P0CC12 (KAX85_ODODO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin alpha-KTx 8.5 Alternative name(s): OdK1
Organism	Odontobuthus doriae (Yellow Iranian scorpion)
Taxonomic identifier	342590 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Odontobuthus

Protein attributes

Sequence length	29 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Selectively inhibits voltage-gated potassium channels Kv1.2/KCNA2 with an IC50 of 183 nM. Ref.1
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed by the venom gland. Ref.1
Miscellaneous	This toxin does not affect the following channels: Kv1.1/KCNA1, Kv1.3/KCNA3, Kv1.4/KCNA4, Kv1.5/KCNA5 and Shaker IR (Ref.1).
Sequence similarities	Belongs to the short scorpion toxin superfamily.
Mass spectrometry	Molecular mass is 3180.12 Da from positions 1 - 29. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	extracellular region Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	potassium channel inhibitor activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 29	29	Potassium channel toxin alpha-KTx 8.5		PRO_0000390674
Amino acid modifications
	Disulfide bond	3 ↔ 19		By similarity
	Disulfide bond	6 ↔ 24		By similarity
	Disulfide bond	10 ↔ 26		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0CC12 [UniParc]. Last modified January 19, 2010. Version 1. Checksum: AECDB50C196DCEC8	FASTA	29	3,189
	10 20 VSCEDCPEHC STQKARAKCD NDKCVCESV

References

[1]

"The first potassium channel toxin from the venom of the Iranian scorpion Odonthobuthus doriae." Abdel-Mottaleb Y., Clynen E., Jalali A., Bosmans F., Vatanpour H., Schoofs L., Tytgat J. FEBS Lett. 580:6254-6258(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR003614. Scorpion_toxin-like. IPR008911. Toxin_6. [Graphical view]
Pfam	PF05453. Toxin_6. 1 hit. [Graphical view]
SUPFAM	SSF57095. SSF57095. 1 hit.
PROSITE	PS01138. SCORP_SHORT_TOXIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAX85_ODODO

Accession

Primary (citable) accession number: P0CC12

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 19, 2010
Last sequence update:	January 19, 2010
Last modified:	May 1, 2013
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program