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P0CE00 (MPH3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-glucosides permease MPH3
Alternative name(s):
Maltose transport protein 3
Gene names
Name:MPH3
Ordered Locus Names:YJR160C
ORF Names:J2400
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High-affinity uptake of maltose and maltotriose. Also transports alpha-methylglucoside, glucose and turanose but not melezitose or trehalose. Ref.3 Ref.4

Subcellular location

Cell membrane By similarity; Multi-pass membrane protein.

Induction

By maltose and maltotriose. Repressed by glucose. Ref.4

Sequence similarities

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 602602Alpha-glucosides permease MPH3
PRO_0000391710

Regions

Topological domain1 – 106106Cytoplasmic Potential
Transmembrane107 – 12721Helical; Name=1; Potential
Topological domain128 – 14215Extracellular Potential
Transmembrane143 – 16321Helical; Name=2; Potential
Topological domain164 – 17815Cytoplasmic Potential
Transmembrane179 – 19921Helical; Name=3; Potential
Topological domain2001Extracellular Potential
Transmembrane201 – 22121Helical; Name=4; Potential
Topological domain222 – 23413Cytoplasmic Potential
Transmembrane235 – 25521Helical; Name=5; Potential
Topological domain256 – 27015Extracellular Potential
Transmembrane271 – 29121Helical; Name=6; Potential
Topological domain292 – 36372Cytoplasmic Potential
Transmembrane364 – 38421Helical; Name=7; Potential
Topological domain385 – 39713Extracellular Potential
Transmembrane398 – 41821Helical; Name=8; Potential
Topological domain419 – 4268Cytoplasmic Potential
Transmembrane427 – 44721Helical; Name=9; Potential
Topological domain448 – 45912Extracellular Potential
Transmembrane460 – 48021Helical; Name=10; Potential
Topological domain481 – 49212Cytoplasmic Potential
Transmembrane493 – 51321Helical; Name=11; Potential
Topological domain514 – 52512Extracellular Potential
Transmembrane526 – 54621Helical; Name=12; Potential
Topological domain547 – 60256Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P0CE00 [UniParc].

Last modified March 2, 2010. Version 1.
Checksum: 75BDC04C164CFDF3

FASTA60267,257
        10         20         30         40         50         60 
MKNLSFLINR RKENTSDSNV YPGKAKSHEP SWIEMDDQTK KDGLDIVHVE FSPDTRAPSD 

        70         80         90        100        110        120 
SNKVITEIFD ATEDAKEADE SERGMPLATA LNTYPKAAAW SLLVSTTLIM EGYDTAILGA 

       130        140        150        160        170        180 
FYALPIFQRK FGSQNDKTGE WEISASWQIG LTLCYMAGEI VGLQLTGPSV DLVGNRYTLI 

       190        200        210        220        230        240 
IALFFLAAFT FILYFCNSLG MIAVGQALCG MPWGCFQCLT VSYASEICPL ALRYYLTTYS 

       250        260        270        280        290        300 
NLCWLFGQLF AAGIMKNSQK KYADSELGYK LPFALQWILP VPLALGIFFA PESPWWLVKK 

       310        320        330        340        350        360 
GRFDEARRSL RRTLSGKGPE KEILVTLEVD KIKVTIDKEK RLTSKEGSYS DCFEDKINRR 

       370        380        390        400        410        420 
RTRITCLCWA GQATCGSILI GYSTYFYEKA GVSTEMSFTF SIIQYCLGIC ATFLSWWASK 

       430        440        450        460        470        480 
YFGRYDLYAF GLAFQTIVFF IIGGLGCSST HGSKMGSGSL LMAVAFFYNL GIAPVVFCLV 

       490        500        510        520        530        540 
SEMPSSRLRT KTIILARNTY NVVSIICSVL ILYQLNSKKW NWGAKSGFFW GVLCFCTLIW 

       550        560        570        580        590        600 
AVVDLPETAG KTFVEINELF KLGVSARKFK STKVDPFVVK TPPKDVSHND PKGDIEASIA 


EE 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae."
Wieczorke R., Krampe S., Weierstall T., Freidel K., Hollenberg C.P., Boles E.
FEBS Lett. 464:123-128(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Characterization of the putative maltose transporters encoded by YDL247w and YJR160c."
Day R.E., Higgins V.J., Rogers P.J., Dawes I.W.
Yeast 19:1015-1027(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49660 Genomic DNA. Translation: CAA89693.1.
BK006943 Genomic DNA. Translation: DAA08944.1.
PIRS57190.
RefSeqNP_012694.1. NM_001181818.1.

3D structure databases

ProteinModelPortalP0CE00.
SMRP0CE00. Positions 156-550.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33914. 42 interactions.
MINTMINT-565339.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR160C; YJR160C; YJR160C.
GeneID853625.
KEGGsce:YJR160C.

Organism-specific databases

SGDS000003921. MPH3.

Phylogenomic databases

GeneTreeENSGT00720000109429.
HOGENOMHOG000159003.
KOK08141.
OMAGMDASNS.
OrthoDBEOG7MKWG3.

Enzyme and pathway databases

BioCycYEAST:G3O-31769-MONOMER.

Gene expression databases

GenevestigatorP47186.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 2 hits.
TIGRFAMsTIGR00879. SP. 1 hit.
PROSITEPS50850. MFS. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974493.

Entry information

Entry nameMPH3_YEAST
AccessionPrimary (citable) accession number: P0CE00
Secondary accession number(s): D6VWX8, P47186, Q07787
Entry history
Integrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: March 2, 2010
Last modified: May 14, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families