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P0C9E2 (TPRT_ASFK5) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Trans-prenyltransferase
EC=2.5.1.-
Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Dimethylallyltranstransferase
EC=2.5.1.1
Farnesyl diphosphate synthase
Farnesyltranstransferase
EC=2.5.1.29
Geranyltranstransferase
EC=2.5.1.10
Polyprenyl-diphosphate synthase
Gene names
Ordered Locus Names:Ken-086
OrganismAfrican swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV) [Complete proteome]
Taxonomic identifier561445 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus
Virus hostOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Trans-prenyltransferase that catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with different allylic diphosphates, such as dimethylallyl diphosphate (DMAPP), geranyl diphosphate (GPP), farnesyl diphosphate (FPP) and geranylgeranyl diphosphate (GGPP), farnesyl diphosphate being the best allylic substrate By similarity.

Catalytic activity

Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate.

Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.

(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.

Geranylgeranyl diphosphate + isopentenyl diphosphate = diphosphate + geranylfarnesyl diphosphate.

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Pathway

Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.

Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.

Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1.

Subcellular location

Host endoplasmic reticulum. Host membrane; Single-pass membrane protein By similarity.

Induction

Expressed late in the infection cycle By similarity.

Sequence similarities

Belongs to the FPP/GGPP synthase family. Asfivirus trans-prenyltransferase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Trans-prenyltransferase
PRO_0000373160

Regions

Transmembrane1 – 2121Helical; Potential

Sites

Metal binding1291Magnesium 1 By similarity
Metal binding1291Magnesium 2 By similarity
Metal binding1351Magnesium 1 By similarity
Metal binding1351Magnesium 2 By similarity
Metal binding2571Magnesium 3 By similarity
Binding site851Isopentenyl diphosphate By similarity
Binding site881Isopentenyl diphosphate By similarity
Binding site1221Isopentenyl diphosphate By similarity
Binding site1401Dimethylallyl diphosphate By similarity
Binding site1411Isopentenyl diphosphate By similarity
Binding site2161Dimethylallyl diphosphate By similarity
Binding site2171Dimethylallyl diphosphate By similarity
Binding site2541Dimethylallyl diphosphate By similarity

Sequences

Sequence LengthMass (Da)Tools
P0C9E2 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: 5FC389014B9AE803

FASTA31835,856
        10         20         30         40         50         60 
MLHLIYISII VVLIIILISY TRKPQYFRIT APRSVALFHG IHPLNPKNYK TFSEEFETIL 

        70         80         90        100        110        120 
NNAIEDGDFK GQLTEPCSYA LRGGKYIRPI ILMEIVRACQ LQHSFGAPIY PAEAALAVEY 

       130        140        150        160        170        180 
FHVASLIIDD MPSFDNDVKR RNKDTVWARF GVAKAQMSAL ALTMQGFQNI CRQVDWIKEN 

       190        200        210        220        230        240 
CPRFPDPNQL GALLCTFVSH SLNSAGSGQL VDTPEKTIPF FKIAFIMGWV LGTGTIEDIG 

       250        260        270        280        290        300 
AIERAAHCFG NAFQLADDIK DHDTDTGRNY AKIHGKRKTF DVVAQSLQEC KKILHEKKIY 

       310 
TSIWNEIFQK VINVALGT

« Hide

References

[1]"African swine fever virus genomes."
Kutish G.F., Rock D.L.
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY261360 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortalP0C9E2.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
IPR008949. Terpenoid_synth.
[Graphical view]
Gene3DG3DSA:1.10.600.10. Terpenoid_synth. 2 hits.
PANTHERPTHR12001. Polyprenyl_synt. 1 hit.
PfamPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
SUPFAMSSF48576. Terpenoid_synth. 1 hit.
PROSITEPS00723. POLYPRENYL_SYNTHASE_1. False negative.
PS00444. POLYPRENYL_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTPRT_ASFK5
AccessionPrimary (citable) accession number: P0C9E2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: September 21, 2011
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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