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P0CB57 (DDL_STRPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Synonyms:ddlA
Ordered Locus Names:SP_1671
OrganismStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) [Complete proteome] [HAMAP]
Taxonomic identifier170187 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 347347D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177887

Regions

Domain131 – 333203ATP-grasp
Nucleotide binding161 – 21656ATP By similarity

Sites

Metal binding2871Magnesium or manganese 1 By similarity
Metal binding3001Magnesium or manganese 1 By similarity
Metal binding3001Magnesium or manganese 2 By similarity
Metal binding3021Magnesium or manganese 2 By similarity

Experimental info

Sequence conflict2501K → N in AAK75750. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P0CB57 [UniParc].

Last modified November 3, 2009. Version 1.
Checksum: AFCF7AB1E53F9289

FASTA34738,717
        10         20         30         40         50         60 
MKQTIILLYG GRSAEREVSV LSAESVMRAV DYDRFTVKTF FISQSGDFIK TQEFSHAPGQ 

        70         80         90        100        110        120 
EDRLMTNETI DWDKKVAPSA IYEEGAVVFP VLHGPMGEDG SVQGFLEVLK MPYVGCNILS 

       130        140        150        160        170        180 
SSLAMDKITT KRVLESAGIA QVPYVAIVEG DDVTAKIAEV EEKLAYPVFT KPSNMGSSVG 

       190        200        210        220        230        240 
ISKSENQEEL RQALKLAFRY DSRVLVEQGV NAREIEVGLL GNYDVKSTLP GEVVKDVAFY 

       250        260        270        280        290        300 
DYDAKYIDNK ITMDIPAKIS DDVVAVMRQN AETAFRAIGG LGLSRCDFFY TDKGEIFLNE 

       310        320        330        340 
LNTMPGFTQW SMYPLLWDNM GISYPKLIER LVDLAKESFD KREAHLI 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of a virulent isolate of Streptococcus pneumoniae."
Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D., Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J., Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D., Umayam L.A., White O., Salzberg S.L. expand/collapse author list , Lewis M.R., Radune D., Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L., McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K., Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A., Morrison D.A., Hollingshead S.K., Fraser C.M.
Science 293:498-506(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-334 / TIGR4.
[2]"Identification of three major clones of multiply antibiotic resistant Streptococcus pneumoniae in Taiwanese hospitals using multilocus sequence typing."
Zhi-Yuan S., Enright M.C., Wilkinson P., Spratt B.G.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-300.
[3]"Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae."
Coffey T.J., Enright M.C., Daniels M., Wilkinson P., Berron S., Fenoll A., Spratt B.G.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-301.
Strain: Various strains.
[4]"A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease."
Enright M.C., Spratt B.G.
Microbiology 144:3049-3060(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-301.
Strain: Various strains.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005672 Genomic DNA. Translation: AAK75750.1.
AJ233887 Genomic DNA. Translation: CAA13580.1.
Z99894 Genomic DNA. Translation: CAB17019.1.
Z99837 Genomic DNA. Translation: CAB16962.1.
Z99844 Genomic DNA. Translation: CAB16969.1.
Z99845 Genomic DNA. Translation: CAB16970.1.
Z99878 Genomic DNA. Translation: CAB17003.1.
Z99893 Genomic DNA. Translation: CAB17018.1.
AJ232272 Genomic DNA. Translation: CAA13275.1.
AJ232245 Genomic DNA. Translation: CAA13248.1.
AJ232254 Genomic DNA. Translation: CAA13257.1.
AJ232260 Genomic DNA. Translation: CAA13263.1.
AJ232267 Genomic DNA. Translation: CAA13270.1.
AJ232268 Genomic DNA. Translation: CAA13271.1.
PIRE95194.
RefSeqNP_346110.1. NC_003028.3.

3D structure databases

ProteinModelPortalP0CB57.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK75750; AAK75750; SP_1671.
GeneID931166.
KEGGspn:SP_1671.
PATRIC19707777. VBIStrPne105772_1731.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycSPNE170187:GHGN-1678-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_STRPN
AccessionPrimary (citable) accession number: P0CB57
Secondary accession number(s): O54631
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: November 3, 2009
Last modified: February 19, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways