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Protein

Polyprotein pp220

Gene

Pret-104

Organism
African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) (ASFV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Polyprotein pp220 is essential for the core assembly. Its myristoyl moiety may function as a membrane-anchoring signal to bind the developing core shell to the inner viral envelope (By similarity).By similarity
The structural protein p34 is a component of the virus core shell.By similarity
The structural protein p14 is a component of the virus core shell.By similarity
The structural protein p37 is a component of the virus core shell.By similarity
The structural protein p150 is a component of the virus core shell.By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprotein pp220
Alternative name(s):
220 kDa polyprotein
Cleaved into the following 4 chains:
Gene namesi
Ordered Locus Names:Pret-104
OrganismiAfrican swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) (ASFV)
Taxonomic identifieri561443 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000000859 Componenti: Genome

Subcellular locationi

Polyprotein pp220 :
p34 :
  • Virion Curated
  • Host cytoplasmhost perinuclear region By similarity

  • Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope (By similarity).By similarity
p14 :
  • Virion Curated
  • Host cytoplasmhost perinuclear region By similarity

  • Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope (By similarity).By similarity
p37 :
  • Virion Curated
  • Host cytoplasmhost perinuclear region By similarity
  • Host nucleus By similarity

  • Note: Nuclear at early stages of infection. Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope (By similarity).By similarity
p150 :
  • Virion Curated
  • Host cytoplasmhost perinuclear region By similarity

  • Note: Found in perinuclear cytoplasmic viral factories during assembly. In the virion, located in the core shell, which functions like a matrix between the DNA-containing nucleoid and the inner envelope (By similarity).By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
PropeptideiPRO_00003734332 – 45By similarityAdd BLAST44
ChainiPRO_000037343446 – 894Polyprotein pp220Sequence analysisAdd BLAST849
ChainiPRO_000037343546 – 369p34Sequence analysisAdd BLAST324
ChainiPRO_0000373436370 – 523p14Sequence analysisAdd BLAST154
ChainiPRO_0000373437524 – 894p37Sequence analysisAdd BLAST371
ChainiPRO_0000373438895 – 2475p150Sequence analysisAdd BLAST1581

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostBy similarity1

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei45 – 46Cleavage; by viral protease S273RSequence analysis2
Sitei369 – 370Cleavage; by viral protease S273RSequence analysis2
Sitei523 – 524Cleavage; by viral protease S273RSequence analysis2
Sitei894 – 895Cleavage; by viral protease S273RSequence analysis2

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PRIDEiP0CA03.

Expressioni

Inductioni

Expressed late in the infection.By similarity

Keywords - Developmental stagei

Late protein

Structurei

3D structure databases

SMRiP0CA03.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili2184 – 2211Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi551 – 554Poly-Tyr4

Sequence similaritiesi

Belongs to the asfivirus polyprotein pp220 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

