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Protein

L-amino-acid oxidase

Gene
N/A
Organism
Bothrops atrox (Barba amarilla) (Fer-de-lance)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids (L-Met, followed by L-Leu, L-Phe, L-Trp, L-Tyr, L-Ile), thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as apoptosis, cytotoxicity towards several cancer cell lines, induction of platelet aggregation, induction of moderate mouse paw edema, and antibacterial activities against both Gram-positive and Gram-negative bacteria. Unlike other snake venom L-amino acid oxidases, does not induce hemorrhage (with 50 µg of enzyme). May also act in hemolysis, and antiparasitic activities. Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions.2 Publications

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701SubstrateBy similarity
Binding sitei106 – 1061FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi112 – 1176FADBy similarity
Nucleotide bindingi112 – 1132SubstrateBy similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB

GO - Biological processi

  1. apoptotic process Source: UniProtKB
  2. defense response to Gram-negative bacterium Source: UniProtKB
  3. defense response to Gram-positive bacterium Source: UniProtKB
  4. envenomation resulting in positive regulation of platelet aggregation in other organism Source: UniProtKB
  5. hemolysis in other organism Source: UniProtKB-KW
  6. modulation of apoptotic process in other organism Source: UniProtKB
  7. regulation of platelet aggregation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation activating toxin, Toxin

Keywords - Biological processi

Apoptosis, Cytolysis, Hemolysis

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase (EC:1.4.3.2)
Short name:
BatroxLAAO
Short name:
LAAO
Short name:
LAO
OrganismiBothrops atrox (Barba amarilla) (Fer-de-lance)
Taxonomic identifieri8725 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›119›119L-amino-acid oxidasePRO_0000390927Add
BLAST

Post-translational modificationi

Contains 2 disulfide bonds.By similarity
N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Interactioni

Subunit structurei

Homodimer; non-covalently linked.By similarity

Family & Domainsi

Sequence similaritiesi

Sequencei

Sequence statusi: Fragments.

P0CC17-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
ADDRNPLEER KFDLQLNRFS QENENAWYFI KNRVGEVNKD PGVLEYPVKP
60 70 80 90 100
SEVGKSSAGQ LYEESLQKAH DDIFAYEKIK FEPPLPPKKF WEDDGIHGGK
110
IYFAGETAQA HGWIDSTIK
Length:119
Mass (Da):13,700
Last modified:January 19, 2010 - v1
Checksum:i9006A71038ED323D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-adjacent residuesi9 – 102Curated
Non-adjacent residuesi32 – 332Curated
Non-adjacent residuesi69 – 702Curated
Non-adjacent residuesi78 – 792Curated
Non-adjacent residuesi88 – 892Curated
Non-adjacent residuesi100 – 1012Curated
Non-terminal residuei119 – 1191

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. Cited for: PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
    Tissue: Venom.
  2. Cited for: FUNCTION.
    Tissue: Venom.
  3. "A rational protocol for the successful crystallization of L-amino-acid oxidase from Bothrops atrox."
    Alves R.M., Feliciano P.R., Sampaio S.V., Nonato M.C.
    Acta Crystallogr. F 67:475-478(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
    Tissue: Venom.

Entry informationi

Entry nameiOXLA_BOTAT
AccessioniPrimary (citable) accession number: P0CC17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: January 19, 2010
Last modified: November 26, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Has parasiticidal activities against both trypanosomes and leishmania, as a result of enzyme-catalyzed hydrogen peroxide production.1 Publication

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.