P0CF08 (INSA2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insertion element IS1 2 protein InsA

Alternative name(s):
IS1b

Gene names

Name:	insA2
Ordered Locus Names:	b0265, JW0257

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	91 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Absolutely required for transposition of IS1.
Sequence similarities	Belongs to the IS1 elements InsA family.

Ontologies

Keywords
Biological process	DNA recombination Transposition
Technical term	Complete proteome Reference proteome Transposable element
Gene Ontology (GO)
Biological_process	transposition, DNA-mediated Inferred from mutant phenotype PubMed 8083181. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 91	91	Insertion element IS1 2 protein InsA		PRO_0000316158

Sequences

	Sequence		Length	Mass (Da)
	P0CF08 [UniParc]. Last modified April 20, 2010. Version 1. Checksum: 8249502B66B91FC5	FASTA	91	9,902
	10 20 30 40 50 60 MASVSISCPS CSATDGVVRN GKSTAGHQRY LCSHCRKTWQ LQFTYTASQP GTHQKIIDMA 70 80 90 MNGVGCRATA RIMGVGLNTI FRHLKNSGRS R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mapping of insertion element IS30 in the Escherichia coli K12 chromosome." Umeda M., Ohtsubo E. Mol. Gen. Genet. 222:317-322(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The delta (argF-lacZ)205(U169) deletion greatly enhances resistance to hydrogen peroxide in stationary-phase Escherichia coli." Volkert M.R., Loewen P.C., Switala J., Crowley D., Conley M. J. Bacteriol. 176:1297-1302(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L20943 Unassigned DNA. Translation: AAA69642.1. X17345 Genomic DNA. Translation: CAA35227.1. U70214 Genomic DNA. Translation: AAB08685.1. U00096 Genomic DNA. Translation: AAC73368.1. AP009048 Genomic DNA. Translation: BAE76051.1.
PIR	IEECB9. B93826. JN0134.
RefSeq	NP_414799.1. NC_000913.2. NP_414809.1. NC_000913.2. NP_862965.1. NC_004998.1. NP_863031.1. NC_004998.1. NP_957637.1. NC_005327.1. YP_001096420.1. NC_009133.1. YP_001096518.1. NC_009133.1. YP_002527566.1. NC_011964.1. YP_003108318.1. NC_013122.1. YP_006953891.1. NC_019090.1. YP_006954228.1. NC_019095.1. YP_006990704.1. NC_019424.1. YP_007447506.1. NC_020278.1. YP_488560.1. NC_007779.1. YP_488571.1. NC_007779.1. YP_489259.1. NC_007779.1. YP_588445.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0CF08.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73368; AAC73368; b0265. BAE76051; BAE76051; BAE76051.
GeneID	12930785. 12932558. 12933049. 13906835. 13906917. 13982288. 1446608. 1446614. 1450250. 14612516. 2716512. 4924745. 4924843. 7324590. 8319248. 944946. 944951.
KEGG	ecj:Y75_p0256. ecj:Y75_p0267. ecj:Y75_p0959. eco:b0265. eco:b0275. eco:b4516.
Organism-specific databases
EchoBASE	EB4706.
EcoGene	EG40001. insA2.
Phylogenomic databases
OMA	YLCSHCH.
Enzyme and pathway databases
BioCyc	EcoCyc:G6139-MONOMER.
Family and domain databases
InterPro	IPR024431. InsA_HTH_dom. IPR003220. IS1_InsA_N. [Graphical view]
Pfam	PF12759. HTH_Tnp_IS1. 1 hit. PF03811. Zn_Tnp_IS1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

INSA2_ECOLI

Accession

Primary (citable) accession number: P0CF08
Secondary accession number(s): P03827 , P0ADH0, P0C651, Q2EER2, Q2MCF5, Q2MCH2, Q933I5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	April 20, 2010
Last modified:	May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program