OrthoDBiVOG09000009.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0CA03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNRGSSTSS RPPPSSEANI YAKLQDHIQR QTRPFSGGGY FNGGGDKNPV
60 70 80 90 100
QHIKDYHIDS VSSKAKLRII EGIIRAIAKI GFKVDTKQPI EDILKDIKKQ
110 120 130 140 150
LPDPRAGSTF VKNAEKQETV CKMIADAINQ EFIDLGQDKL IDTTEGAASI
160 170 180 190 200
CRQIVLYINS LTHGLRAEYL DVHGSIENTL ENIKLLNDAI KQLHERMVTE
210 220 230 240 250
VTKAAPNEEV INAVTMIEAV YRRLLNEQNL QINILTNFID NILTPTQKEL
260 270 280 290 300
DKLQTDEVDI IKLLNDTNSV LGTKNFGKVL SYTLCNLGIA ASVANKINKA
310 320 330 340 350
LQKVGLKVEQ YLQSKNWEEF DKELDLKRFS GLVSAENIAE FEKAVNLLRQ
360 370 380 390 400
TFNERHKILE NSCAKKGGDE EKTPLDRRIE AQRLDRKHIL MEFLNKSTQA
410 420 430 440 450
YNDFLENVKK IGIKLVKEIA LTPNITRLRD ALSRINDMGT IALDLSLIGF
460 470 480 490 500
YTNAAAREER ETFLTQFMLV KNVLEEQSKT DPNFKNLYDS CSRLLQIIDF
510 520 530 540 550
YTDIVQKKYG GEEDCECTRV GGAALTVEEL GLSKAARSQV DLNQAINTFM
560 570 580 590 600
YYYYVAQIYS NLTHNKQEFQ SYEENYATIL GDAIAGRLMQ LDTEKNARIN
610 620 630 640 650
SPAVDLARGH VGPNPGGAQE VDWKAAVSAI ELEYDVKRRF YRALEGLDLY
660 670 680 690 700
LKNITKTFVN NIDSIQTVQQ MLDGVRIIGR WFTEATGDTL AQVFESFPTS
710 720 730 740 750
AGNDSNVFTD NAPAGHYYEK VAAEIQQGRS VGTLRPVRAS QAKNIRDLIG
760 770 780 790 800
RSLSNFQALK NIINAFARIG DMLGGEELRQ MVPMSPLQIY KTLLEYIQHS
810 820 830 840 850
ALSVGLKNLN QSEIGGQRVA LARTPEEAAQ RVYLSTVRVN DALSTRWETE
860 870 880 890 900
DVFFTFMLKS MAAKIFIVLG IYDMFERPEP VYKLIPTRMI LGGADELEPE
910 920 930 940 950
VIPEAAELYF RLPRLAEFYQ KLFSFRDENV QISMLPELEG IFSGLIRIIF
960 970 980 990 1000
MRPIELINIG DYSETEIRQL IKEINVIYQH FNLEYGEQEA TKKALIHFVN
1010 1020 1030 1040 1050
EINRRFGVIT RTEWEKFQRI VQEARTMNDF GMMNQTNYSI LPDEDGYTQS
1060 1070 1080 1090 1100
SQLLPSDRFI SPSSQPTPKW RPALYNIDSV DVQTGMLQPN SQWDLVQKFR
1110 1120 1130 1140 1150
KQLSEMFEDP SLQQELGKVS YQELIRQAIN ELKKDHTDKI QIVSKLIQGS
1160 1170 1180 1190 1200
ESLADTDVNK IFLFHETVIT GLNLLSAIYV LLNNFRNNIK GLDLDTIQKS
1210 1220 1230 1240 1250
IIEWLRETPA NVNHANLIDW LGRKHGAISE IRNPGLVIKE INMRLSEVYP
1260 1270 1280 1290 1300
DPTTEANVPQ DRNLTTETLF AWIVPYVGIP AGGGVRAEQE LAARYLVDNQ
1310 1320 1330 1340 1350
RIMQLLLTNI FEMTSSFNKM VQVRFPETST AQVHLDFTGL ISLIDSLMAD
1360 1370 1380 1390 1400
TKYFLNLLRP HIDKNIIQYY ENRSNPGSFY WLEEHLIDKL IKPPTDAGGR
1410 1420 1430 1440 1450
PLPGGELGLE GVNQIINKTY TLLTKPYNVL QLRGGAQRRD AANIQINNNP
1460 1470 1480 1490 1500
QPSERFEQYG RVFSRLVFYD ALENNSGLRV EQVVLGDFRL SNLIRTNNAQ
1510 1520 1530 1540 1550
EENALSYWDN IALRTYANVN DAANNLRRYR LYGSDHGIQN NRSMMMVFNQ
1560 1570 1580 1590 1600
LVASYIARFY DAPSGKIYLN LINAFANGNF SQAVMEMGYA HPDLARNNNA
1610 1620 1630 1640 1650
FGHRGDPTEQ SVLLLSLGLI LQRLIKDTNR QGLSQHLIST LTEIPIYLKE
1660 1670 1680 1690 1700
NYRANLPLFN KMFNILISQG ELLKQFIQYT NVQLARPNLT ALLGANNDSV
1710 1720 1730 1740 1750
IYYNNNINVP MTGLSVGQAA MRGIGGVFRP NVTLMPLGDA QSNTSDIVRK
1760 1770 1780 1790 1800
RLVAVIDGII RGSHTLADSA MEVLHELTDH PIYLETEEHF IQNYMSRYNK
1810 1820 1830 1840 1850
EPLMPFSLSL YYLRDLRIEN NEVYDPLLYP NLESGSPEFK LLYGTRKLLG
1860 1870 1880 1890 1900
NDPVQLSDMP GVQLIMKNYN ETVVAREQIT PTRFEHFYTH AIQALRFIIN
1910 1920 1930 1940 1950
IRSFKTVMMY NENTFGGVNL ISENRDDKPI ITAGIGMNAV YSLRKTLQDV
1960 1970 1980 1990 2000
ISFVESSYQE EQINHIHKIV SPKGQTRTLG SNRERERIFN LFDMNIIPIN
2010 2020 2030 2040 2050
VNALMRSIPL ANIYNYDYSF EEIACLMYGI SAEKVRSLNT AAPQPDIAEV
2060 2070 2080 2090 2100
LNIPNRPPMN TREFMLKLLI NPYVSVSITQ YGNELLSKGN AGYMSRIFRG
2110 2120 2130 2140 2150
DNALNMGRPK FLSDQIFNKV LFGSLYPTQF DYDEAGPSLA AGIQRGRERW
2160 2170 2180 2190 2200
GHPMSIYINQ ALHEIVRTIR LAETVRGLRN VIDRNQIIGE LNAFRTQLED
2210 2220 2230 2240 2250
TRREVNNLIQ TPEIRNNPTP EIIAAVQNWG QQYRGQITDL IDLIGNVGQA
2260 2270 2280 2290 2300
NSMINLIQNI TPQTAGAQLI ALFNIRGLPA PPPRQVIQND IEAMQWFMTM
2310 2320 2330 2340 2350
VINHPPILIA PFMLLVNNLK EFLNTLERYV YKTPRWLGPG TARIAQPPVG
2360 2370 2380 2390 2400
MAPGINMRHH TSYTENSVLT YITEQNREEG PWSIVKQVGV GIQKPTLVQI
2410 2420 2430 2440 2450
GKDRFDTRLI RNLIFITNIQ RLLRLRLNLE LSQFRNVLVS PDHIINPSIT
2460 2470
EYGFSITGPS ETFSDKQYDS DIRIL
Length:2,475
Mass (Da):281,269
Last modified:May 5, 2009 - v1
Checksum:iE2F3F89B41C3F702
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY261363 Genomic DNA. No translation available.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPP220_ASFP4
AccessioniPrimary (citable) accession number: P0CA03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: May 10, 2017
This is version 17 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families