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Protein

DNA-directed RNA polymerase II subunit GRINL1A

Gene

POLR2M

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 1 appears to be a stable component of the Pol II(G) complex form of RNA polymerase II (Pol II). Pol II synthesizes mRNA precursors and many functional non-coding RNAs and is the central component of the basal RNA polymerase II transcription machinery. Isoform 1 may play a role in the Mediator complex-dependent regulation of transcription activation. Isoform 1 acts in vitro as a negative regulator of transcriptional activation; this repression is relieved by the Mediator complex, which restores Pol II(G) activator-dependent transcription to a level equivalent to that of Pol II.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciZFISH:G66-32793-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit GRINL1A
Alternative name(s):
DNA-directed RNA polymerase II subunit M
Glutamate receptor-like protein 1A
Gene namesi
Name:POLR2M
Synonyms:GRINL1A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:14862. POLR2M.

Subcellular locationi

Isoform 1 :

GO - Cellular componenti

  • DNA-directed RNA polymerase II, holoenzyme Source: UniProtKB
  • neuronal cell body Source: Ensembl
  • nuclear envelope Source: LIFEdb
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi100820829.
145781.
81488.
OpenTargetsiENSG00000255529.
PharmGKBiPA28986.

Polymorphism and mutation databases

BioMutaiPOLR2M.
DMDMi238064986.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_50000895461 – 368DNA-directed RNA polymerase II subunit GRINL1AAdd BLAST368

Proteomic databases

EPDiP0CAP2.
PaxDbiP0CAP2.
PeptideAtlasiP0CAP2.
PRIDEiP0CAP2.

PTM databases

iPTMnetiP0CAP2.
PhosphoSitePlusiP0CAP2.

Expressioni

Tissue specificityi

Detected in adult an fetal brain. Detected in heart, kidney, skeletal muscle, small intestine, lung, prostate and testis.1 Publication

Gene expression databases

BgeeiENSG00000255529.
ExpressionAtlasiP0CAP2. baseline and differential.
GenevisibleiP0CAP2. HS.

Interactioni

Subunit structurei

Isoform 1 is a component of the Pol II(G) complex, which contains the RNA polymerase II (Pol II) core complex subunits and POLR2M isoform 1. Pol II(G) appears to be an abundant form of Pol II.

Protein-protein interaction databases

BioGridi123498. 56 interactors.
DIPiDIP-61933N.
IntActiP0CAP2. 5 interactors.
MINTiMINT-4712385.
STRINGi9606.ENSP00000299638.

Structurei

3D structure databases

ProteinModelPortaliP0CAP2.
SMRiP0CAP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili301 – 335Sequence analysisAdd BLAST35

Sequence similaritiesi

Belongs to the GRINL1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IVF6. Eukaryota.
ENOG410ZKMZ. LUCA.
GeneTreeiENSGT00530000063232.
HOVERGENiHBG098324.
InParanoidiP0CAP2.
OMAiHYMTVLE.
OrthoDBiEOG091G0HQJ.
PhylomeDBiP0CAP2.
TreeFamiTF332945.

Family and domain databases

InterProiIPR026213. GRINL1.
[Graphical view]
PfamiPF15328. GCOM2. 1 hit.
[Graphical view]
PRINTSiPR02085. POLR2GRINL1.

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P0CAP2-1) [UniParc]FASTAAdd to basket
Also known as: Gdown1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MCSLPRGFEP QAPEDLAQRS LVELREMLKR QERLLRNEKF ICKLPDKGKK
60 70 80 90 100
IFDSFAKLKA AIAECEEVRR KSELFNPVSL DCKLRQKAIA EVDVGTDKAQ
110 120 130 140 150
NSDPILDTSS LVPGCSSVDN IKSSQTSQNQ GLGRPTLEGD EETSEVEYTV
160 170 180 190 200
NKGPASSNRD RVPPSSEASE HHPRHRVSSQ AEDTSSSFDN LFIDRLQRIT
210 220 230 240 250
IADQGEQQSE ENASTKNLTG LSSGTEKKPH YMEVLEMRAK NPVPQLRKFK
260 270 280 290 300
TNVLPFRQND SSSHCQKSGS PISSEERRRR DKQHLDDITA ARLLPLHHMP
310 320 330 340 350
TQLLSIEESL ALQKQQKQNY EEMQAKLAAQ KLAERLNIKM RSYNPEGESS
360
GRYREVRDED DDWSSDEF
Length:368
Mass (Da):41,740
Last modified:May 26, 2009 - v1
Checksum:iB2F3ADAC21793D1E
GO
Isoform 2 (identifier: P0CAP2-2) [UniParc]FASTAAdd to basket
Also known as: Gdown6

The sequence of this isoform differs from the canonical sequence as follows:
     39-195: Missing.

Show »
Length:211
Mass (Da):24,533
Checksum:i02CD8ED77ADC9983
GO
Isoform 3 (identifier: P0CAP2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     323-368: MQAKLAAQKL...EDDDWSSDEF → RPFYSPQYRS...MGSLALLTKL

