – 7853PIV → SNN in strain: 0L136.
Natural variant9741I → T in strain: 0L136.
Natural variant10481A → D in strain: 0L136.

Sequences

Sequence LengthMass (Da)Tools
P0CF32 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: 2DE2C9EC27E3E60D

FASTA1,253144,980
        10         20         30         40         50         60 
MSCTASYAGM TTPVKDKEGH GIPCLQPIDV VECTYQYFTK SRNKLSLRVG DLIYVLTKGS 

        70         80         90        100        110        120 
NGWWDGVLIR HSANNNNNNS LILDRGWFPP SFTRSILNEL HGVPDIGNEL EIFQAGLNLK 

       130        140        150        160        170        180 
LELSSNPVIL SLEDFLDCCR DIEFKEQLAW SPTPVHERKG CCELLYYNQD LDVYCRTLPY 

       190        200        210        220        230        240 
LPQNQVETVN DYSSFPAISK IAGKKMPITS SPDLFYLNDC DVVYWYDLTR LVCHYVNLTE 

       250        260        270        280        290        300 
RDLLANEREK FLTSLDLLTA QITYVYMLFR NLRLVEDSFK KTLKKLIYTL SRFSINANIW 

       310        320        330        340        350        360 
FHSTSFEERE AIASQKDPER RSPLLQSILG TFQKFHFLLR LLHFLSNPNE LTILPQLTPR 

       370        380        390        400        410        420 
FFKDSFNTIS WNNPFLRTVF NQHMSMTLPR QMIKAVAGAS GIVAENIDEI PASKQGTFIS 

       430        440        450        460        470        480 
SETSHHSPSA PFQRRRRGTI FSNVSGSSDE SDTIWSKRKK PYPLNEETLS LVRARKKQLD 

       490        500        510        520        530        540 
GKLKQMIKSA NEYLSNTANF SKMLNFEMNF KTYEEVSGTI PIIDILENLD LTIFLNLREL 

       550        560        570        580        590        600 
GDENRVFDED VAIGDEDKEF LKHSLSSLSY ILSDYFNMKQ YFHDVVVKFI IVAQHLTLED 

       610        620        630        640        650        660 
PFVFSPMQND LPTGYYEPMK PSSLNLDNAK DKKNGSQNTD IQEEEDEYEP DPDSLILFHN 

       670        680        690        700        710        720 
LINQDSDFND LKFFNLAHVF KKSCDDYFDV LKLAIEFVNQ LILERENLLN YAARMMKNNI 

       730        740        750        760        770        780 
TELLLRGEEG YGSYDGGETA EKSDTNAVYA DSDTKDNDEW RDSQVKLPRY LQREYDSELI 

       790        800        810        820        830        840 
WGPIVRIKGG SKHALISYLT DNEKKDLFFN ITFLITFRSI FTTTEFLSYL ISQYNLDPPE 

       850        860        870        880        890        900 
DLCFEEYNEW VTKKLIPVKC RVVEIMTTFF KQYWFPGYDE PDLATLNLDY FAQVAIKENI 

       910        920        930        940        950        960 
TGSVELLKEV NQKFKLGNIQ EATAPMKTLD QQICQDHYSG TLYSTTESIL AVDPVLFATQ 

       970        980        990       1000       1010       1020 
LTILEHEIYC EITIFDCLQK IWKNKYTKSY GASPGLNEFI SFANKLTNFI SYSVVKEADK 

      1030       1040       1050       1060       1070       1080 
SKRAKLLSHF IFIAEYCRKF NNFSSMTAII SALYSSPIYR LEKTWQAVIP QTRDLLQSLN 

      1090       1100       1110       1120       1130       1140 
KLMDPKKNFI NYRNELKSLH SAPCVPFFGV YLSDLTFTDS GNPDYLVLEH GLKGVHDEKK 

      1150       1160       1170       1180       1190       1200 
YINFNKRSRL VDILQEIIYF KKTHYDFTKD RTVIECISNS LENIPHIEKQ YQLSLIIEPK 

      1210       1220       1230       1240       1250 
PRKKVVPNSN SNNKSQEKSR DDQTDEGKTS TKKDRFPKFQ LHKTKKKAPK VSK 

« Hide

References

[1]"SDC25, a CDC25-like gene which contains a RAS-activating domain and is a dispensable gene of Saccharomyces cerevisiae."
Damak F., Boy-Marcotte E., le Roscouet D., Guilbaud R., Jacquet M.
Mol. Cell. Biol. 11:202-212(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 200060 / W303.
[2]"The C-terminal part of a gene partially homologous to CDC 25 gene suppresses the cdc25-5 mutation in Saccharomyces cerevisiae."
Boy-Marcotte E., Damak F., Camonis J., Garreau H., Jacquet M.
Gene 77:21-30(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 670-1253.
Strain: 0L136.
[3]"Enhancement of the GDP-GTP exchange of RAS proteins by the carboxyl-terminal domain of SCD25."
Crechet J.B., Poullet P., Mistou M.-Y., Parmeggiani A., Camonis J., Boy-Marcotte E., Damak F., Jacquet M.
Science 248:866-868(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"The COOH-domain of the product of the Saccharomyces cerevisiae SCD25 gene elicits activation of p21-ras proteins in mammalian cells."
Rey I., Schweighoffer F., Barlat I., Camonis J., Boy-Marcotte E., Guilbaud R., Jacquet M., Tocque B.
Oncogene 6:347-349(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"SDC25, a dispensable Ras guanine nucleotide exchange factor of Saccharomyces cerevisiae differs from CDC25 by its regulation."
Boy-Marcotte E., Ikonomi P., Jacquet M.
Mol. Biol. Cell 7:529-539(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M26647 Genomic DNA. Translation: AAA16565.1.
PIRS14177.

3D structure databases

ProteinModelPortalP0CF32.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP0CF32. 4 interactions.

Proteomic databases

PeptideAtlasP14771.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Gene expression databases

GenevestigatorP14771.

Family and domain databases

Gene3D1.10.840.10. 1 hit.
InterProIPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR008937. Ras_GEF.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR23113. PTHR23113. 1 hit.
PfamPF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTSM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48366. SSF48366. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEPS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSDC25_YEASX
AccessionPrimary (citable) accession number: P0CF32
Secondary accession number(s): P14771 expand/collapse secondary AC list , Q12037, Q12065, Q6B0T5, Q6WV04
Entry history
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: April 16, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families