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Protein

Urease subunit alpha

Gene

ureC

Organism
Ureaplasma urealyticum (Ureaplasma urealyticum biotype 2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease (ureb10), Urease (ureb14), Urease subunit gamma (ureA), Urease (ureb8), Urease subunit beta (ureB), Urease (ureb9), Urease subunit alpha (ureC), Urease (ureb4), Urease (ureb6), Urease (ureb1), Urease (ureb3), Urease (ureb13)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi141 – 1411Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi143 – 1431Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi223 – 2231Nickel 1; via carbamate groupUniRule annotation
Metal bindingi223 – 2231Nickel 2; via carbamate groupUniRule annotation
Binding sitei225 – 2251SubstrateUniRule annotation
Metal bindingi252 – 2521Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi278 – 2781Nickel 2; via tele nitrogenUniRule annotation
Active sitei326 – 3261Proton donorUniRule annotation
Metal bindingi366 – 3661Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Synonyms:ureA
OrganismiUreaplasma urealyticum (Ureaplasma urealyticum biotype 2)
Taxonomic identifieri2130 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeUreaplasma

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 598598Urease subunit alphaPRO_0000067564Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei223 – 2231N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi565575.UUR10_0477.

Structurei

3D structure databases

ProteinModelPortaliP0CB00.
SMRiP0CB00. Positions 2-598.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini136 – 598463UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0CB00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKISRKNYS DLYGITTGDS VRLGDTNLWV KVEKDLTTYG EESVFGGGKT
60 70 80 90 100
LREGMGMNST MKLDDKLGNA EVMDLVITNA LILDYTGIYK ADIGIKNGKI
110 120 130 140 150
ASIGKSGNPH LTDGVDMVVG ISTEVSAGEG KIYTAGGLDT HVHWLEPEIV
160 170 180 190 200
PVALDGGITT VIAGGTGMND GTKATTVSPG KFWVKSALQA ADGLPINAGF
210 220 230 240 250
LAKGQGMEDP IFEQIVAGAC GLKIHEDWGA TGNAIDLALT VAEKTDVAVA
260 270 280 290 300
IHTDTLNEAG FVEHTIAAMK GRTIHAYHTE GAGGGHAPDI LESVKYAHIL
310 320 330 340 350
PASTNPTIPY TVNTIAEHLD MLMVCHHLNP KVPEDVAFAD SRIRSQTIAA
360 370 380 390 400
EDLLHDMGAI SIMSSDTLAM GRIGEVVTRS WQMAHKMKAQ FGALKGDSEF
410 420 430 440 450
NDNNRVKRYV AKYTINPAIA HGIDSYVGSI EVGKLADIVA WEPKFFGAKP
460 470 480 490 500
YYVVKMGVIA RCVAGDPNAS IPTCEPVIMR DQFGTYGRSL TSTSVSFVSK
510 520 530 540 550
IGLENGIKEE YKLEKELLPV KNCRSINKKS MKWNSATPNL EVDPQTFDAA
560 570 580 590
VDYNDLENWL EQPAAELAKK LKKTANGKYV LDAEPLTEAP LAQRYFLF
Length:598
Mass (Da):64,491
Last modified:July 28, 2009 - v1
Checksum:i1BF5DB65636CBDB0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291W → E AA sequence (PubMed:2050410).Curated
Sequence conflicti31 – 311K → I AA sequence (PubMed:2050410).Curated
Sequence conflicti92 – 921D → DP in CAA35697 (PubMed:2191184).Curated
Sequence conflicti144 – 1441W → R in CAA35697 (PubMed:2191184).Curated
Sequence conflicti163 – 1631A → T in CAA35697 (PubMed:2191184).Curated
Sequence conflicti181 – 1877KFWVKSA → NSELIC in CAA35697 (PubMed:2191184).Curated
Sequence conflicti231 – 2322TG → NR in CAA35697 (PubMed:2191184).Curated
Sequence conflicti371 – 3711G → E in CAA35697 (PubMed:2191184).Curated
Sequence conflicti403 – 4064NNRV → KQPC in CAA35697 (PubMed:2191184).Curated
Sequence conflicti582 – 59817DAEPL…RYFLF → ACRTSNRSSISTKILLILIL ELFWFSNSISNYI in CAA35697 (PubMed:2191184).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51315 Genomic DNA. Translation: CAA35697.1.
AF085720 Genomic DNA. Translation: AAD28106.2.
AF085721 Genomic DNA. Translation: AAD28109.2.
AF085722 Genomic DNA. Translation: AAD28112.2.
AF085723 Genomic DNA. Translation: AAD28115.2.
AF085724 Genomic DNA. Translation: AAD28118.2.
AF085725 Genomic DNA. Translation: AAD28121.2.
AF085727 Genomic DNA. Translation: AAD28127.2.
AF085728 Genomic DNA. Translation: AAD28130.2.
AF085729 Genomic DNA. Translation: AAD28133.2.
M36190 Genomic DNA. Translation: AAA79777.1.
PIRiS10032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51315 Genomic DNA. Translation: CAA35697.1.
AF085720 Genomic DNA. Translation: AAD28106.2.
AF085721 Genomic DNA. Translation: AAD28109.2.
AF085722 Genomic DNA. Translation: AAD28112.2.
AF085723 Genomic DNA. Translation: AAD28115.2.
AF085724 Genomic DNA. Translation: AAD28118.2.
AF085725 Genomic DNA. Translation: AAD28121.2.
AF085727 Genomic DNA. Translation: AAD28127.2.
AF085728 Genomic DNA. Translation: AAD28130.2.
AF085729 Genomic DNA. Translation: AAD28133.2.
M36190 Genomic DNA. Translation: AAA79777.1.
PIRiS10032.

