P0CD61 (GPT_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
EC=2.7.8.15

Alternative name(s):
GlcNAc-1-P transferase
Short name=G1PT
Short name=GPT
N-acetylglucosamine-1-phosphate transferase

Gene names

Name:	alg7
ORF Names:	DDB_G0290751

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dolichol-linked oligosaccharide biosynthetic process Inferred from sequence or structural similarity. Source: dictyBase protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence or structural similarity. Source: dictyBase
Molecular_function	UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Inferred from sequence or structural similarity. Source: dictyBase phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: InterPro transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 408	408	UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase		PRO_0000391334
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Transmembrane	32 – 52	21	Helical; Potential
	Transmembrane	87 – 107	21	Helical; Potential
	Transmembrane	120 – 140	21	Helical; Potential
	Transmembrane	147 – 167	21	Helical; Potential
	Transmembrane	181 – 201	21	Helical; Potential
	Transmembrane	221 – 241	21	Helical; Potential
	Transmembrane	258 – 278	21	Helical; Potential
	Transmembrane	297 – 317	21	Helical; Potential
	Transmembrane	351 – 371	21	Helical; Potential
	Transmembrane	376 – 396	21	Helical; Potential
	Motif	95 – 107	13	Dolichol recognition
	Motif	259 – 271	13	Dolichol recognition
	Compositional bias	243 – 250	8	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	P0CD61 [UniParc]. Last modified February 9, 2010. Version 1. Checksum: 98CBC6C4BCFB5602	FASTA	408	45,452
	10 20 30 40 50 60 MIFDKSLLGF IGICCLPPIL LLIYLPNVSL QWIIVQSGFF SFGAGLLTYK LIPSVADLTS 70 80 90 100 110 120 QANLTGMDLN KKGDPKFSGK KIPESLGICV AVVYLVCVIL FQTFQWFSFP ETIQLSEYNA 130 140 150 160 170 180 ALTSICFMIL LGFGDDVLNL RWRYKLILPM FASLPLLVAY AGGTTVVVPD INFPVPLREW 190 200 210 220 230 240 LGVVFDLGIF YRIYLLMLAI FCTNSINILA GINGLEVGQS VVIATSIIIH NLIELTIASS 250 260 270 280 290 300 VPSSSSFSSS ALSSVSPHLF SLILMIPFLF TTISLLFYNW YPSRVFVGDT YTYFSGMCFA 310 320 330 340 350 360 VVAILCHFSK TLLLFFIPQI LNFLYSVPQL FGIIPCPRHR VPKFNPETGK MEAIPTNLTI 370 380 390 400 INLLLMITGP LTERQLCVYL LIFQGLCSCV GFGIRYHHLH HNPTLIFI

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000168 Genomic DNA. No translation available. AAFI02000169 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
dictyBase	DDB_G0290751. alg7.
Phylogenomic databases
OMA	YLAFVHY.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
InterPro	IPR000715. Glycosyl_transferase_4. [Graphical view]
Pfam	PF00953. Glycos_transf_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPT_DICDI

Accession

Primary (citable) accession number: P0CD61

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 9, 2010
Last sequence update:	February 9, 2010
Last modified:	October 3, 2012
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)