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P0CAP6 (RIR2_EBVB9) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase small chain

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase 38 kDa subunit
Ribonucleotide reductase small subunit
Gene names
ORF Names:BaRF1
OrganismEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4) [Reference proteome]
Taxonomic identifier10377 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

Binds 2 iron ions per subunit. Ref.4

Pathway

Genetic information processing; DNA replication.

Subunit structure

Heterotetramer composed of a homodimer of the large subunit BORF2 (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase small chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 302302Ribonucleoside-diphosphate reductase small chain
PRO_0000190502

Sites

Active site981 Ref.4
Metal binding611Iron 1
Metal binding911Iron 1
Metal binding911Iron 2
Metal binding941Iron 1
Metal binding1541Iron 2
Metal binding1881Iron 2
Metal binding1911Iron 2

Experimental info

Sequence conflict1221A → T no nucleotide entry Ref.4

Secondary structure

............................... 302
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0CAP6 [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: 473BF0BDFB7F0637

FASTA30234,359
        10         20         30         40         50         60 
MSKLLYVRDH EGFACLTVET HRNRWFAAHI VLTKDCGCLK LLNERDLEFY KFLFTFLAMA 

        70         80         90        100        110        120 
EKLVNFNIDE LVTSFESHDI DHYYTEQKAM ENVHGETYAN ILNMLFDGDR AAMNAYAEAI 

       130        140        150        160        170        180 
MADEALQAKI SWLRDKVAAA VTLPEKILVF LLIEGIFFIS SFYSIALLRV RGLMPGICLA 

       190        200        210        220        230        240 
NNYISRDELL HTRAASLLYN SMTAKADRPR ATWIQELFRT AVEVETAFIE ARGEGVTLVD 

       250        260        270        280        290        300 
VRAIKQFLEA TADRILGDIG QAPLYGTPPP KDCPLTYMTS IKQTNFFEQE SSDYTMLVVD 


DL 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence and expression of the B95-8 Epstein-Barr virus genome."
Baer R., Bankier A.T., Biggin M.D., Deininger P.L., Farrell P.J., Gibson T.J., Hatfull G., Hudson G.S., Satchwell S.C., Seguin C., Tuffnell P.S., Barrell B.G.
Nature 310:207-211(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Homology between two EBV early genes and HSV ribonucleotide reductase and 38K genes."
Gibson T.J., Stockwell P., Ginsburg M., Barrell B.G.
Nucleic Acids Res. 12:5087-5099(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROTEIN.
[3]"Tinkering with a viral ribonucleotide reductase."
Lembo D., Brune W.
Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[4]"Mechanistic basis for Epstein-Barr Virus ribonucleotide-reductase small-subunit function."
Schmitzberger F., Gurmu D., Dahlroth S.L., Nordlund P.
ACS Chem. Biol. 7:1764-1764(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH IRON IONS, COFACTOR, ACTIVE SITE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V01555 Genomic DNA. Translation: CAA24843.1.
AJ507799 Genomic DNA. Translation: CAD53406.1.
PIRWMBE12. A00530.
RefSeqYP_401656.1. NC_007605.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4A58X-ray1.68A/B1-302[»]
4A5CX-ray2.01A/B1-302[»]
4A5DX-ray1.79A/B1-302[»]
4A5EX-ray2.01A/B1-302[»]
4A5FX-ray2.14A/B1-302[»]
4A5HX-ray1.85A/B1-302[»]
4A5IX-ray2.05A/B1-302[»]
4A5JX-ray1.88A/B1-302[»]
ProteinModelPortalP0CAP6.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3783683.

Phylogenomic databases

ProtClustDBCLSP2509600.

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

Gene3D1.10.620.20. 1 hit.
InterProIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERPTHR23409. PTHR23409. 1 hit.
PfamPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMSSF47240. SSF47240. 1 hit.
PROSITEPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR2_EBVB9
AccessionPrimary (citable) accession number: P0CAP6
Secondary accession number(s): P03175, Q777G0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 26, 2009
Last modified: December 11, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways