Reviewed, UniProtKB/Swiss-Prot P0C9E1 (TPRT_ASFWA)

Names and origin

Protein names

Recommended name:
    Trans-prenyltransferase
    EC=2.5.1.-
Alternative name(s):
    Polyprenyl-diphosphate synthase
    Dimethylallyltranstransferase
    EC=2.5.1.1
    Geranyltranstransferase
    EC=2.5.1.10
    Farnesyltranstransferase
    EC=2.5.1.29

Gene names

Ordered Locus Names:

War-084

Organism

African swine fever virus (isolate Warthog/Namibia/Wart80/1980) (ASFV)

Taxonomic identifier

561444 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Trans-prenyltransferase that catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with different allylic diphosphates, such as dimethylallyl diphosphate (DMAPP), geranyl diphosphate (GPP), farnesyl diphosphate (FPP) and geranylgeranyl diphosphate (GGPP), farnesyl diphosphate being the best allylic substrate By similarity.
Catalytic activity	Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate. Trans,trans-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate. Geranylgeranyl diphosphate + isopentenyl diphosphate = diphosphate + geranylfarnesyl diphosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1. Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1. Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1.
Subcellular location	Host endoplasmic reticulum. Host membrane; Single-pass membrane protein By similarity.
Induction	Expressed late in the infection cycle By similarity.
Sequence similarities	Belongs to the FPP/GGPP synthetase family. Asfivirus trans-prenyltransferase subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Host endoplasmic reticulum Host membrane Membrane
Developmental stage	Late protein
Domain	Transmembrane
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	host cell endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dimethylallyltranstransferase activity Inferred from electronic annotation. Source: EC farnesyltranstransferase activity Inferred from electronic annotation. Source: EC geranyltranstransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Trans-prenyltransferase		PRO_0000373159
Regions
	Transmembrane	1 – 21	21	Potential
Sites
	Metal binding	129	1	Magnesium 1 By similarity
	Metal binding	129	1	Magnesium 2 By similarity
	Metal binding	135	1	Magnesium 1 By similarity
	Metal binding	135	1	Magnesium 2 By similarity
	Metal binding	257	1	Magnesium 3 By similarity
	Binding site	85	1	Isopentenyl diphosphate By similarity
	Binding site	88	1	Isopentenyl diphosphate By similarity
	Binding site	122	1	Isopentenyl diphosphate By similarity
	Binding site	140	1	Dimethylallyl diphosphate By similarity
	Binding site	141	1	Isopentenyl diphosphate By similarity
	Binding site	216	1	Dimethylallyl diphosphate By similarity
	Binding site	217	1	Dimethylallyl diphosphate By similarity
	Binding site	254	1	Dimethylallyl diphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C9E1-1 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 7AED549204C2A6AC	FASTA	318	35,959
	10 20 30 40 50 60 MLHLIYISII VVLIIILISY TRKPKYFRIT APRSVALFHG IHPLNPKNYK TFSEEFETIL 70 80 90 100 110 120 NNAIEDGDFK GQLTEPCSYA LRGGKYIRPI ILMEIVRACQ LQHSFGAPIY PAEAALAVEY 130 140 150 160 170 180 FHVASLIIDD MPSFDNDVKR RNKDTVWARF GVAKAQMSAL ALTMQGFQNI CRQIDWIKEH 190 200 210 220 230 240 CPRFPDPNQL GALLCTFVSH SLNSAGSGQL VDTPEKTIPF FKIAFIMGWV LGTGSVEDIG 250 260 270 280 290 300 MIERAAHCFG NAFQLADDIK DHDTDTGWNY AKIHGKRKTF DDVAQFLQEC KKILHGKKIY 310 TSIWNEIFQK VINVALGT

References

[1]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY261366 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
PROSITE	PS00723. POLYPRENYL_SYNTHET_1. False negative. PS00444. POLYPRENYL_SYNTHET_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPRT_ASFWA

Accession

Primary (citable) accession number: P0C9E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	January 19, 2010
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)