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P0CC17

- OXLA_BOTAT

UniProt

P0CC17 - OXLA_BOTAT

Protein

L-amino-acid oxidase

Gene
N/A
Organism
Bothrops atrox (Barba amarilla) (Fer-de-lance)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (L-Met, followed by L-Leu, L-Phe, L-Trp, L-Tyr, L-Ile), thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as apoptosis, cytotoxicity towards several cancer cell lines, induction of platelet aggregation, induction of moderate mouse paw edema, and antibacterial activities against both Gram-positive and Gram-negative bacteria. Unlike other snake venom L-amino acid oxidases, does not induce hemorrhage (with 50 µg of enzyme). May also act in hemolysis, and antiparasitic activities. Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions.2 Publications

    Catalytic activityi

    An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

    Cofactori

    FAD.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei70 – 701SubstrateBy similarity
    Binding sitei106 – 1061FADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi112 – 1176FADBy similarity
    Nucleotide bindingi112 – 1132SubstrateBy similarity

    GO - Molecular functioni

    1. L-amino-acid oxidase activity Source: UniProtKB

    GO - Biological processi

    1. apoptotic process Source: UniProtKB
    2. defense response to Gram-negative bacterium Source: UniProtKB
    3. defense response to Gram-positive bacterium Source: UniProtKB
    4. envenomation resulting in positive regulation of platelet aggregation in other organism Source: UniProtKB
    5. hemolysis in other organism Source: UniProtKB-KW
    6. modulation of apoptotic process in other organism Source: UniProtKB
    7. regulation of platelet aggregation Source: UniProtKB

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation activating toxin, Toxin

    Keywords - Biological processi

    Apoptosis, Cytolysis, Hemolysis

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-amino-acid oxidase (EC:1.4.3.2)
    Short name:
    BatroxLAAO
    Short name:
    LAAO
    Short name:
    LAO
    OrganismiBothrops atrox (Barba amarilla) (Fer-de-lance)
    Taxonomic identifieri8725 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›119›119L-amino-acid oxidasePRO_0000390927Add
    BLAST

    Post-translational modificationi

    Contains 2 disulfide bonds.By similarity
    N-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.1 Publication

    Interactioni

    Subunit structurei

    Homodimer; non-covalently linked.By similarity

    Family & Domainsi

    Sequence similaritiesi

    Sequencei

    Sequence statusi: Fragments.

    P0CC17-1 [UniParc]FASTAAdd to Basket

    « Hide

    ADDRNPLEER KFDLQLNRFS QENENAWYFI KNRVGEVNKD PGVLEYPVKP    50
    SEVGKSSAGQ LYEESLQKAH DDIFAYEKIK FEPPLPPKKF WEDDGIHGGK 100
    IYFAGETAQA HGWIDSTIK 119
    Length:119
    Mass (Da):13,700
    Last modified:January 19, 2010 - v1
    Checksum:i9006A71038ED323D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-adjacent residuesi9 – 102Curated
    Non-adjacent residuesi32 – 332Curated
    Non-adjacent residuesi69 – 702Curated
    Non-adjacent residuesi78 – 792Curated
    Non-adjacent residuesi88 – 892Curated
    Non-adjacent residuesi100 – 1012Curated
    Non-terminal residuei119 – 1191

    Cross-referencesi

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. Cited for: PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
      Tissue: Venom.
    2. Cited for: FUNCTION.
      Tissue: Venom.
    3. "A rational protocol for the successful crystallization of L-amino-acid oxidase from Bothrops atrox."
      Alves R.M., Feliciano P.R., Sampaio S.V., Nonato M.C.
      Acta Crystallogr. F 67:475-478(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION.
      Tissue: Venom.

    Entry informationi

    Entry nameiOXLA_BOTAT
    AccessioniPrimary (citable) accession number: P0CC17
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 19, 2010
    Last sequence update: January 19, 2010
    Last modified: October 1, 2014
    This is version 15 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Has parasiticidal activities against both trypanosomes and leishmania, as a result of enzyme-catalyzed hydrogen peroxide production.1 Publication

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3