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P0CAP2

- GRL1A_HUMAN

UniProt

P0CAP2 - GRL1A_HUMAN

Protein

DNA-directed RNA polymerase II subunit GRINL1A

Gene

POLR2M

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (26 May 2009)
      Previous versions | rss
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    Functioni

    Isoform 1 appears to be a stable component of the Pol II(G) complex form of RNA polymerase II (Pol II). Pol II synthesizes mRNA precursors and many functional non-coding RNAs and is the central component of the basal RNA polymerase II transcription machinery. Isoform 1 may play a role in the Mediator complex-dependent regulation of transcription activation. Isoform 1 acts in vitro as a negative regulator of transcriptional activation; this repression is relieved by the Mediator complex, which restores Pol II(G) activator-dependent transcription to a level equivalent to that of Pol II.1 Publication

    GO - Molecular functioni

    1. DNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. maintenance of ER location Source: Ensembl

    Keywords - Biological processi

    Transcription

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase II subunit GRINL1A
    Alternative name(s):
    DNA-directed RNA polymerase II subunit M
    Glutamate receptor-like protein 1A
    Gene namesi
    Name:POLR2M
    Synonyms:GRINL1A
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:14862. POLR2M.

    Subcellular locationi

    Isoform 1 : Nucleus Curated

    GO - Cellular componenti

    1. DNA-directed RNA polymerase II, holoenzyme Source: UniProtKB
    2. neuronal cell body Source: Ensembl
    3. nuclear envelope Source: LIFEdb

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28986.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 368368DNA-directed RNA polymerase II subunit GRINL1APRO_5000089546Add
    BLAST

    Proteomic databases

    PRIDEiP0CAP2.

    PTM databases

    PhosphoSiteiP0CAP2.

    Expressioni

    Tissue specificityi

    Detected in adult an fetal brain. Detected in heart, kidney, skeletal muscle, small intestine, lung, prostate and testis.1 Publication

    Gene expression databases

    ArrayExpressiP0CAP2.
    BgeeiP0CAP2.
    GenevestigatoriP0CAP2.

    Interactioni

    Subunit structurei

    Isoform 1 is a component of the Pol II(G) complex, which contains the RNA polymerase II (Pol II) core complex subunits and POLR2M isoform 1. Pol II(G) appears to be an abundant form of Pol II.

    Protein-protein interaction databases

    BioGridi123498. 20 interactions.
    IntActiP0CAP2. 1 interaction.
    MINTiMINT-4712385.

    Structurei

    3D structure databases

    ProteinModelPortaliP0CAP2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili301 – 33535Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GRINL1 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    HOVERGENiHBG098324.
    OMAiNYCEEED.
    OrthoDBiEOG70S76W.
    PhylomeDBiP0CAP2.
    TreeFamiTF332945.

    Family and domain databases

    InterProiIPR026213. GRINL1.
    [Graphical view]
    PRINTSiPR02085. POLR2GRINL1.

    Sequences (6)i

    Sequence statusi: Complete.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: P0CAP2-1) [UniParc]FASTAAdd to Basket

    Also known as: Gdown1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MCSLPRGFEP QAPEDLAQRS LVELREMLKR QERLLRNEKF ICKLPDKGKK    50
    IFDSFAKLKA AIAECEEVRR KSELFNPVSL DCKLRQKAIA EVDVGTDKAQ 100
    NSDPILDTSS LVPGCSSVDN IKSSQTSQNQ GLGRPTLEGD EETSEVEYTV 150
    NKGPASSNRD RVPPSSEASE HHPRHRVSSQ AEDTSSSFDN LFIDRLQRIT 200
    IADQGEQQSE ENASTKNLTG LSSGTEKKPH YMEVLEMRAK NPVPQLRKFK 250
    TNVLPFRQND SSSHCQKSGS PISSEERRRR DKQHLDDITA ARLLPLHHMP 300
    TQLLSIEESL ALQKQQKQNY EEMQAKLAAQ KLAERLNIKM RSYNPEGESS 350
    GRYREVRDED DDWSSDEF 368
    Length:368
    Mass (Da):41,740
    Last modified:May 26, 2009 - v1
    Checksum:iB2F3ADAC21793D1E
    GO
    Isoform 2 (identifier: P0CAP2-2) [UniParc]FASTAAdd to Basket

    Also known as: Gdown6

    The sequence of this isoform differs from the canonical sequence as follows:
         39-195: Missing.

