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Protein

Zinc transporter 8

Gene

Slc30a8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter (By similarity). May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta-cells.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • cellular zinc ion homeostasis Source: BHF-UCL
  • insulin secretion Source: RGD
  • positive regulation of insulin secretion Source: BHF-UCL
  • regulation of sequestering of zinc ion Source: BHF-UCL
  • regulation of vesicle-mediated transport Source: BHF-UCL
  • response to glucose Source: BHF-UCL
  • response to interferon-gamma Source: RGD
  • response to interleukin-1 Source: RGD
  • response to zinc ion Source: GO_Central
  • sequestering of zinc ion Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport, Zinc transport

Keywords - Ligandi

Zinc

Enzyme and pathway databases

ReactomeiR-RNO-264876. Insulin processing.
R-RNO-435368. Zinc efflux and compartmentalization by the SLC30 family.

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc transporter 8
Short name:
ZnT-8
Alternative name(s):
Solute carrier family 30 member 8
Gene namesi
Name:Slc30a8
Synonyms:Znt8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1308282. Slc30a8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7878CytoplasmicSequence analysisAdd
BLAST
Transmembranei79 – 9921HelicalSequence analysisAdd
BLAST
Topological domaini100 – 1023ExtracellularSequence analysis
Transmembranei103 – 12321HelicalSequence analysisAdd
BLAST
Topological domaini124 – 13916CytoplasmicSequence analysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence analysisAdd
BLAST
Topological domaini161 – 17414ExtracellularSequence analysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence analysisAdd
BLAST
Topological domaini196 – 21621CytoplasmicSequence analysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence analysisAdd
BLAST
Topological domaini238 – 2458ExtracellularSequence analysis
Transmembranei246 – 26621HelicalSequence analysisAdd
BLAST
Topological domaini267 – 368102CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Zinc transporter 8PRO_0000392208Add
BLAST

Proteomic databases

PaxDbiP0CE46.

Expressioni

Tissue specificityi

Expressed in endocrine pancreatic islet alpha and beta cells. May be more abundant in beta cells than in alpha cells. Expressed in cubical epithelium lining thyroid follicles (at protein level). In the adrenal gland, detected in the cortex, but not in the medulla (at protein level).1 Publication

Inductioni

Down-regulated by IL1B and IFNG.1 Publication

Gene expression databases

ExpressionAtlasiP0CE46. baseline and differential.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006410.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi196 – 2049HXXXXX[HY]NH

Domaini

Contains a histidine-rich region, HXXXXXHNH-motif, which is a ligand for zinc.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1482. Eukaryota.
COG1230. LUCA.
GeneTreeiENSGT00550000074381.
InParanoidiP0CE46.
KOiK14695.
OMAiHNHGSHN.
OrthoDBiEOG7S4X68.
PhylomeDBiP0CE46.
TreeFamiTF313382.

Family and domain databases

Gene3Di1.20.1510.10. 1 hit.
InterProiIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
IPR033572. ZnT-8.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 1 hit.
PTHR11562:SF37. PTHR11562:SF37. 1 hit.
PfamiPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.

Sequencei

Sequence statusi: Complete.

P0CE46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFLERTYLV NDQATKMYAF TSDRERGQKP VNKDQCPGDG PERPEAGAIY
60 70 80 90 100
HCHNSFKATG NRSSKQVHAK WRLCAASAIC FFFMVAEVVG GHVAGSLAVL
110 120 130 140 150
TDAAHLLIDL TSFLLSLFSL WLSSRPPSKR LTFGWYRAEI LGALLSVLCI
160 170 180 190 200
WVVTGVLVYL ACERLLYPDY QIQAGIMITV SGCAVAANIV LTLILHQRHL
210 220 230 240 250
GHNHKDAQAN ASVRAAFVHA LGDVFQSTSV LISALIIYFK PDYKMADPVC
260 270 280 290 300
TFISSVLALA STVMILKDFS ILLMEGVPKG LSYNSVKELL LTVDGVISVH
310 320 330 340 350
NLHIWSLTVN QVILSVHVAT AASQDSQSVR TGIACALSSS FDLHSLTIQI
360
ESAADQDPSC LLCEDPQD
Length:368
Mass (Da):40,128
Last modified:March 23, 2010 - v1
Checksum:iC1E62FDCB524AFAE
GO

Sequence cautioni

The sequence EDM16273.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473950 Genomic DNA. Translation: EDM16273.1. Sequence problems.
RefSeqiNP_001124010.1. NM_001130538.1.
XP_006241707.1. XM_006241645.2.
UniGeneiRn.220601.

Genome annotation databases

EnsembliENSRNOT00000079792; ENSRNOP00000069455; ENSRNOG00000004747.
GeneIDi299903.
KEGGirno:299903.
UCSCiRGD:1308282. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473950 Genomic DNA. Translation: EDM16273.1. Sequence problems.
RefSeqiNP_001124010.1. NM_001130538.1.
XP_006241707.1. XM_006241645.2.
UniGeneiRn.220601.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006410.

Proteomic databases

PaxDbiP0CE46.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000079792; ENSRNOP00000069455; ENSRNOG00000004747.
GeneIDi299903.
KEGGirno:299903.
UCSCiRGD:1308282. rat.

Organism-specific databases

CTDi169026.
RGDi1308282. Slc30a8.

Phylogenomic databases

eggNOGiKOG1482. Eukaryota.
COG1230. LUCA.
GeneTreeiENSGT00550000074381.
InParanoidiP0CE46.
KOiK14695.
OMAiHNHGSHN.
OrthoDBiEOG7S4X68.
PhylomeDBiP0CE46.
TreeFamiTF313382.

Enzyme and pathway databases

ReactomeiR-RNO-264876. Insulin processing.
R-RNO-435368. Zinc efflux and compartmentalization by the SLC30 family.

Miscellaneous databases

PROiP0CE46.

Gene expression databases

ExpressionAtlasiP0CE46. baseline and differential.

Family and domain databases

Gene3Di1.20.1510.10. 1 hit.
InterProiIPR002524. Cation_efflux.
IPR027469. Cation_efflux_TMD.
IPR033572. ZnT-8.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 1 hit.
PTHR11562:SF37. PTHR11562:SF37. 1 hit.
PfamiPF01545. Cation_efflux. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Diabetes-linked zinc transporter ZnT8 is a homodimeric protein expressed by distinct rodent endocrine cell types in the pancreas and other glands."
    Murgia C., Devirgiliis C., Mancini E., Donadel G., Zalewski P., Perozzi G.
    Nutr. Metab. Cardiovasc. Dis. 19:431-439(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "Down-regulation of ZnT8 expression in INS-1 rat pancreatic beta cells reduces insulin content and glucose-inducible insulin secretion."
    Fu Y., Tian W., Pratt E.B., Dirling L.B., Shyng S.L., Meshul C.K., Cohen D.M.
    PLoS ONE 4:E5679-E5679(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Zinc transporter gene expression is regulated by pro-inflammatory cytokines: a potential role for zinc transporters in beta-cell apoptosis?"
    Egefjord L., Jensen J.L., Bang-Berthelsen C.H., Petersen A.B., Smidt K., Schmitz O., Karlsen A.E., Pociot F., Chimienti F., Rungby J., Magnusson N.E.
    BMC Endocr. Disord. 9:7-7(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY IL1B AND IFNG.

Entry informationi

Entry nameiZNT8_RAT
AccessioniPrimary (citable) accession number: P0CE46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: March 23, 2010
Last modified: July 6, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.