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P0CAP2 (GRL1A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase II subunit GRINL1A
Alternative name(s):
DNA-directed RNA polymerase II subunit M
Glutamate receptor-like protein 1A
Gene names
Name:POLR2M
Synonyms:GRINL1A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Isoform 1 appears to be a stable component of the Pol II(G) complex form of RNA polymerase II (Pol II). Pol II synthesizes mRNA precursors and many functional non-coding RNAs and is the central component of the basal RNA polymerase II transcription machinery. Isoform 1 may play a role in the Mediator complex-dependent regulation of transcription activation. Isoform 1 acts in vitro as a negative regulator of transcriptional activation; this repression is relieved by the Mediator complex, which restores Pol II(G) activator-dependent transcription to a level equivalent to that of Pol II. Ref.9

Subunit structure

Isoform 1 is a component of the Pol II(G) complex, which contains the RNA polymerase II (Pol II) core complex subunits and POLR2M isoform 1. Pol II(G) appears to be an abundant form of Pol II.

Subcellular location

Isoform 1: Nucleus Probable.

Tissue specificity

Detected in adult an fetal brain. Detected in heart, kidney, skeletal muscle, small intestine, lung, prostate and testis. Ref.2

Miscellaneous

The adjacent MYZAP and POLR2M genes are part of a complex transcription unit. The respective transcripts derive from different promoters and are alternatively spliced. In human, some transcripts of the upstream promoter of MYZAP use exons of the downstream POLR2M gene.

Sequence similarities

Belongs to the GRINL1 family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
Nucleus
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmaintenance of ER location

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentDNA-directed RNA polymerase II, holoenzyme

Inferred from direct assay PubMed 22231121. Source: UniProtKB

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

nuclear envelope

Inferred from direct assay. Source: LIFEdb

   Molecular_functionDNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P0CAP2-1)

Also known as: Gdown1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P0CAP2-2)

Also known as: Gdown6;

The sequence of this isoform differs from the canonical sequence as follows:
     39-195: Missing.
Isoform 3 (identifier: P0CAP2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     323-368: MQAKLAAQKL...EDDDWSSDEF → RPFYSPQYRS...MGSLALLTKL
Isoform 4 (identifier: Q6EEV4-1)

Also known as: Gdown4;

The sequence of this isoform can be found in the external entry Q6EEV4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform 5 (identifier: Q6EEV4-2)

Also known as: Gdown3;

The sequence of this isoform can be found in the external entry Q6EEV4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform 6 (identifier: P0CAP1-11)

Also known as: Gcom1; GRINL1A complex locus protein 1;

The sequence of this isoform can be found in the external entry P0CAP1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Based on a naturally occurring readthrough transcript which produces a MYZAP-POLR2M fusion protein.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 368368DNA-directed RNA polymerase II subunit GRINL1A
PRO_5000089546

Regions

Coiled coil301 – 33535 Potential

Natural variations

Alternative sequence39 – 195157Missing in isoform 2.
VSP_037387
Alternative sequence323 – 36846MQAKL…SSDEF → RPFYSPQYRSSMNLLSLAAA AKDTRGSKSGKMGSLALLTK L in isoform 3.
VSP_037388

Experimental info

Sequence conflict1711H → D in CAB43263. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Gdown1) [UniParc].

Last modified May 26, 2009. Version 1.
Checksum: B2F3ADAC21793D1E

FASTA36841,740
        10         20         30         40         50         60 
MCSLPRGFEP QAPEDLAQRS LVELREMLKR QERLLRNEKF ICKLPDKGKK IFDSFAKLKA 

        70         80         90        100        110        120 
AIAECEEVRR KSELFNPVSL DCKLRQKAIA EVDVGTDKAQ NSDPILDTSS LVPGCSSVDN 

       130        140        150        160        170        180 
IKSSQTSQNQ GLGRPTLEGD EETSEVEYTV NKGPASSNRD RVPPSSEASE HHPRHRVSSQ 

       190        200        210        220        230        240 
AEDTSSSFDN LFIDRLQRIT IADQGEQQSE ENASTKNLTG LSSGTEKKPH YMEVLEMRAK 

       250        260        270        280        290        300 
NPVPQLRKFK TNVLPFRQND SSSHCQKSGS PISSEERRRR DKQHLDDITA ARLLPLHHMP 

       310        320        330        340        350        360 
TQLLSIEESL ALQKQQKQNY EEMQAKLAAQ KLAERLNIKM RSYNPEGESS GRYREVRDED 


DDWSSDEF 

« Hide

Isoform 2 (Gdown6) [UniParc].

