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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Gene

NDUFS8

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi111 – 1111Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi114 – 1141Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi117 – 1171Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi121 – 1211Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi150 – 1501Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi153 – 1531Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi156 – 1561Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi160 – 1601Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. mitochondrial respiratory chain complex I assembly Source: UniProtKB
  2. response to oxidative stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-23kD
Short name:
CI-23kD
NADH-ubiquinone oxidoreductase 23 kDa subunit
Gene namesi
Name:NDUFS8
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595 Componenti: Chromosome 11

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrial respiratory chain complex I Source: UniProtKB
  2. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434MitochondrionBy similarityAdd
BLAST
Chaini35 – 210176NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrialPRO_0000250704Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0CB97.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini102 – 131304Fe-4S ferredoxin-type 1Add
BLAST
Domaini141 – 170304Fe-4S ferredoxin-type 2Add
BLAST

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000003049.
InParanoidiP0CB97.
KOiK03941.
OMAiDLFKCIY.
OrthoDBiEOG7J9VQT.
TreeFamiTF105610.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CB97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCLTTPMLL RALAQAARAG PPCGRSLHSS AVAATYKYVN MQEPEMDMKS
60 70 80 90 100
VTDRAARTLL LTELFRGLGM TLSYLFREPA TINYPFEKGP LSPRFRGEHA
110 120 130 140 150
LRRYPSGEER CIACKLCEAI CPAQAITIEA EPRADGSRRT TRYDIDMTKC
160 170 180 190 200
IYCGFCQEAC PVDAIVEGPN FEFSTETHEE LLYNKEKLLN NGDKWEAEIA
210
ANIQADYLYR
Length:210
Mass (Da):23,692
Last modified:November 23, 2009 - v1
Checksum:iB4BA93CD3B59263F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR858424 mRNA. Translation: CAH90653.1.
RefSeqiNP_001125353.1. NM_001131881.2.
XP_009244339.1. XM_009246064.1.

Genome annotation databases

EnsembliENSPPYT00000003574; ENSPPYP00000003451; ENSPPYG00000002976.
GeneIDi100172255.
KEGGipon:100172255.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR858424 mRNA. Translation: CAH90653.1.
RefSeqiNP_001125353.1. NM_001131881.2.
XP_009244339.1. XM_009246064.1.

3D structure databases

ProteinModelPortaliP0CB97.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSPPYT00000003574; ENSPPYP00000003451; ENSPPYG00000002976.
GeneIDi100172255.
KEGGipon:100172255.

Organism-specific databases

CTDi4728.

Phylogenomic databases

GeneTreeiENSGT00390000003049.
InParanoidiP0CB97.
KOiK03941.
OMAiDLFKCIY.
OrthoDBiEOG7J9VQT.
TreeFamiTF105610.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. The German cDNA consortium
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiNDUS8_PONAB
AccessioniPrimary (citable) accession number: P0CB97
Secondary accession number(s): Q0MQI1, Q5RC57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2009
Last sequence update: November 23, 2009
Last modified: January 6, 2015
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.