Show »
Length:363
Mass (Da):40,734
Checksum:i9EBE33AF873AC052
GO
Isoform 4 (identifier: Q6EEV4-1) [UniParc]FASTAAdd to basket
Also known as: Gdown4
The sequence of this isoform can be found in the external entry Q6EEV4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:148
Mass (Da):15,131
GO
Isoform 5 (identifier: Q6EEV4-2) [UniParc]FASTAAdd to basket
Also known as: Gdown3
The sequence of this isoform can be found in the external entry Q6EEV4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:86
Mass (Da):8,827
GO
Isoform 6 (identifier: P0CAP1-11) [UniParc]FASTAAdd to basket
Also known as: Gcom1, GRINL1A complex locus protein 1
The sequence of this isoform can be found in the external entry P0CAP1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Based on a naturally occurring readthrough transcript which produces a MYZAP-POLR2M fusion protein.
Length:550
Mass (Da):64,046
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti171H → D in CAB43263 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03738739 – 195Missing in isoform 2. 2 PublicationsAdd BLAST157
Alternative sequenceiVSP_037388323 – 368MQAKL…SSDEF → RPFYSPQYRSSMNLLSLAAA AKDTRGSKSGKMGSLALLTK L in isoform 3. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF326773 mRNA. Translation: AAK92284.2.
AY207007 mRNA. Translation: AAO39707.1.
AY353061 mRNA. Translation: AAQ76837.1.
AK074767 mRNA. Translation: BAC11193.1.
AK074955 mRNA. Translation: BAC11313.1.
AK128618 mRNA. Translation: BAC87533.1.
AC090651 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77525.1.
BC001510 mRNA. Translation: AAH01510.1.
AL050091 mRNA. Translation: CAB43263.1.
CCDSiCCDS32252.1. [P0CAP2-1]
CCDS42045.1. [P0CAP2-2]
PIRiT08740.
RefSeqiNP_001018112.1. NM_001018102.2. [P0CAP2-2]
NP_056347.1. NM_015532.4. [P0CAP2-1]
UniGeneiHs.437256.
Hs.702937.
Hs.711270.

Genome annotation databases

EnsembliENST00000299638; ENSP00000299638; ENSG00000255529. [P0CAP2-1]
ENST00000380557; ENSP00000369930; ENSG00000255529. [P0CAP2-2]
ENST00000482852; ENSP00000432615; ENSG00000255529. [P0CAP2-3]
GeneIDi81488.
KEGGihsa:81488.
UCSCiuc002aet.6. human. [P0CAP2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF326773 mRNA. Translation: AAK92284.2.
AY207007 mRNA. Translation: AAO39707.1.
AY353061 mRNA. Translation: AAQ76837.1.
AK074767 mRNA. Translation: BAC11193.1.
AK074955 mRNA. Translation: BAC11313.1.
AK128618 mRNA. Translation: BAC87533.1.
AC090651 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77525.1.
BC001510 mRNA. Translation: AAH01510.1.
AL050091 mRNA. Translation: CAB43263.1.
CCDSiCCDS32252.1. [P0CAP2-1]
CCDS42045.1. [P0CAP2-2]
PIRiT08740.
RefSeqiNP_001018112.1. NM_001018102.2. [P0CAP2-2]
NP_056347.1. NM_015532.4. [P0CAP2-1]
UniGeneiHs.437256.
Hs.702937.
Hs.711270.

3D structure databases

ProteinModelPortaliP0CAP2.
SMRiP0CAP2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123498. 56 interactors.
DIPiDIP-61933N.
IntActiP0CAP2. 5 interactors.
MINTiMINT-4712385.
STRINGi9606.ENSP00000299638.

PTM databases

iPTMnetiP0CAP2.
PhosphoSitePlusiP0CAP2.

Polymorphism and mutation databases

BioMutaiPOLR2M.
DMDMi238064986.

Proteomic databases

EPDiP0CAP2.
PaxDbiP0CAP2.
PeptideAtlasiP0CAP2.
PRIDEiP0CAP2.

Protocols and materials databases

DNASUi81488.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000299638; ENSP00000299638; ENSG00000255529. [P0CAP2-1]
ENST00000380557; ENSP00000369930; ENSG00000255529. [P0CAP2-2]
ENST00000482852; ENSP00000432615; ENSG00000255529. [P0CAP2-3]
GeneIDi81488.
KEGGihsa:81488.
UCSCiuc002aet.6. human. [P0CAP2-1]

Organism-specific databases

CTDi81488.
DisGeNETi100820829.
145781.
81488.
GeneCardsiPOLR2M.
HGNCiHGNC:14862. POLR2M.
MIMi606485. gene.
neXtProtiNX_P0CAP2.
OpenTargetsiENSG00000255529.
PharmGKBiPA28986.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IVF6. Eukaryota.
ENOG410ZKMZ. LUCA.
GeneTreeiENSGT00530000063232.
HOVERGENiHBG098324.
InParanoidiP0CAP2.
OMAiHYMTVLE.
OrthoDBiEOG091G0HQJ.
PhylomeDBiP0CAP2.
TreeFamiTF332945.

Enzyme and pathway databases

BioCyciZFISH:G66-32793-MONOMER.

Miscellaneous databases

GenomeRNAii81488.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000255529.
ExpressionAtlasiP0CAP2. baseline and differential.
GenevisibleiP0CAP2. HS.

Family and domain databases

InterProiIPR026213. GRINL1.
[Graphical view]
PfamiPF15328. GCOM2. 1 hit.
[Graphical view]
PRINTSiPR02085. POLR2GRINL1.
ProtoNetiSearch...

Entry informationi

Entry nameiGRL1A_HUMAN
AccessioniPrimary (citable) accession number: P0CAP2
Secondary accession number(s): Q6EER8
, Q6EES2, Q6EEV3, Q6EF00, Q6EF01, Q6EF02, Q6EF46, Q6EFN8, Q6EM48, Q6K046, Q6K050, Q6K051, Q6ZQZ3, Q8NC58, Q8NCF3, Q96DI5, Q96JB7, Q96NF5, Q9Y3V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 26, 2009
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The adjacent MYZAP and POLR2M genes are part of a complex transcription unit. The respective transcripts derive from different promoters and are alternatively spliced. In human, some transcripts of the upstream promoter of MYZAP use exons of the downstream POLR2M gene.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.