Checksum: 02CD8ED77ADC9983
Show »

FASTA21124,533
Isoform 3 [UniParc].

Checksum: 9EBE33AF873AC052
Show »

FASTA36340,734
Isoform 4 (Gdown4) [UniParc].

See Q6EEV4.

Isoform 5 (Gdown3) [UniParc].

See Q6EEV4.

Isoform 6 (Gcom1) (GRINL1A complex locus protein 1) [UniParc].

See P0CAP1.

References

« Hide 'large scale' references
[1]"Assignment of an ionotropic glutamate receptor-like gene (GRINL1A) to human chromosome 15q22.1 by in situ hybridization."
Roginski R.S., Mohan Raj B.K., Finkernagel S.W., Sciorra L.J.
Cytogenet. Cell Genet. 93:143-144(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"The human GRINL1A gene defines a complex transcription unit, an unusual form of gene organization in eukaryotes."
Roginski R.S., Mohan Raj B.K., Birditt B., Rowen L.
Genomics 84:265-276(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 6), TISSUE SPECIFICITY.
Tissue: Brain and Lung.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Heart.
[4]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain cortex.
[7]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 171-368.
Tissue: Uterus.
[8]"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[9]"A Mediator-responsive form of metazoan RNA polymerase II."
Hu X., Malik S., Negroiu C.C., Hubbard K., Velalar C.N., Hampton B., Grosu D., Catalano J., Roeder R.G., Gnatt A.
Proc. Natl. Acad. Sci. U.S.A. 103:9506-9511(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, RECONSTITUTION OF THE POL II(G) COMPLEX.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF326773 mRNA. Translation: AAK92284.2.
AY207007 mRNA. Translation: AAO39707.1.
AY353061 mRNA. Translation: AAQ76837.1.
AK074767 mRNA. Translation: BAC11193.1.
AK074955 mRNA. Translation: BAC11313.1.
AK128618 mRNA. Translation: BAC87533.1.
AC090651 Genomic DNA. No translation available.
CH471082 Genomic DNA. Translation: EAW77525.1.
BC001510 mRNA. Translation: AAH01510.1.
AL050091 mRNA. Translation: CAB43263.1.
PIRT08740.
RefSeqNP_001018112.1. NM_001018102.2.
NP_056347.1. NM_015532.4.
UniGeneHs.437256.
Hs.702937.
Hs.711270.

3D structure databases

ProteinModelPortalP0CAP2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid123498. 19 interactions.
MINTMINT-4712385.

PTM databases

PhosphoSiteP0CAP2.

Polymorphism databases

DMDM238064986.

Proteomic databases

PRIDEP0CAP2.

Protocols and materials databases

DNASU81488.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000299638; ENSP00000299638; ENSG00000255529. [P0CAP2-1]
ENST00000380557; ENSP00000369930; ENSG00000255529. [P0CAP2-2]
ENST00000380563; ENSP00000369937; ENSG00000255529. [P0CAP2-3]
ENST00000482852; ENSP00000432615; ENSG00000255529. [P0CAP2-3]
GeneID81488.
KEGGhsa:81488.
UCSCuc002aes.3. human. [P0CAP2-2]
uc002aet.4. human. [P0CAP2-1]

Organism-specific databases

CTD81488.
GeneCardsGC15P057888.
HGNCHGNC:14862. POLR2M.
MIM606485. gene.
neXtProtNX_P0CAP2.
PharmGKBPA28986.
GenAtlasSearch...

Phylogenomic databases

HOVERGENHBG098324.
OMAMEESLAI.
OrthoDBEOG70S76W.
PhylomeDBP0CAP2.
TreeFamTF332945.

Gene expression databases

ArrayExpressP0CAP2.
BgeeP0CAP2.
GenevestigatorP0CAP2.

Family and domain databases

InterProIPR026213. GRINL1.
[Graphical view]
PRINTSPR02085. POLR2GRINL1.
ProtoNetSearch...

Other

GenomeRNAi81488.
NextBio71714.
PROP0CAP2.
SOURCESearch...

Entry information

Entry nameGRL1A_HUMAN
AccessionPrimary (citable) accession number: P0CAP2
Secondary accession number(s): Q6EER8 expand/collapse secondary AC list , Q6EES2, Q6EEV3, Q6EF00, Q6EF01, Q6EF02, Q6EF46, Q6EFN8, Q6EM48, Q6K046, Q6K050, Q6K051, Q6ZQZ3, Q8NC58, Q8NCF3, Q96DI5, Q96JB7, Q96NF5, Q9Y3V6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: May 26, 2009
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM