Alpha-glucosides permease MPH3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P0CE00 (MPH3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 25. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-glucosides permease MPH3

Alternative name(s):
Maltose transport protein 3

Gene names

Name:	MPH3
Ordered Locus Names:	YJR160C
ORF Names:	J2400

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High-affinity uptake of maltose and maltotriose. Also transports alpha-methylglucoside, glucose and turanose but not melezitose or trehalose. Ref.3 Ref.4
Subcellular location	Cell membrane By similarity; Multi-pass membrane protein.
Induction	By maltose and maltotriose. Repressed by glucose. Ref.4
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Ontologies

Keywords
Biological process	Maltose metabolism Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maltose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD plasma membrane Inferred from sequence or structural similarity Ref.4. Source: SGD
Molecular_function	alpha-glucoside transmembrane transporter activity Inferred from direct assay Ref.4. Source: UniProtKB maltose transmembrane transporter activity Inferred from sequence or structural similarity Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 602

602

Alpha-glucosides permease MPH3

PRO_0000391710

Regions

Topological domain

1 – 106

106

Cytoplasmic Potential

Transmembrane

107 – 127

Helical; Name=1; Potential

Topological domain

128 – 142

Extracellular Potential

Transmembrane

143 – 163

Helical; Name=2; Potential

Topological domain

164 – 178

Cytoplasmic Potential

Transmembrane

179 – 199

Helical; Name=3; Potential

Topological domain

200

Extracellular Potential

Transmembrane

201 – 221

Helical; Name=4; Potential

Topological domain

222 – 234

Cytoplasmic Potential

Transmembrane

235 – 255

Helical; Name=5; Potential

Topological domain

256 – 270

Extracellular Potential

Transmembrane

271 – 291

Helical; Name=6; Potential

Topological domain

292 – 363

Cytoplasmic Potential

Transmembrane

364 – 384

Helical; Name=7; Potential

Topological domain

385 – 397

Extracellular Potential

Transmembrane

398 – 418

Helical; Name=8; Potential

Topological domain

419 – 426

Cytoplasmic Potential

Transmembrane

427 – 447

Helical; Name=9; Potential

Topological domain

448 – 459

Extracellular Potential

Transmembrane

460 – 480

Helical; Name=10; Potential

Topological domain

481 – 492

Cytoplasmic Potential

Transmembrane

493 – 513

Helical; Name=11; Potential

Topological domain

514 – 525

Extracellular Potential

Transmembrane

526 – 546

Helical; Name=12; Potential

Topological domain

547 – 602

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P0CE00 [UniParc].

Last modified March 2, 2010. Version 1.
Checksum: 75BDC04C164CFDF3
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FASTA

602

67,257

        10         20         30         40         50         60 
MKNLSFLINR RKENTSDSNV YPGKAKSHEP SWIEMDDQTK KDGLDIVHVE FSPDTRAPSD 

        70         80         90        100        110        120 
SNKVITEIFD ATEDAKEADE SERGMPLATA LNTYPKAAAW SLLVSTTLIM EGYDTAILGA 

       130        140        150        160        170        180 
FYALPIFQRK FGSQNDKTGE WEISASWQIG LTLCYMAGEI VGLQLTGPSV DLVGNRYTLI 

       190        200        210        220        230        240 
IALFFLAAFT FILYFCNSLG MIAVGQALCG MPWGCFQCLT VSYASEICPL ALRYYLTTYS 

       250        260        270        280        290        300 
NLCWLFGQLF AAGIMKNSQK KYADSELGYK LPFALQWILP VPLALGIFFA PESPWWLVKK 

       310        320        330        340        350        360 
GRFDEARRSL RRTLSGKGPE KEILVTLEVD KIKVTIDKEK RLTSKEGSYS DCFEDKINRR 

       370        380        390        400        410        420 
RTRITCLCWA GQATCGSILI GYSTYFYEKA GVSTEMSFTF SIIQYCLGIC ATFLSWWASK 

       430        440        450        460        470        480 
YFGRYDLYAF GLAFQTIVFF IIGGLGCSST HGSKMGSGSL LMAVAFFYNL GIAPVVFCLV 

       490        500        510        520        530        540 
SEMPSSRLRT KTIILARNTY NVVSIICSVL ILYQLNSKKW NWGAKSGFFW GVLCFCTLIW 

       550        560        570        580        590        600 
AVVDLPETAG KTFVEINELF KLGVSARKFK STKVDPFVVK TPPKDVSHND PKGDIEASIA 


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« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae." Wieczorke R., Krampe S., Weierstall T., Freidel K., Hollenberg C.P., Boles E. FEBS Lett. 464:123-128(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Characterization of the putative maltose transporters encoded by YDL247w and YJR160c." Day R.E., Higgins V.J., Rogers P.J., Dawes I.W. Yeast 19:1015-1027(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[5]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49660 Genomic DNA. Translation: CAA89693.1. BK006943 Genomic DNA. Translation: DAA08944.1.
PIR	S57190.
RefSeq	NP_012694.1. NM_001181818.1.
3D structure databases
ProteinModelPortal	P0CE00.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR160C; YJR160C; YJR160C.
GeneID	853625.
KEGG	sce:YJR160C.
Organism-specific databases
SGD	S000003921. MPH3.
Phylogenomic databases
GeneTree	ENSGT00650000093912.
HOGENOM	HOG000159003.
KO	K08141.
OMA	GEEVETH.
OrthoDB	EOG454D72.
Gene expression databases
Genevestigator	P47186.
GermOnline	YJR160C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR005828. Sub_transporter. IPR003663. Sugar/inositol_transpt. IPR005829. Sugar_transporter_CS. [Graphical view]
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00879. SP. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. False negative. PS00217. SUGAR_TRANSPORT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974493.

Entry information

Entry name

MPH3_YEAST

Accession

Primary (citable) accession number: P0CE00
Secondary accession number(s): D6VWX8, P47186, Q07787

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 2, 2010
Last sequence update:	March 2, 2010
Last modified:	April 3, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents