P0CF26 (INSB2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insertion element IS1 2 protein InsB

Alternative name(s):
IS1b

Gene names

Name:	insB2
Ordered Locus Names:	b0264, JW0256

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Absolutely required for transposition of IS1.
Sequence similarities	Belongs to the transposase 27 family.

Ontologies

Keywords
Biological process	DNA recombination Transposition
Technical term	Complete proteome Reference proteome Transposable element
Gene Ontology (GO)
Biological_process	transposition, DNA-mediated Inferred from mutant phenotype PubMed 8083181. Source: EcoCyc
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro transposase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 167	167	Insertion element IS1 2 protein InsB		PRO_0000075402

Sequences

	Sequence		Length	Mass (Da)
	P0CF26 [UniParc]. Last modified April 20, 2010. Version 1. Checksum: B1CA4E4035F49368	FASTA	167	19,758
	10 20 30 40 50 60 MPGNRPHYGR WPQHDFPPFK KLRPQSVTSR IQPGSDVIVC AEMDEQWGYV GAKSRQRWLF 70 80 90 100 110 120 YAYDRLRKTV VAHVFGERTM ATLGRLMSLL SPFDVVIWMT DGWPLYESRL KGKLHVISKR 130 140 150 160 YTQRIERHNL NLRQHLARLG RKSLSFSKSV EQHDKVIGHY LNIKHYQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Four types of IS1 with differences in nucleotide sequence reside in the Escherichia coli K-12 chromosome." Umeda M., Ohtsubo E. Gene 98:1-5(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The delta (argF-lacZ)205(U169) deletion greatly enhances resistance to hydrogen peroxide in stationary-phase Escherichia coli." Volkert M.R., Loewen P.C., Switala J., Crowley D., Conley M. J. Bacteriol. 176:1297-1302(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L20943 Unassigned DNA. Translation: AAA69643.1. U70214 Genomic DNA. Translation: AAB08695.1. U00096 Genomic DNA. Translation: AAC73367.1. AP009048 Genomic DNA. Translation: BAE76050.1.
RefSeq	NP_414798.1. NC_000913.2. NP_414808.1. NC_000913.2. YP_488559.1. NC_007779.1. YP_488570.1. NC_007779.1.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P0CF26.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73367; AAC73367; b0264. BAE76050; BAE76050; BAE76050.
GeneID	12932567. 12933818. 944950. 947698.
KEGG	ecj:Y75_p0255. ecj:Y75_p0266. eco:b0264. eco:b0274.
Organism-specific databases
EchoBASE	EB4705.
EcoGene	EG40002. insB2.
Phylogenomic databases
HOGENOM	HOG000036821.
KO	K07480.
Enzyme and pathway databases
BioCyc	EcoCyc:G6138-MONOMER.
Family and domain databases
InterPro	IPR005063. Transposase_27. [Graphical view]
Pfam	PF03400. DDE_Tnp_IS1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INSB2_ECOLI

Accession

Primary (citable) accession number: P0CF26
Secondary accession number(s): Q2MCE8, Q47302, Q47691

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	April 20, 2010
Last modified:	May 1, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program