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Protein

Peroxiredoxin-1

Gene

PRDX1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 (By similarity). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (PubMed:19766572).By similarity1 Publication

Miscellaneous

Reduced levels of PRDX1 by RNAi cause the loss of motor neurons but did not alter the number of motor neuron progenitors or the dorsal-ventral pattering of spinal progenitors, and did not compromise motor neuron survival.
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei52Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

ReactomeiR-GGA-3299685. Detoxification of Reactive Oxygen Species.
R-GGA-5628897. TP53 Regulates Metabolic Genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-1 (EC:1.11.1.15)
Gene namesi
Name:PRDX1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 8

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52C → S: Abolishes the ability to form a mixed-disulfide with GDPD5; when associated with S-173. 1 Publication1
Mutagenesisi173C → S: Abolishes the ability to form a mixed-disulfide with GDPD5; when associated with S-52. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003879611 – 199Peroxiredoxin-1Add BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52Interchain (with C-173); in linked formBy similarity
Disulfide bondi173Interchain (with C-52); in linked formBy similarity

Post-translational modificationi

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0CB50.
PRIDEiP0CB50.

Expressioni

Gene expression databases

BgeeiENSGALG00000010243.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer (By similarity). Interacts with GDPD5; forms a mixed-disulfide with GDPD5 (PubMed:19766572). Interacts with SESN1 and SESN2 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GDPD5Q3KTM25EBI-2464239,EBI-2464223

GO - Molecular functioni

Protein-protein interaction databases

IntActiP0CB50. 1 interactor.
STRINGi9031.ENSGALP00000016629.

Structurei

3D structure databases

ProteinModelPortaliP0CB50.
SMRiP0CB50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 165ThioredoxinPROSITE-ProRule annotationAdd BLAST160

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
GeneTreeiENSGT00390000004653.
HOGENOMiHOG000022343.
InParanoidiP0CB50.
KOiK13279.
OMAiFAWTNTP.
OrthoDBiEOG091G0IE5.
PhylomeDBiP0CB50.

Family and domain databases

InterProiView protein in InterPro
IPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P0CB50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGKAFIGK PAPDFTATAV MPDGQFKDIK LSDYRGKYVV FFFYPLDFTF
60 70 80 90 100
VCPTEIIAYS DRADEFKKIN CEIIGASVDS HFCHLAWINT PKKQGGLGTM
110 120 130 140 150
KIPLVSDTKR VIAKDYGVLK EDEGIAYRGL FIIDEKGILR QITINDLPVG
160 170 180 190
RSVDETLRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVQK SKEYFSKQK
Length:199
Mass (Da):22,315
Last modified:November 3, 2009 - v1
Checksum:i6377365A582E9171
GO

Sequence databases

RefSeqiNP_001258861.1. NM_001271932.1.
UniGeneiGga.5204.

Genome annotation databases

EnsembliENSGALT00000016648; ENSGALP00000016629; ENSGALG00000010243.
ENSGALT00000086986; ENSGALP00000060290; ENSGALG00000010243.
GeneIDi424598.
KEGGigga:424598.

Similar proteinsi

Entry informationi

Entry nameiPRDX1_CHICK
AccessioniPrimary (citable) accession number: P0CB50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: November 3, 2009
Last modified: August 30, 2017
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families