3D structure databases

ProteinModelPortaliP0CB00.
SMRiP0CB00. Positions 2-598.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi565575.UUR10_0477.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Ureaplasma urealyticum urease genes; use of a UGA tryptophan codon."
    Blanchard A.
    Mol. Microbiol. 4:669-676(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27618 / CIP 103755 / NCTC 10177 / T960 / Serovar 8.
  2. Blanchard A.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 274-289.
  3. "Postgenomic taxonomy of human ureaplasmas - a case study based on multiple gene sequences."
    Kong F., Gilbert G.L.
    Int. J. Syst. Evol. Microbiol. 54:1815-1821(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27618 / CIP 103755 / NCTC 10177 / T960 / Serovar 8, ATCC 27814 / 23 / Serovar 2, ATCC 27816 / 58 / Serovar 4, ATCC 27817 / 354 / Serovar 5, ATCC 27819 / Co / Serovar 7, ATCC 33175 / Vancouver / Serovar 9, ATCC 33695 / K2 / Serovar 11, ATCC 33696 / U24 / Serovar 12 and ATCC 33698 / U38 / Serovar 13.
  4. "Phylogenetic analysis of Ureaplasma urealyticum -- support for the establishment of a new species, Ureaplasma parvum."
    Kong F., James G., Ma Z., Gordon S., Wang B., Gilbert G.L.
    Int. J. Syst. Bacteriol. 49:1879-1889(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-135.
    Strain: ATCC 27618 / CIP 103755 / NCTC 10177 / T960 / Serovar 8, ATCC 27814 / 23 / Serovar 2, ATCC 27816 / 58 / Serovar 4, ATCC 27817 / 354 / Serovar 5, ATCC 27819 / Co / Serovar 7, ATCC 33175 / Vancouver / Serovar 9, ATCC 33695 / K2 / Serovar 11, ATCC 33696 / U24 / Serovar 12 and ATCC 33698 / U38 / Serovar 13.
  5. "Isolation and detection of urease genes in Ureaplasma urealyticum."
    Willoughby J.J., Russell W.C., Thirkell D., Burdon M.G.
    Infect. Immun. 59:2463-2469(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-41, PROTEIN SEQUENCE OF 1-36.
    Strain: ATCC 27618 / CIP 103755 / NCTC 10177 / T960 / Serovar 8.

Entry informationi

Entry nameiURE1_UREUR
AccessioniPrimary (citable) accession number: P0CB00
Secondary accession number(s): P17272, Q56554, Q9R417
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: November 11, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.