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Protein

Heat shock 70 kDa protein 1-like

Gene

HSPA1L

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Positive regulator of PARK2 translocation to damaged mitochondria.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 17ATPBy similarity4
Nucleotide bindingi204 – 206ATPBy similarity3
Nucleotide bindingi270 – 277ATPBy similarity8
Nucleotide bindingi341 – 344ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-3371571. HSF1-dependent transactivation.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock 70 kDa protein 1-like
Short name:
Heat shock 70 kDa protein 1L
Gene namesi
Name:HSPA1L
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 23

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003831151 – 641Heat shock 70 kDa protein 1-likeAdd BLAST641

Proteomic databases

PaxDbiP0CB32.
PeptideAtlasiP0CB32.
PRIDEiP0CB32.

Expressioni

Gene expression databases

BgeeiENSBTAG00000025442.

Interactioni

Subunit structurei

Interacts with PARK2.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000035635.

Structurei

3D structure databases

ProteinModelPortaliP0CB32.
SMRiP0CB32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni3 – 388Nucleotide-binding domain (NBD)By similarityAdd BLAST386
Regioni396 – 511Substrate-binding domain (SBD)By similarityAdd BLAST116

Domaini

The N-terminal nucleotide binding domain (NBD) (also known as the ATPase domain) is responsible for binding and hydrolyzing ATP. The C-terminal substrate-binding domain (SBD) (also known as peptide-binding domain) binds to the client/substrate proteins. The two domains are allosterically coupled so that, when ATP is bound to the NBD, the SBD binds relatively weakly to clients. When ADP is bound in the NBD, a conformational change enhances the affinity of the SBD for client proteins.By similarity

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
GeneTreeiENSGT00870000136409.
HOGENOMiHOG000228135.
InParanoidiP0CB32.
KOiK03283.
OMAiKELENMC.
OrthoDBiEOG091G03SF.
TreeFamiTF105042.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiView protein in InterPro
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
PfamiView protein in Pfam
PF00012. HSP70. 1 hit.
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiView protein in PROSITE
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P0CB32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAKGTAIG IDLGTTYSCV GVFQHGKVEI IANDQGNRTT PSYVAFTDTE
60 70 80 90 100
RLIGDAAKNQ VAMNPQNTVF DAKRLIGRKF NDPVVQSDMK LWPFQVINEG
110 120 130 140 150
GKPKVMVSYK GEKKAFYPEE ISSMVLTKMK ETAEAFLGYT VTNAVITVPA
160 170 180 190 200
YFNDSQRQAT KDAGVIAGLN VLRIINEPTA AAIAYGLDKA GQGERHVLIF
210 220 230 240 250
DLGGGTFDVS VLTIDDGIFE VKATAGDTHL GGEDFDNRLV SHFVEEFKRK
260 270 280 290 300
HKKDISQNKR AVRRLRTACE RAKRTLSSST QANLEIDSLY EGIDFYTSIT
310 320 330 340 350
RARFEELCAD LFRGTLEPVE KALRDAKMDK AKIHDIVLVG GSTRIPKVQR
360 370 380 390 400
LLQDYFNGRD LNKSINPDEA VAYGAAVQAA ILMGDKSEKV QDLLLLDVAP
410 420 430 440 450
LSLGLETAGG VMTVLIKRNS TIPTKQTQIF TTYSDNQPGV LIQVYEGERA
460 470 480 490 500
MTRDNNLLGR FDLTGIPPAP RGVPQIEVTF DIDANGILNV TAMDKSTGKA
510 520 530 540 550
NKITITNDKG RLSKEEIERM VLDAEKYKAE DEVQREKIAA KNALESYAFN
560 570 580 590 600
MKSAVSDEGL QGKISESDKK KILSKCNEVL LWLEANQLAE KDEFDHKRKE
610 620 630 640
LEQVCNPIIT KLYQGGCTGP SCGTGYTPGR AATGPTIEEV D
Length:641
Mass (Da):70,389
Last modified:September 1, 2009 - v1
Checksum:iB44CA29796F023ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFC03097356 Genomic DNA. No translation available.
RefSeqiNP_001161367.1. NM_001167895.1.
XP_005223735.1. XM_005223678.3.
UniGeneiBt.55003.

Genome annotation databases

EnsembliENSBTAT00000035766; ENSBTAP00000035635; ENSBTAG00000025442.
GeneIDi540190.
KEGGibta:540190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFC03097356 Genomic DNA. No translation available.
RefSeqiNP_001161367.1. NM_001167895.1.
XP_005223735.1. XM_005223678.3.
UniGeneiBt.55003.

3D structure databases

ProteinModelPortaliP0CB32.
SMRiP0CB32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000035635.

Proteomic databases

PaxDbiP0CB32.
PeptideAtlasiP0CB32.
PRIDEiP0CB32.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000035766; ENSBTAP00000035635; ENSBTAG00000025442.
GeneIDi540190.
KEGGibta:540190.

Organism-specific databases

CTDi3305.

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
GeneTreeiENSGT00870000136409.
HOGENOMiHOG000228135.
InParanoidiP0CB32.
KOiK03283.
OMAiKELENMC.
OrthoDBiEOG091G03SF.
TreeFamiTF105042.

Enzyme and pathway databases

ReactomeiR-BTA-3371571. HSF1-dependent transactivation.

Miscellaneous databases

PROiP0CB32.

Gene expression databases

BgeeiENSBTAG00000025442.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiView protein in InterPro
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
PfamiView protein in Pfam
PF00012. HSP70. 1 hit.
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiView protein in PROSITE
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHS71L_BOVIN
AccessioniPrimary (citable) accession number: P0CB32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: March 15, 2017
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.