    Show »
    Length:211
    Mass (Da):24,533
    Checksum:i02CD8ED77ADC9983
    GO
    Isoform 3 (identifier: P0CAP2-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         323-368: MQAKLAAQKL...EDDDWSSDEF → RPFYSPQYRS...MGSLALLTKL

    Show »
    Length:363
    Mass (Da):40,734
    Checksum:i9EBE33AF873AC052
    GO
    Isoform 4 (identifier: Q6EEV4-1) [UniParc]FASTAAdd to Basket

    Also known as: Gdown4

    The sequence of this isoform can be found in the external entry Q6EEV4.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:148
    Mass (Da):15,131
    GO
    Isoform 5 (identifier: Q6EEV4-2) [UniParc]FASTAAdd to Basket

    Also known as: Gdown3

    The sequence of this isoform can be found in the external entry Q6EEV4.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:86
    Mass (Da):8,827
    GO
    Isoform 6 (identifier: P0CAP1-11) [UniParc]FASTAAdd to Basket

    Also known as: Gcom1, GRINL1A complex locus protein 1

    The sequence of this isoform can be found in the external entry P0CAP1.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Based on a naturally occurring readthrough transcript which produces a MYZAP-POLR2M fusion protein.

    Length:550
    Mass (Da):64,046
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti171 – 1711H → D in CAB43263. (PubMed:17974005)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei39 – 195157Missing in isoform 2. 2 PublicationsVSP_037387Add
    BLAST
    Alternative sequencei323 – 36846MQAKL…SSDEF → RPFYSPQYRSSMNLLSLAAA AKDTRGSKSGKMGSLALLTK L in isoform 3. 1 PublicationVSP_037388Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF326773 mRNA. Translation: AAK92284.2.
    AY207007 mRNA. Translation: AAO39707.1.
    AY353061 mRNA. Translation: AAQ76837.1.
    AK074767 mRNA. Translation: BAC11193.1.
    AK074955 mRNA. Translation: BAC11313.1.
    AK128618 mRNA. Translation: BAC87533.1.
    AC090651 Genomic DNA. No translation available.
    CH471082 Genomic DNA. Translation: EAW77525.1.
    BC001510 mRNA. Translation: AAH01510.1.
    AL050091 mRNA. Translation: CAB43263.1.
    CCDSiCCDS32252.1. [P0CAP2-1]
    CCDS42045.1. [P0CAP2-2]
    PIRiT08740.
    RefSeqiNP_001018112.1. NM_001018102.2. [P0CAP2-2]
    NP_056347.1. NM_015532.4. [P0CAP2-1]
    UniGeneiHs.437256.
    Hs.702937.
    Hs.711270.

    Genome annotation databases

    EnsembliENST00000299638; ENSP00000299638; ENSG00000255529. [P0CAP2-1]
    ENST00000380557; ENSP00000369930; ENSG00000255529. [P0CAP2-2]
    ENST00000482852; ENSP00000432615; ENSG00000255529. [P0CAP2-3]
    GeneIDi81488.
    KEGGihsa:81488.
    UCSCiuc002aes.3. human. [P0CAP2-2]
    uc002aet.4. human. [P0CAP2-1]

    Polymorphism databases

    DMDMi238064986.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF326773 mRNA. Translation: AAK92284.2 .
    AY207007 mRNA. Translation: AAO39707.1 .
    AY353061 mRNA. Translation: AAQ76837.1 .
    AK074767 mRNA. Translation: BAC11193.1 .
    AK074955 mRNA. Translation: BAC11313.1 .
    AK128618 mRNA. Translation: BAC87533.1 .
    AC090651 Genomic DNA. No translation available.
    CH471082 Genomic DNA. Translation: EAW77525.1 .
    BC001510 mRNA. Translation: AAH01510.1 .
    AL050091 mRNA. Translation: CAB43263.1 .
    CCDSi CCDS32252.1. [P0CAP2-1 ]
    CCDS42045.1. [P0CAP2-2 ]
    PIRi T08740.
    RefSeqi NP_001018112.1. NM_001018102.2. [P0CAP2-2 ]
    NP_056347.1. NM_015532.4. [P0CAP2-1 ]
    UniGenei Hs.437256.
    Hs.702937.
    Hs.711270.

