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P0CB97

- NDUS8_PONAB

UniProt

P0CB97 - NDUS8_PONAB

Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

NDUFS8

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (24 Nov 2009)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi111 – 1111Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi114 – 1141Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi117 – 1171Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi121 – 1211Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi150 – 1501Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi153 – 1531Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi156 – 1561Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi160 – 1601Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. mitochondrial respiratory chain complex I assembly Source: UniProtKB
    2. response to oxidative stress Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-23kD
    Short name:
    CI-23kD
    NADH-ubiquinone oxidoreductase 23 kDa subunit
    Gene namesi
    Name:NDUFS8
    OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
    Taxonomic identifieri9601 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
    ProteomesiUP000001595: Chromosome 11

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: UniProtKB
    2. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3434MitochondrionBy similarityAdd
    BLAST
    Chaini35 – 210176NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialPRO_0000250704Add
    BLAST

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP0CB97.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini102 – 131304Fe-4S ferredoxin-type 1Add
    BLAST
    Domaini141 – 170304Fe-4S ferredoxin-type 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 23 kDa subunit family.Curated
    Contains 2 4Fe-4S ferredoxin-type domains.Curated

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    GeneTreeiENSGT00390000003049.
    KOiK03941.
    OMAiNKELSME.
    OrthoDBiEOG7J9VQT.
    TreeFamiTF105610.

    Family and domain databases

    HAMAPiMF_01351. NDH1_NuoI.
    InterProiIPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view]
    PfamiPF12838. Fer4_7. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01971. NuoI. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0CB97-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRCLTTPMLL RALAQAARAG PPCGRSLHSS AVAATYKYVN MQEPEMDMKS    50
    VTDRAARTLL LTELFRGLGM TLSYLFREPA TINYPFEKGP LSPRFRGEHA 100
    LRRYPSGEER CIACKLCEAI CPAQAITIEA EPRADGSRRT TRYDIDMTKC 150
    IYCGFCQEAC PVDAIVEGPN FEFSTETHEE LLYNKEKLLN NGDKWEAEIA 200
    ANIQADYLYR 210
    Length:210
    Mass (Da):23,692
    Last modified:November 24, 2009 - v1
    Checksum:iB4BA93CD3B59263F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR858424 mRNA. Translation: CAH90653.1.
    RefSeqiNP_001125353.1. NM_001131881.2.

    Genome annotation databases

    EnsembliENSPPYT00000003574; ENSPPYP00000003451; ENSPPYG00000002976.
    GeneIDi100172255.
    KEGGipon:100172255.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR858424 mRNA. Translation: CAH90653.1 .
    RefSeqi NP_001125353.1. NM_001131881.2.

    3D structure databases

    ProteinModelPortali P0CB97.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSPPYT00000003574 ; ENSPPYP00000003451 ; ENSPPYG00000002976 .
    GeneIDi 100172255.
    KEGGi pon:100172255.

    Organism-specific databases

    CTDi 4728.

    Phylogenomic databases

    GeneTreei ENSGT00390000003049.
    KOi K03941.
    OMAi NKELSME.
    OrthoDBi EOG7J9VQT.
    TreeFami TF105610.

    Family and domain databases

    HAMAPi MF_01351. NDH1_NuoI.
    InterProi IPR001450. 4Fe4S-bd_dom.
    IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR010226. NADH_quinone_OxRdtase_chainI.
    [Graphical view ]
    Pfami PF12838. Fer4_7. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01971. NuoI. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. The German cDNA consortium
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.

    Entry informationi

    Entry nameiNDUS8_PONAB
    AccessioniPrimary (citable) accession number: P0CB97
    Secondary accession number(s): Q0MQI1, Q5RC57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3