Reviewed, UniProtKB/Swiss-Prot P0CB22 (ATX2_ARATH)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase ATX2
    EC=2.1.1.43
Alternative name(s):
    Trithorax-homolog protein 2
      Short name=TRX-homolog protein 2
    Protein SET DOMAIN GROUP 30

Gene names

Name:	ATX2
Synonyms:	SDG30, SET30
Ordered Locus Names:	At1g05830
ORF Names:	T20M3.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1083 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Histone methyltransferase By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed in roots and flowers and, to a lower extent, in young seedlings. Ref.3
Sequence similarities	Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily. Contains 2 PHD-type zinc fingers. Contains 1 post-SET domain. Contains 1 PWWP domain. Contains 1 SET domain.
Sequence caution	The sequence AAF29390.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At1g05830 and At1g05835. The sequence AK226560 differs from that shown. Reason: Frameshift at position 276.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1083	1083	Histone-lysine N-methyltransferase ATX2		PRO_0000233355
Regions
	Domain	315 – 379	65	PWWP
	Domain	918 – 1041	124	SET
	Domain	1043 – 1059	17	Post-SET
	Zinc finger	626 – 677	52	PHD-type 1
	Zinc finger	740 – 767	28	PHD-type 2; degenerate
	Compositional bias	41 – 48	8	Poly-Ser
	Compositional bias	78 – 97	20	Arg-rich

Sequences

	Sequence		Length	Mass (Da)
	P0CB22-1 [UniParc]. Last modified September 1, 2009. Version 1. Checksum: 2F773B9406BE6102	FASTA	1,083	123,287
	10 20 30 40 50 60 MISMSCVPKE EEGEDTQIKT ELHDHAADNP VRYASLESVY SVSSSSSSLC CKTAAGSHKK 70 80 90 100 110 120 VNALKLPMSD SFELQPHRRP EIVHVYCRRK RRRRRRRESF LELAILQNEG VERDDRIVKI 130 140 150 160 170 180 ESAELDDEKE EENKKKKQKK RRIGNGELMK LGVDSTTLSV SATPPLRGCR IKAVCSGNKQ 190 200 210 220 230 240 DGSSRSKRNT VKNQEKVVTA SATAKKWVRL SYDGVDPKHF IGLQCKVFWP LDAVWYPGSI 250 260 270 280 290 300 VGYNVETKHH IVKYGDGDGE ELALRREKIK FLISRDDMEL LNMKFGTNDV VVDGQDYDEL 310 320 330 340 350 360 VILAASFEEC QDFEPRDIIW AKLTGHAMWP AIIVDESVIV KRKGLNNKIS GGRSVLVQFF 370 380 390 400 410 420 GTHDFARIQV KQAVSFLKGL LSRSPLKCKQ PRFEEAMEEA KMYLKEYKLP GRMDQLQKVA 430 440 450 460 470 480 DTDCSERINS GEEDSSNSGD DYTKDGEVWL RPTELGDCLH RIGDLQIINL GRIVTDSEFF 490 500 510 520 530 540 KDSKHTWPEG YTATRKFISL KDPNASAMYK MEVLRDAESK TRPVFRVTTN SGEQFKGDTP 550 560 570 580 590 600 SACWNKIYNR IKKIQIASDN PDVLGEGLHE SGTDMFGFSN PEVDKLIQGL LQSRPPSKVS 610 620 630 640 650 660 QRKYSSGKYQ DHPTGYRPVR VEWKDLDKCN VCHMDEEYEN NLFLQCDKCR MMVHTRCYGQ 670 680 690 700 710 720 LEPHNGILWL CNLCRPVALD IPPRCCLCPV VGGAMKPTTD GRWAHLACAI WIPETCLLDV 730 740 750 760 770 780 KKMEPIDGVK KVSKDRWKLL CSICGVSYGA CIQCSNNTCR VAYHPLCARA AGLCVELADE 790 800 810 820 830 840 DRLFLLSMDD DEADQCIRLL SFCKRHRQTS NYHLETEYMI KPAHNIAEYL PPPNPSGCAR 850 860 870 880 890 900 TEPYNYLGRR GRKEPEALAG ASSKRLFVEN QPYIVGGYSR HEFSTYERIY GSKMSQITTP 910 920 930 940 950 960 SNILSMAEKY TFMKETYRKR LAFGKSGIHG FGIFAKLPHR AGDMVIEYTG ELVRPPIADK 970 980 990 1000 1010 1020 REHLIYNSMV GAGTYMFRID NERVIDATRT GSIAHLINHS CEPNCYSRVI SVNGDEHIII 1030 1040 1050 1060 1070 1080 FAKRDVAKWE ELTYDYRFFS IDERLACYCG FPRCRGVVND TEAEERQANI HASRCELKEW TES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Two Arabidopsis homologs of the animal trithorax genes: a new structural domain is a signature feature of the trithorax gene family." Alvarez-Venegas R., Avramova Z. Gene 271:215-221(2001) [PubMed: 11418242] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes." Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K., Assalkhou R., Schulz I., Reuter G., Aalen R.B. Nucleic Acids Res. 29:4319-4333(2001) [PubMed: 11691919] [Abstract] Cited for: NOMENCLATURE.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC009999 Genomic DNA. Translation: AAF29390.1. Sequence problems. AK226560 mRNA. No translation available.
IPI	IPI00519225.
PIR	A86193.
RefSeq	NP_001077464.4. NP_172074.6.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P0CB22.
Genome annotation databases
GeneID	837093.
Organism-specific databases
TAIR	At1g05830.
Enzyme and pathway databases
BRENDA	2.1.1.43. 302.
Gene expression databases
Genevestigator	P0CB22.
GermOnline	AT1G05830. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003889. FYrich_C. IPR018516. FYrich_C_sg. IPR003888. FYrich_N. IPR018518. FYrich_N_sg. IPR003616. Post-SET_Zn_bd. IPR000313. PWWP. IPR001214. SET. IPR019786. Zinc_finger_PHD-type_CS. IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. [Graphical view]
Pfam	PF05965. FYRC. 1 hit. PF05964. FYRN. 1 hit. PF00628. PHD. 2 hits. PF00855. PWWP. 1 hit. PF00856. SET. 1 hit. [Graphical view]
SMART	SM00542. FYRC. 1 hit. SM00541. FYRN. 1 hit. SM00249. PHD. 2 hits. SM00508. PostSET. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS50868. POST_SET. 1 hit. PS50812. PWWP. 1 hit. PS50280. SET. 1 hit. PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATX2_ARATH

Accession

Primary (citable) accession number: P0CB22
Secondary accession number(s): Q8RXL4, Q9MA43

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 1, 2009
Last sequence update:	September 1, 2009
Last modified:	November 24, 2009
This is version 4 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)