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Protein

UDP-3-O-acylglucosamine N-acyltransferase

Gene

lpxD

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the N-acylation of UDP-3-O-myristoylglucosamine using 3-hydroxyarachidoyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell (Probable).1 Publication

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein].

Pathwayi: LPS lipid A biosynthesis

This protein is involved in the pathway LPS lipid A biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS lipid A biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei43 – 431Participates in a stacking interaction with the uracil ring of UDP-GlcNAc
Sitei49 – 491Participates in a stacking interaction with the uracil ring of UDP-GlcNAc
Binding sitei240 – 2401Palmitate1 Publication
Binding sitei244 – 2441Palmitate1 Publication
Active sitei247 – 2471Proton acceptor1 Publication
Binding sitei248 – 2481UDP-GlcNAc1 Publication
Binding sitei266 – 2661UDP-GlcNAc1 Publication
Binding sitei284 – 2841UDP-GlcNAc1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.191. 1315.
UniPathwayiUPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase (EC:2.3.1.-)
Gene namesi
Name:lpxD
Ordered Locus Names:CT_243
OrganismiChlamydia trachomatis (strain D/UW-3/Cx)
Taxonomic identifieri272561 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000000431 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354UDP-3-O-acylglucosamine N-acyltransferasePRO_0000059665Add
BLAST

Interactioni

Subunit structurei

Homotrimer.1 Publication

Protein-protein interaction databases

DIPiDIP-60889N.
STRINGi272561.CT243.

Structurei

Secondary structure

1
354
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 147Combined sources
Beta strandi18 – 214Combined sources
Turni33 – 353Combined sources
Beta strandi40 – 434Combined sources
Beta strandi46 – 483Combined sources
Helixi50 – 545Combined sources
Beta strandi59 – 646Combined sources
Helixi65 – 695Combined sources
Beta strandi78 – 836Combined sources
Helixi85 – 939Combined sources
Turni94 – 963Combined sources
Beta strandi117 – 1193Combined sources
Beta strandi169 – 1735Combined sources
Beta strandi184 – 1885Combined sources
Beta strandi192 – 1954Combined sources
Turni196 – 1983Combined sources
Beta strandi199 – 2024Combined sources
Beta strandi209 – 2113Combined sources
Beta strandi222 – 2254Combined sources
Beta strandi227 – 2293Combined sources
Beta strandi231 – 2333Combined sources
Beta strandi291 – 2933Combined sources
Beta strandi307 – 3104Combined sources
Turni311 – 3144Combined sources
Helixi317 – 32812Combined sources
Helixi330 – 34213Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IU8X-ray2.20A/B/C1-354[»]
2IU9X-ray3.10A/B/C1-354[»]
2IUAX-ray2.70A/B/C1-354[»]
ProteinModelPortaliP0CD76.
SMRiP0CD76. Positions 3-348.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0CD76.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni190 – 1923UDP-GlcNAc binding

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105D7M. Bacteria.
COG1044. LUCA.
InParanoidiP0CD76.
KOiK02536.
OMAiSYPAKIM.

Family and domain databases

CDDicd03352. LbH_LpxD. 1 hit.
HAMAPiMF_00523. LpxD. 1 hit.
InterProiIPR001451. Hexapep.
IPR007691. LpxD.
IPR011004. Trimer_LpxA-like.
IPR020573. UDP_GlcNAc_AcTrfase_non-rep.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF04613. LpxD. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01853. lipid_A_lpxD. 1 hit.

Sequencei

Sequence statusi: Complete.

P0CD76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSTYSLEQ LADFLKVEFQ GNGATLLSGV EEIEEAKTAH ITFLDNEKYA
60 70 80 90 100
KHLKSSEAGA IIISRTQFQK YRDLNKNFLI TSESPSLVFQ KCLELFITPV
110 120 130 140 150
DSGFPGIHPT AVIHPTAIIE DHVCIEPYAV VCQHAHVGSA CHIGSGSVIG
160 170 180 190 200
AYSTVGEHSY IHPRVVIRER VSIGKRVIIQ PGAVIGSCGF GYVTSAFGQH
210 220 230 240 250
KHLKHLGKVI IEDDVEIGAN TTIDRGRFKH SVVREGSKID NLVQIAHQVE
260 270 280 290 300
VGQHSMIVAQ AGIAGSTKIG NHVIIGGQAG ITGHICIADH VIMMAQTGVT
310 320 330 340 350
KSITSPGIYG GAPARPYQEI HRQVAKVRNL PRLEERIAAL EKLVQKLEAL

SEQH
Length:354
Mass (Da):38,404
Last modified:February 9, 2010 - v1
Checksum:iB9C547C129AE17BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67836.1.
PIRiD71539.
RefSeqiNP_219748.1. NC_000117.1.
WP_009871590.1. NC_000117.1.

Genome annotation databases

EnsemblBacteriaiAAC67836; AAC67836; CT_243.
GeneIDi884880.
KEGGictr:CT_243.
PATRICi20380003. VBIChlTra43535_0260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA. Translation: AAC67836.1.
PIRiD71539.
RefSeqiNP_219748.1. NC_000117.1.
WP_009871590.1. NC_000117.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IU8X-ray2.20A/B/C1-354[»]
2IU9X-ray3.10A/B/C1-354[»]
2IUAX-ray2.70A/B/C1-354[»]
ProteinModelPortaliP0CD76.
SMRiP0CD76. Positions 3-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60889N.
STRINGi272561.CT243.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC67836; AAC67836; CT_243.
GeneIDi884880.
KEGGictr:CT_243.
PATRICi20380003. VBIChlTra43535_0260.

Phylogenomic databases

eggNOGiENOG4105D7M. Bacteria.
COG1044. LUCA.
InParanoidiP0CD76.
KOiK02536.
OMAiSYPAKIM.

Enzyme and pathway databases

UniPathwayiUPA00973.
BRENDAi2.3.1.191. 1315.

Miscellaneous databases

EvolutionaryTraceiP0CD76.

Family and domain databases

CDDicd03352. LbH_LpxD. 1 hit.
HAMAPiMF_00523. LpxD. 1 hit.
InterProiIPR001451. Hexapep.
IPR007691. LpxD.
IPR011004. Trimer_LpxA-like.
IPR020573. UDP_GlcNAc_AcTrfase_non-rep.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF04613. LpxD. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01853. lipid_A_lpxD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXD_CHLTR
AccessioniPrimary (citable) accession number: P0CD76
Secondary accession number(s): O84245, Q9S530
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: February 9, 2010
Last modified: September 7, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.