    3D structure databases

    ProteinModelPortali P0CAP2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 123498. 20 interactions.
    IntActi P0CAP2. 1 interaction.
    MINTi MINT-4712385.

    PTM databases

    PhosphoSitei P0CAP2.

    Polymorphism databases

    DMDMi 238064986.

    Proteomic databases

    PRIDEi P0CAP2.

    Protocols and materials databases

    DNASUi 81488.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000299638 ; ENSP00000299638 ; ENSG00000255529 . [P0CAP2-1 ]
    ENST00000380557 ; ENSP00000369930 ; ENSG00000255529 . [P0CAP2-2 ]
    ENST00000482852 ; ENSP00000432615 ; ENSG00000255529 . [P0CAP2-3 ]
    GeneIDi 81488.
    KEGGi hsa:81488.
    UCSCi uc002aes.3. human. [P0CAP2-2 ]
    uc002aet.4. human. [P0CAP2-1 ]

    Organism-specific databases

    CTDi 81488.
    GeneCardsi GC15P057888.
    HGNCi HGNC:14862. POLR2M.
    MIMi 606485. gene.
    neXtProti NX_P0CAP2.
    PharmGKBi PA28986.
    GenAtlasi Search...

    Phylogenomic databases

    HOVERGENi HBG098324.
    OMAi NYCEEED.
    OrthoDBi EOG70S76W.
    PhylomeDBi P0CAP2.
    TreeFami TF332945.

    Miscellaneous databases

    GenomeRNAii 81488.
    NextBioi 71714.
    PROi P0CAP2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P0CAP2.
    Bgeei P0CAP2.
    Genevestigatori P0CAP2.

    Family and domain databases

    InterProi IPR026213. GRINL1.
    [Graphical view ]
    PRINTSi PR02085. POLR2GRINL1.
    ProtoNeti Search...

    Publicationsi

    1. "Assignment of an ionotropic glutamate receptor-like gene (GRINL1A) to human chromosome 15q22.1 by in situ hybridization."
      Roginski R.S., Mohan Raj B.K., Finkernagel S.W., Sciorra L.J.
      Cytogenet. Cell Genet. 93:143-144(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Brain.
    2. "The human GRINL1A gene defines a complex transcription unit, an unusual form of gene organization in eukaryotes."
      Roginski R.S., Mohan Raj B.K., Birditt B., Rowen L.
      Genomics 84:265-276(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 6), TISSUE SPECIFICITY.
      Tissue: Brain and Lung.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
      Tissue: Heart.
    4. "Analysis of the DNA sequence and duplication history of human chromosome 15."
      Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
      , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
      Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain cortex.
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 171-368.
      Tissue: Uterus.
    8. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."
      Sato S., Tomomori-Sato C., Parmely T.J., Florens L., Zybailov B., Swanson S.K., Banks C.A.S., Jin J., Cai Y., Washburn M.P., Conaway J.W., Conaway R.C.
      Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    9. Cited for: FUNCTION, RECONSTITUTION OF THE POL II(G) COMPLEX.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.

    Entry informationi

    Entry nameiGRL1A_HUMAN
    AccessioniPrimary (citable) accession number: P0CAP2
    Secondary accession number(s): Q6EER8
    , Q6EES2, Q6EEV3, Q6EF00, Q6EF01, Q6EF02, Q6EF46, Q6EFN8, Q6EM48, Q6K046, Q6K050, Q6K051, Q6ZQZ3, Q8NC58, Q8NCF3, Q96DI5, Q96JB7, Q96NF5, Q9Y3V6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    The adjacent MYZAP and POLR2M genes are part of a complex transcription unit. The respective transcripts derive from different promoters and are alternatively spliced. In human, some transcripts of the upstream promoter of MYZAP use exons of the downstream POLR2M gene.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3