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P0CAP2

- GRL1A_HUMAN

UniProt

P0CAP2 - GRL1A_HUMAN

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Protein

DNA-directed RNA polymerase II subunit GRINL1A

Gene

POLR2M

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Isoform 1 appears to be a stable component of the Pol II(G) complex form of RNA polymerase II (Pol II). Pol II synthesizes mRNA precursors and many functional non-coding RNAs and is the central component of the basal RNA polymerase II transcription machinery. Isoform 1 may play a role in the Mediator complex-dependent regulation of transcription activation. Isoform 1 acts in vitro as a negative regulator of transcriptional activation; this repression is relieved by the Mediator complex, which restores Pol II(G) activator-dependent transcription to a level equivalent to that of Pol II.1 Publication

GO - Molecular functioni

  1. DNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. maintenance of ER location Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Transcription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit GRINL1A
Alternative name(s):
DNA-directed RNA polymerase II subunit M
Glutamate receptor-like protein 1A
Gene namesi
Name:POLR2M
Synonyms:GRINL1A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:14862. POLR2M.

Subcellular locationi

Isoform 1 : Nucleus Curated

GO - Cellular componenti

  1. DNA-directed RNA polymerase II, holoenzyme Source: UniProtKB
  2. neuronal cell body Source: Ensembl
  3. nuclear envelope Source: LIFEdb
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28986.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368DNA-directed RNA polymerase II subunit GRINL1APRO_5000089546Add
BLAST

Proteomic databases

PRIDEiP0CAP2.

PTM databases

PhosphoSiteiP0CAP2.

Expressioni

Tissue specificityi

Detected in adult an fetal brain. Detected in heart, kidney, skeletal muscle, small intestine, lung, prostate and testis.1 Publication

Gene expression databases

BgeeiP0CAP2.
ExpressionAtlasiP0CAP2. baseline and differential.
GenevestigatoriP0CAP2.

Interactioni

Subunit structurei

Isoform 1 is a component of the Pol II(G) complex, which contains the RNA polymerase II (Pol II) core complex subunits and POLR2M isoform 1. Pol II(G) appears to be an abundant form of Pol II.

Protein-protein interaction databases

BioGridi123498. 51 interactions.
IntActiP0CAP2. 1 interaction.
MINTiMINT-4712385.

Structurei

3D structure databases

ProteinModelPortaliP0CAP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili301 – 33535Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the GRINL1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00530000063232.
HOVERGENiHBG098324.
InParanoidiP0CAP2.
OMAiNYCEEED.
OrthoDBiEOG70S76W.
PhylomeDBiP0CAP2.
TreeFamiTF332945.

Family and domain databases

InterProiIPR026213. GRINL1.
[Graphical view]
PRINTSiPR02085. POLR2GRINL1.

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P0CAP2-1) [UniParc]FASTAAdd to Basket

Also known as: Gdown1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCSLPRGFEP QAPEDLAQRS LVELREMLKR QERLLRNEKF ICKLPDKGKK
60 70 80 90 100
IFDSFAKLKA AIAECEEVRR KSELFNPVSL DCKLRQKAIA EVDVGTDKAQ
110 120 130 140 150
NSDPILDTSS LVPGCSSVDN IKSSQTSQNQ GLGRPTLEGD EETSEVEYTV
160 170 180 190 200
NKGPASSNRD RVPPSSEASE HHPRHRVSSQ AEDTSSSFDN LFIDRLQRIT
210 220 230 240 250
IADQGEQQSE ENASTKNLTG LSSGTEKKPH YMEVLEMRAK NPVPQLRKFK
260 270 280 290 300
TNVLPFRQND SSSHCQKSGS PISSEERRRR DKQHLDDITA ARLLPLHHMP
310 320 330 340 350
TQLLSIEESL ALQKQQKQNY EEMQAKLAAQ KLAERLNIKM RSYNPEGESS
360
GRYREVRDED DDWSSDEF
Length:368
Mass (Da):41,740
Last modified:May 26, 2009 - v1
Checksum:iB2F3ADAC21793D1E
GO
Isoform 2 (identifier: P0CAP2-2) [UniParc]FASTAAdd to Basket

Also known as: Gdown6

The sequence of this isoform differs from the canonical sequence as follows:
     39-195: Missing.

Show »
Length:211
Mass (Da):24,533
Checksum:i02CD8ED77ADC9983
GO
Isoform 3 (identifier: P0CAP2-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     323-368: MQAKLAAQKL...EDDDWSSDEF → RPFYSPQYRS...MGSLALLTKL

Show »
Length:363
Mass (Da):40,734
Checksum:i9EBE33AF873AC052
GO
Isoform 4 (identifier: Q6EEV4-1) [UniParc]FASTAAdd to Basket

Also known as: Gdown4

The sequence of this isoform can be found in the external entry Q6EEV4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:148
Mass (Da):15,131
GO
Isoform 5 (identifier: Q6EEV4-2) [UniParc]FASTAAdd to Basket

Also known as: Gdown3

The sequence of this isoform can be found in the external entry Q6EEV4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:86
Mass (Da):8,827
GO
Isoform 6 (identifier: P0CAP1-11) [UniParc]FASTAAdd to Basket

Also known as: Gcom1, GRINL1A complex locus protein 1

The sequence of this isoform can be found in the external entry P0CAP1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Based on a naturally occurring readthrough transcript which produces a MYZAP-POLR2M fusion protein.

Length:550
Mass (Da):64,046
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti171 – 1711H → D in CAB43263. (PubMed:17974005)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei39 – 195157Missing in isoform 2. 2 PublicationsVSP_037387Add
BLAST
Alternative sequencei323 – 36846MQAKL…SSDEF → RPFYSPQYRSSMNLLSLAAA AKDTRGSKSGKMGSLALLTK L in isoform 3. 1 PublicationVSP_037388Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF326773 mRNA. Translation: AAK92284.2.
AY207007 mRNA. Translation: AAO39707.1.
AY353061 mRNA. Translation: AAQ76837.1.
AK074767 mRNA. Translation: BAC11193.1.
AK074955 mRNA. Translation: BAC11313.1.
AK128618 mRNA. Translation: BAC87533.1.
AC090651 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77525.1.
BC001510 mRNA. Translation: AAH01510.1.
AL050091 mRNA. Translation: CAB43263.1.
CCDSiCCDS32252.1. [P0CAP2-1]
CCDS42045.1. [P0CAP2-2]
PIRiT08740.
RefSeqiNP_001018112.1. NM_001018102.2. [P0CAP2-2]
NP_056347.1. NM_015532.4. [P0CAP2-1]
UniGeneiHs.437256.
Hs.702937.
Hs.711270.

Genome annotation databases

EnsembliENST00000299638; ENSP00000299638; ENSG00000255529. [P0CAP2-1]
ENST00000380557; ENSP00000369930; ENSG00000255529. [P0CAP2-2]
ENST00000482852; ENSP00000432615; ENSG00000255529. [P0CAP2-3]
GeneIDi81488.
KEGGihsa:81488.
UCSCiuc002aes.3. human. [P0CAP2-2]
uc002aet.4. human. [P0CAP2-1]

Polymorphism databases

DMDMi238064986.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF326773 mRNA. Translation: AAK92284.2 .
AY207007 mRNA. Translation: AAO39707.1 .
AY353061 mRNA. Translation: AAQ76837.1 .
AK074767 mRNA. Translation: BAC11193.1 .
AK074955 mRNA. Translation: BAC11313.1 .
AK128618 mRNA. Translation: BAC87533.1 .
AC090651 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77525.1 .
BC001510 mRNA. Translation: AAH01510.1 .
AL050091 mRNA. Translation: CAB43263.1 .
CCDSi CCDS32252.1. [P0CAP2-1 ]
CCDS42045.1. [P0CAP2-2 ]
PIRi T08740.
RefSeqi NP_001018112.1. NM_001018102.2. [P0CAP2-2 ]
NP_056347.1. NM_015532.4. [P0CAP2-1 ]
UniGenei Hs.437256.
Hs.702937.
Hs.711270.

3D structure databases

ProteinModelPortali P0CAP2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 123498. 51 interactions.
IntActi P0CAP2. 1 interaction.
MINTi MINT-4712385.

PTM databases

PhosphoSitei P0CAP2.

Polymorphism databases

DMDMi 238064986.

Proteomic databases

PRIDEi P0CAP2.

Protocols and materials databases

DNASUi 81488.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000299638 ; ENSP00000299638 ; ENSG00000255529 . [P0CAP2-1 ]
ENST00000380557 ; ENSP00000369930 ; ENSG00000255529 . [P0CAP2-2 ]
ENST00000482852 ; ENSP00000432615 ; ENSG00000255529 . [P0CAP2-3 ]
GeneIDi 81488.
KEGGi hsa:81488.
UCSCi uc002aes.3. human. [P0CAP2-2 ]
uc002aet.4. human. [P0CAP2-1 ]

Organism-specific databases

CTDi 81488.
GeneCardsi GC15P057888.
HGNCi HGNC:14862. POLR2M.
MIMi 606485. gene.
neXtProti NX_P0CAP2.
PharmGKBi PA28986.
GenAtlasi Search...

Phylogenomic databases

GeneTreei ENSGT00530000063232.
HOVERGENi HBG098324.
InParanoidi P0CAP2.
OMAi NYCEEED.
OrthoDBi EOG70S76W.
PhylomeDBi P0CAP2.
TreeFami TF332945.

Miscellaneous databases

GenomeRNAii 81488.
NextBioi 71714.
PROi P0CAP2.
SOURCEi Search...

Gene expression databases

Bgeei P0CAP2.
ExpressionAtlasi P0CAP2. baseline and differential.
Genevestigatori P0CAP2.

Family and domain databases

InterProi IPR026213. GRINL1.
[Graphical view ]
PRINTSi PR02085. POLR2GRINL1.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Assignment of an ionotropic glutamate receptor-like gene (GRINL1A) to human chromosome 15q22.1 by in situ hybridization."
    Roginski R.S., Mohan Raj B.K., Finkernagel S.W., Sciorra L.J.
    Cytogenet. Cell Genet. 93:143-144(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "The human GRINL1A gene defines a complex transcription unit, an unusual form of gene organization in eukaryotes."
    Roginski R.S., Mohan Raj B.K., Birditt B., Rowen L.
    Genomics 84:265-276(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 6), TISSUE SPECIFICITY.
    Tissue: Brain and Lung.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Tissue: Heart.
  4. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain cortex.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 171-368.
    Tissue: Uterus.
  8. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
    Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
    Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
  9. Cited for: FUNCTION, RECONSTITUTION OF THE POL II(G) COMPLEX.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.

Entry informationi

Entry nameiGRL1A_HUMAN
AccessioniPrimary (citable) accession number: P0CAP2
Secondary accession number(s): Q6EER8
, Q6EES2, Q6EEV3, Q6EF00, Q6EF01, Q6EF02, Q6EF46, Q6EFN8, Q6EM48, Q6K046, Q6K050, Q6K051, Q6ZQZ3, Q8NC58, Q8NCF3, Q96DI5, Q96JB7, Q96NF5, Q9Y3V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 26, 2009
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The adjacent MYZAP and POLR2M genes are part of a complex transcription unit. The respective transcripts derive from different promoters and are alternatively spliced. In human, some transcripts of the upstream promoter of MYZAP use exons of the downstream POLR2M gene.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3