Reviewed, UniProtKB/Swiss-Prot P0CAR9 (PA21_CERCE)

Names and origin

Protein names	Recommended name:     Phospholipase A2 1       Short name=CC-PLA2-1     EC=3.1.1.4 Alternative name(s):     Phosphatidylcholine 2-acylhydrolase
Organism	Cerastes cerastes (Horned desert viper)
Taxonomic identifier	8697 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Viperinae › Cerastes

Protein attributes

Sequence length	50 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Non-toxic protein with a relatively high PA2 activity. Inhibits blood coagulation and platelet aggregation induced by ADP and arachidonic acid. Inhibits tumor cell adhesion and migration in a dose-dependent manner. Ref.1
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion. Ref.1
Subcellular location	Secreted.
Post-translational modification	Glycosylated (2.5%). Contains seven disulfide bonds.
Sequence similarities	Belongs to the phospholipase A2 family. Group II subfamily.
Mass spectrometry	Molecular mass is 13737.52 Da from positions 1 - 50. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›50	›50	Phospholipase A2 1		PRO_0000377503
Sites
	Active site	47	1	By similarity
	Metal binding	27	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	29	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	31	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	48	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	28 ↔ 44		By similarity
Experimental info
	Non-terminal residue	50	1

Sequences

	Sequence		Length	Mass (Da)
	P0CAR9-1 [UniParc]. Last modified June 16, 2009. Version 1. Checksum: E9D76F5CB2D13E10	FASTA	50	5,522
	10 20 30 40 50 NLYQFGKMIK HKTGKSALLS YSAYGCYCGW GGQGKPQDAT DHCCFVHDCC

References

[1]

"Two purified and characterized phospholipases A2 from Cerastes cerastes venom, that inhibit cancerous cell adhesion and migration." Zouari-Kessentini R., Luis J., Karray A., Kallech-Ziri O., Srairi-Abid N., Bazaa A., Loret E., Bezzine S., El Ayeb M., Marrakchi N. Toxicon 53:444-453(2009) Cited for: PROTEIN SEQUENCE, FUNCTION, COFACTOR, MASS SPECTROMETRY. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. Partial match. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA21_CERCE

Accession

Primary (citable) accession number: P0CAR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2009
Last sequence update:	June 16, 2009
Last modified:	October 13, 2009
This is version 4 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)