Reviewed, UniProtKB/Swiss-Prot P0CAW5 (RNPH_CAUCR)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	CC_0152

Organism

Caulobacter crescentus (Caulobacter vibrioides) [Complete proteome] [HAMAP]

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Ribonuclease PH HAMAP MF_00564		PRO_0000139880

Sequences

	Sequence		Length	Mass (Da)
	P0CAW5-1 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: 8DC29753AD7EFE58	FASTA	238	25,549
	10 20 30 40 50 60 MRPSERAPDQ LRAVTLETGV NRYAEGSCLI GFGHTKVLVT ATVEENVPGW MRNKGAGWVT 70 80 90 100 110 120 AEYGMLPRAT HTRGRREAAA GKQTGRTQEI QRLIGRSLRA VVDLKALGER QITLDCDVVQ 130 140 150 160 170 180 ADGGTRTAAI TGAWVALRLA TKYLLDEGVL KTDPILGQVA AVSCGVFNGV PVLDLDYEED 190 200 210 220 230 SNAEADSNFV LTGVGDIVEI QATGEKRGFT RAEFESLYGL AEKGINELFA LQRAAIGG

References

[1]

"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005673 Genomic DNA. Translation: AAK22139.1.
PIR	G87267.
RefSeq	NP_418971.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	944212.
GenomeReviews	Gene locus CC_0152 in contig AE005673_GR.
KEGG	ccr:CC_0152.
TIGR	CC_0152.
Enzyme and pathway databases
BRENDA	2.7.7.56. 2191.
Family and domain databases
HAMAP	MF_00564. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR018336. Ribonuclease-PH_CS. IPR002381. RNase_PH_bac-type. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_CAUCR

Accession

Primary (citable) accession number: P0CAW5
Secondary accession number(s): P48196

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	September 22, 2009
This is version 5 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)