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Protein

Insertion element IS1 3 protein InsB

Gene

insB3

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Absolutely required for transposition of IS1.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA recombination, Transposition

Names & Taxonomyi

Protein namesi
Recommended name:
Insertion element IS1 3 protein InsB
Alternative name(s):
IS1c
Gene namesi
Name:insB3
Ordered Locus Names:b0274, JW0268
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG40002. insB3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 167167Insertion element IS1 3 protein InsBPRO_0000393402Add
BLAST

Proteomic databases

PaxDbiP0CF27.
PRIDEiP0CF27.

Interactioni

Protein-protein interaction databases

STRINGi511145.b0274.

Family & Domainsi

Sequence similaritiesi

Belongs to the transposase 27 family.Curated

Phylogenomic databases

eggNOGiENOG4108MBK. Bacteria.
COG1662. LUCA.
HOGENOMiHOG000036821.
InParanoidiP0CF27.
KOiK07480.
PhylomeDBiP0CF27.

Family and domain databases

InterProiIPR005063. Transposase_27.
[Graphical view]
PfamiPF03400. DDE_Tnp_IS1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CF27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGNRPHYGR WPQHDFPPFK KLRPQSVTSR IQPGSDVIVC AEMDEQWGYV
60 70 80 90 100
GAKSRQRWLF YAYDRLRKTV VAHVFGERTM ATLGRLMSLL SPFDVVIWMT
110 120 130 140 150
DGWPLYESRL KGKLHVISKR YTQRIERHNL NLRQHLARLG RKSLSFSKSV
160
EQHDKVIGHY LNIKHYQ
Length:167
Mass (Da):19,758
Last modified:April 20, 2010 - v1
Checksum:iB1CA4E4035F49368
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52535 Genomic DNA. No translation available.
U70214 Genomic DNA. Translation: AAB08684.1.
U00096 Genomic DNA. Translation: AAC73377.1.
AP009048 Genomic DNA. Translation: BAE76058.1.
PIRiJN0137. IEECA1.
RefSeqiNP_414798.1. NC_000913.3.
NP_414808.1. NC_000913.3.
WP_001119292.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73377; AAC73377; b0274.
BAE76058; BAE76058; BAE76058.
GeneIDi944950.
947698.
KEGGiecj:JW0268.
eco:b0264.
eco:b0274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52535 Genomic DNA. No translation available.
U70214 Genomic DNA. Translation: AAB08684.1.
U00096 Genomic DNA. Translation: AAC73377.1.
AP009048 Genomic DNA. Translation: BAE76058.1.
PIRiJN0137. IEECA1.
RefSeqiNP_414798.1. NC_000913.3.
NP_414808.1. NC_000913.3.
WP_001119292.1. NZ_LN832404.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b0274.

Proteomic databases

PaxDbiP0CF27.
PRIDEiP0CF27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73377; AAC73377; b0274.
BAE76058; BAE76058; BAE76058.
GeneIDi944950.
947698.
KEGGiecj:JW0268.
eco:b0264.
eco:b0274.

Organism-specific databases

EchoBASEiEB4707.
EcoGeneiEG40002. insB3.

Phylogenomic databases

eggNOGiENOG4108MBK. Bacteria.
COG1662. LUCA.
HOGENOMiHOG000036821.
InParanoidiP0CF27.
KOiK07480.
PhylomeDBiP0CF27.

Miscellaneous databases

PROiP0CF27.

Family and domain databases

InterProiIPR005063. Transposase_27.
[Graphical view]
PfamiPF03400. DDE_Tnp_IS1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Four types of IS1 with differences in nucleotide sequence reside in the Escherichia coli K-12 chromosome."
    Umeda M., Ohtsubo E.
    Gene 98:1-5(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (B0264 AND B0274).
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiINSB3_ECOLI
AccessioniPrimary (citable) accession number: P0CF27
Secondary accession number(s): Q2MCE8, Q47302, Q47691
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: November 11, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome, Transposable element

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.