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Protein

Peroxiredoxin-1

Gene

PRDX1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 (By similarity). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (PubMed:19766572).By similarity1 Publication

Miscellaneous

Reduced levels of PRDX1 by RNAi cause the loss of motor neurons but did not alter the number of motor neuron progenitors or the dorsal-ventral pattering of spinal progenitors, and did not compromise motor neuron survival.
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei52Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

ReactomeiR-GGA-3299685 Detoxification of Reactive Oxygen Species
R-GGA-5628897 TP53 Regulates Metabolic Genes

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-1 (EC:1.11.1.15)
Gene namesi
Name:PRDX1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52C → S: Abolishes the ability to form a mixed-disulfide with GDPD5; when associated with S-173. 1 Publication1
Mutagenesisi173C → S: Abolishes the ability to form a mixed-disulfide with GDPD5; when associated with S-52. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003879611 – 199Peroxiredoxin-1Add BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52Interchain (with C-173); in linked formBy similarity
Disulfide bondi173Interchain (with C-52); in linked formBy similarity

Post-translational modificationi

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0CB50
PRIDEiP0CB50

Expressioni

Gene expression databases

BgeeiENSGALG00000010243

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer (By similarity). Interacts with GDPD5; forms a mixed-disulfide with GDPD5 (PubMed:19766572). Interacts with SESN1 and SESN2 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GDPD5Q3KTM25EBI-2464239,EBI-2464223

GO - Molecular functioni

Protein-protein interaction databases

IntActiP0CB50, 1 interactor
STRINGi9031.ENSGALP00000016629

Structurei

3D structure databases

ProteinModelPortaliP0CB50
SMRiP0CB50
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 165ThioredoxinPROSITE-ProRule annotationAdd BLAST160

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0852 Eukaryota
COG0450 LUCA
GeneTreeiENSGT00390000004653
HOGENOMiHOG000022343
InParanoidiP0CB50
KOiK13279
OMAiFAWTNTP
OrthoDBiEOG091G0IE5
PhylomeDBiP0CB50

Family and domain databases

InterProiView protein in InterPro
IPR000866 AhpC/TSA
IPR024706 Peroxiredoxin_AhpC-typ
IPR019479 Peroxiredoxin_C
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF10417 1-cysPrx_C, 1 hit
PF00578 AhpC-TSA, 1 hit
PIRSFiPIRSF000239 AHPC, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0CB50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGKAFIGK PAPDFTATAV MPDGQFKDIK LSDYRGKYVV FFFYPLDFTF
60 70 80 90 100
VCPTEIIAYS DRADEFKKIN CEIIGASVDS HFCHLAWINT PKKQGGLGTM
110 120 130 140 150
KIPLVSDTKR VIAKDYGVLK EDEGIAYRGL FIIDEKGILR QITINDLPVG
160 170 180 190
RSVDETLRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVQK SKEYFSKQK
Length:199
Mass (Da):22,315
Last modified:November 3, 2009 - v1
Checksum:i6377365A582E9171
GO

Sequence databases

RefSeqiNP_001258861.1, NM_001271932.1
UniGeneiGga.5204

Genome annotation databases

EnsembliENSGALT00000016648; ENSGALP00000016629; ENSGALG00000010243
ENSGALT00000086986; ENSGALP00000060290; ENSGALG00000010243
GeneIDi424598
KEGGigga:424598

Similar proteinsi

Entry informationi

Entry nameiPRDX1_CHICK
AccessioniPrimary (citable) accession number: P0CB50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: November 3, 2009
Last modified: May 23, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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