Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UDP-3-O-acylglucosamine N-acyltransferase

Gene

lpxD

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the N-acylation of UDP-3-O-myristoylglucosamine using 3-hydroxyarachidoyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell (Probable).1 Publication

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein].

Pathwayi: LPS lipid A biosynthesis

This protein is involved in the pathway LPS lipid A biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS lipid A biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei43Participates in a stacking interaction with the uracil ring of UDP-GlcNAc1
Sitei49Participates in a stacking interaction with the uracil ring of UDP-GlcNAc1
Binding sitei240Palmitate1 Publication1
Binding sitei244Palmitate1 Publication1
Active sitei247Proton acceptor1 Publication1
Binding sitei248UDP-GlcNAc1 Publication1
Binding sitei266UDP-GlcNAc1 Publication1
Binding sitei284UDP-GlcNAc1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.191. 1315.
UniPathwayiUPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase (EC:2.3.1.-)
Gene namesi
Name:lpxD
Ordered Locus Names:CT_243
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000431 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000596651 – 354UDP-3-O-acylglucosamine N-acyltransferaseAdd BLAST354

Interactioni

Subunit structurei

Homotrimer.1 Publication

Protein-protein interaction databases

DIPiDIP-60889N.
STRINGi272561.CT243.

Structurei

Secondary structure

1354
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 14Combined sources7
Beta strandi18 – 21Combined sources4
Turni33 – 35Combined sources3
Beta strandi40 – 43Combined sources4
Beta strandi46 – 48Combined sources3
Helixi50 – 54Combined sources5
Beta strandi59 – 64Combined sources6
Helixi65 – 69Combined sources5
Beta strandi78 – 83Combined sources6
Helixi85 – 93Combined sources9
Turni94 – 96Combined sources3
Beta strandi117 – 119Combined sources3
Beta strandi169 – 173Combined sources5
Beta strandi184 – 188Combined sources5
Beta strandi192 – 195Combined sources4
Turni196 – 198Combined sources3
Beta strandi199 – 202Combined sources4
Beta strandi209 – 211Combined sources3
Beta strandi222 – 225Combined sources4
Beta strandi227 – 229Combined sources3
Beta strandi231 – 233Combined sources3
Beta strandi291 – 293Combined sources3
Beta strandi307 – 310Combined sources4
Turni311 – 314Combined sources4
Helixi317 – 328Combined sources12
Helixi330 – 342Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IU8X-ray2.20A/B/C1-354[»]
2IU9X-ray3.10A/B/C1-354[»]
2IUAX-ray2.70A/B/C1-354[»]
ProteinModelPortaliP0CD76.
SMRiP0CD76.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CD76.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 192UDP-GlcNAc binding3

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105D7M. Bacteria.
COG1044. LUCA.
InParanoidiP0CD76.
KOiK02536.
OMAiSYPAKIM.

Family and domain databases

CDDicd03352. LbH_LpxD. 1 hit.
HAMAPiMF_00523. LpxD. 1 hit.
InterProiIPR001451. Hexapep.
IPR007691. LpxD.
IPR011004. Trimer_LpxA-like.
IPR020573. UDP_GlcNAc_AcTrfase_non-rep.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF04613. LpxD. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01853. lipid_A_lpxD. 1 hit.

Sequencei

Sequence statusi: Complete.

P0CD76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSTYSLEQ LADFLKVEFQ GNGATLLSGV EEIEEAKTAH ITFLDNEKYA
60 70 80 90 100
KHLKSSEAGA IIISRTQFQK YRDLNKNFLI TSESPSLVFQ KCLELFITPV
110 120 130 140 150
DSGFPGIHPT AVIHPTAIIE DHVCIEPYAV VCQHAHVGSA CHIGSGSVIG
160 170 180 190 200
AYSTVGEHSY IHPRVVIRER VSIGKRVIIQ PGAVIGSCGF GYVTSAFGQH
210 220 230 240 250
KHLKHLGKVI IEDDVEIGAN TTIDRGRFKH SVVREGSKID NLVQIAHQVE
260 270 280 290 300
VGQHSMIVAQ AGIAGSTKIG NHVIIGGQAG ITGHICIADH VIMMAQTGVT
310 320 330 340 350
KSITSPGIYG GAPARPYQEI HRQVAKVRNL PRLEERIAAL EKLVQKLEAL

SEQH
Length:354
Mass (Da):38,404
Last modified:February 9, 2010 - v1
Checksum:iB9C547C129AE17BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67836.1.
PIRiD71539.
RefSeqiNP_219748.1. NC_000117.1.
WP_009871590.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC67836; AAC67836; CT_243.
GeneIDi884880.
KEGGictr:CT_243.
PATRICi20380003. VBIChlTra43535_0260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67836.1.
PIRiD71539.
RefSeqiNP_219748.1. NC_000117.1.
WP_009871590.1. NC_000117.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IU8X-ray2.20A/B/C1-354[»]
2IU9X-ray3.10A/B/C1-354[»]
2IUAX-ray2.70A/B/C1-354[»]
ProteinModelPortaliP0CD76.
SMRiP0CD76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60889N.
STRINGi272561.CT243.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC67836; AAC67836; CT_243.
GeneIDi884880.
KEGGictr:CT_243.
PATRICi20380003. VBIChlTra43535_0260.

Phylogenomic databases

eggNOGiENOG4105D7M. Bacteria.
COG1044. LUCA.
InParanoidiP0CD76.
KOiK02536.
OMAiSYPAKIM.

Enzyme and pathway databases

UniPathwayiUPA00973.
BRENDAi2.3.1.191. 1315.

Miscellaneous databases

EvolutionaryTraceiP0CD76.

Family and domain databases

CDDicd03352. LbH_LpxD. 1 hit.
HAMAPiMF_00523. LpxD. 1 hit.
InterProiIPR001451. Hexapep.
IPR007691. LpxD.
IPR011004. Trimer_LpxA-like.
IPR020573. UDP_GlcNAc_AcTrfase_non-rep.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF04613. LpxD. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01853. lipid_A_lpxD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXD_CHLTR
AccessioniPrimary (citable) accession number: P0CD76
Secondary accession number(s): O84245, Q9S530
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: February 9, 2010
Last modified: November 2, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.