Reviewed, UniProtKB/Swiss-Prot P0C992 (DNLI_ASFK5)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Ordered Locus Names:

Ken-112

Organism

African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV)

Taxonomic identifier

561445 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Very low-fidelity DNA ligase that seals, during DNA repair, nicks in double-stranded DNA. Together with the viral repair DNA polymerase X, fills the single nucleotide gaps generated by the AP endonuclease. It is not essential for viral replication and recombination By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Miscellaneous	Consistent with its intracellular location, ASFV encodes its own replicative DNA polymerase and three base excision repair enzymes: a class II AP endonuclease, the repair polymerase Pol X, and an ATP-dependent DNA ligase.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	DNA ligase		PRO_0000373091
Sites
	Active site	151	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C992-1 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: EF69CD1F30D21BF8	FASTA	419	48,147
	10 20 30 40 50 60 MLNQFPGQLS NNVFCFPPIE SETKSGKKAS WIICVQVMHH NTILPITDEM FSTDVKDAVA 70 80 90 100 110 120 EIFTKFFVEE GAVRISKTTR VTEGKNLGKK NATTVVHQAF KDALSKYNRH ARQKRGAHTN 130 140 150 160 170 180 RGMIPPMLVK HFNIIPKTFF EDETDPIVQR KRNGVRAVAC QQGDGSILLY SRTEKEFLGL 190 200 210 220 230 240 DNIKKELKQL YLFIDVRVYL DGELYLHHKP LQWIAGQANA KVDSSELHFY VFDCFWSDQL 250 260 270 280 290 300 QMPSNKRQQL LTNIFKQKED LIFIHQVENF SIKDEDEALR LKTQFIKEGY EGAIVRNANG 310 320 330 340 350 360 PYEPGYNNYH SPHLAKLKPL LDAEFILVDY TQGKKGKDLG AILWVCELPN KKRFVVTPKH 370 380 390 400 410 LTYADRYALF QKLTPALFKK HLYGKELTVE YAELSPKTGI PLQARAVGFR EPINVLEII

References

[1]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY261360 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Pfam	PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_ASFK5

Accession

Primary (citable) accession number: P0C992

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	November 24, 2009
This is version 3 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)