P0CD90 (AQY2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aquaporin-like protein 2

Alternative name(s):
Truncated aquaporin-2

Gene names

Name:	AQY2
Synonyms:	AQY2-2
Ordered Locus Names:	YLL052C
ORF Names:	L0590

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Water channel required to facilitate the transport of water across membranes. Involved in freeze tolerance, osmotolerance and cell flocculation in liquid cultures By similarity. Is non-functional in most laboratory strains.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane By similarity; Multi-pass membrane protein Ref.5.
Induction	During exponential phase in rich medium and repressed in minimum medium, hyper-osmolar medium or in sporulating conditions By similarity.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]
Caution	This is a truncated version of aquaporin-2. In strain S288c and many laboratory strains, a natural 11 bp deletion in position 109 leads to a frameshift, which disrupts the gene coding for this protein and produces two ORFs YLL052C and YLL053C. A contiguous sequence for aquaporin-2 can be found in strain Sigma 1278B (AC P0CD89).

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Endoplasmic reticulum Membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from direct assay PubMed 11168368. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 11168368. Source: SGD
Molecular_function	water channel activity Inferred from sequence or structural similarity Ref.3. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	Aquaporin-like protein 2		PRO_0000391651
Regions
	Topological domain	1 – 47	47	Cytoplasmic By similarity
	Transmembrane	48 – 68	21	Helical; Potential
	Topological domain	69 – 89	21	Extracellular By similarity
	Transmembrane	90 – 110	21	Helical; Potential
	Topological domain	111 – 149	39	Cytoplasmic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0CD90 [UniParc]. Last modified March 2, 2010. Version 1. Checksum: 197E5A42A2F7495E	FASTA	149	17,024
	10 20 30 40 50 60 MSNESNDLEK NISHLDPTGV DNAYIPPEQP ETKHSRFNID RDTLRNHFIA AVGEFCGTFM 70 80 90 100 110 120 FLWCAYVICN VANHDVALTT EPEGSHPGQL IMIALGFGFS VMFSIWCFWW GFEPSRFSLF 130 140 VFGQSHLTSQ MCSDVVSSDH CWDGCWWCR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Polymorphism of Saccharomyces cerevisiae aquaporins." Laize V., Tacnet F., Ripoche P., Hohmann S. Yeast 16:897-903(2000) [PubMed] [Europe PMC] [Abstract] Cited for: POLYMORPHISM.
[4]	"Aquaporins in Saccharomyces: characterization of a second functional water channel protein." Carbrey J.M., Bonhivers M., Boeke J.D., Agre P. Proc. Natl. Acad. Sci. U.S.A. 98:1000-1005(2001) [PubMed] [Europe PMC] [Abstract] Cited for: POLYMORPHISM.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73158 Genomic DNA. Translation: CAA97506.1. Z47973 Genomic DNA. Translation: CAA88005.1. BK006945 Genomic DNA. Translation: DAA09272.1.
PIR	S50968.
RefSeq	NP_013048.1. NM_001181872.1.
3D structure databases
ProteinModelPortal	P0CD90.
SMR	P0CD90. Positions 16-106.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLL052C; YLL052C; YLL052C.
GeneID	850674.
KEGG	sce:YLL052C.
Organism-specific databases
SGD	S000003975. AQY2.
Phylogenomic databases
GeneTree	ENSGT00550000074347.
KO	K09885.
OrthoDB	EOG4TQQK7.
Gene expression databases
ArrayExpress	P0CD90.
Genevestigator	O93938.
Family and domain databases
Gene3D	1.20.1080.10. 1 hit.
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. [Graphical view]
PANTHER	PTHR19139. PTHR19139. 1 hit.
SUPFAM	SSF81338. MIP. 1 hit.
PROSITE	PS00221. MIP. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	966665.

Entry information

Entry name

AQY2_YEAST

Accession

Primary (citable) accession number: P0CD90
Secondary accession number(s): D6VXV6 , O93938, Q12258, Q12302, Q9C411

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 2, 2010
Last sequence update:	March 2, 2010
Last modified:	May 1, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program