P0C989 (RPB1_ASFK5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable DNA-directed RNA polymerase subunit 1 homolog
EC=2.7.7.6

Gene names

Ordered Locus Names:

Ken-111

Organism

African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV) [Complete proteome]

Taxonomic identifier

561445 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	1450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the DNA-dependent RNA polymerase that catalyzes the transcription in the cytoplasm of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase that consists of at least 6 subunits Probable.
Domain	Lacks the typical C-terminal domain (CTD).
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1450	1450	Probable DNA-directed RNA polymerase subunit 1 homolog		PRO_0000373088

Sequences

	Sequence		Length	Mass (Da)
	P0C989 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 456314BF07D01250	FASTA	1,450	163,832
	10 20 30 40 50 60 MEAGYAEIAA VQFNIAGDND HKRQGVMEVT ISNLFEGTLP AEGGIYDARM GTTDHHYKCI 70 80 90 100 110 120 TCSHQRKQCM GHPGILQMHA PVLQPLFIAE IRRWLRVICL NCGAPIVDLK RYEHLIRPKR 130 140 150 160 170 180 LAEAASSQTE GKQCYVCKVI HPKIIKDSED YFTFWVDQQG KIDKLYPQII REIFSRVTYD 190 200 210 220 230 240 TVVKLGRSKN SHPEKLVLKA IQIPPISIRP GIRLGIGSGP QSFHDINNVI QYIVRKNLLI 250 260 270 280 290 300 PKDLQIVRGQ KIPLNVDRNL QTIQQLYYNF LLDSVSTTAT QGGTGKRGIV MGARPAPSIM 310 320 330 340 350 360 RRLPRKEGRI RKSLLGSQVW SISRSTICGN SDLHLDEVGY PISFARTLQV AETVQHYNIN 370 380 390 400 410 420 RLMPYFLNGK RQYPGCSRVY KQITQSVHDI EGLKQDFRLE VGDILYRDVV TGDVAFFNRQ 430 440 450 460 470 480 PSLERSSIGV HRIVVLENPK ISTFQMNVSA CAWYNADFDG DQMNLWVPWS VMSRVEAELL 490 500 510 520 530 540 CSVRNWFIST KSSGPVNGQV QDSTVGSFLL TRTNTPMGKN VMNKLHAMGL FQTTQTDPPC 550 560 570 580 590 600 FANYSPTDLL DGKLVVSMLL KQTPINYQRA PTWYSEVYAP YMHYNKQDIS TQIRNGELIE 610 620 630 640 650 660 GVLDKKAVGA GSSGGIYHLI SRRYGPQQAL KMIFATQQLA LNYVRNAGFT VSTADMLLTP 670 680 690 700 710 720 EAHQEVQEII NELLLESEEI NNRLLHGDIM PPIGLTTHDF YEKLQLNALK FPDRILKPIM 730 740 750 760 770 780 NSINPETNGL FQMVATGAKG SNPNMIHIMA GIGQIEINTQ RIQPQFSFGR TLVYYPRFAL 790 800 810 820 830 840 EAQAYGFICN SYIAGLTSPE FIFGEMNGRF DLINKALSTS STGYANRKAI FGLQSCIVDY 850 860 870 880 890 900 YRRVSIDTRL VQQLYGEDGL DARQLETVRF ETIMLSDQEL EDKFKYTGIQ SPLFEEEFSR 910 920 930 940 950 960 LKKDRDKYRQ IFLNIENFNF SQLLTDVRQV PVNVASIVKN ILLSSTSGVL PFDEKSILQK 970 980 990 1000 1010 1020 YTMVKTFCKN LPYVFINNIQ ERLQTPIPVY LKRAASLMRI LIRIELATVK TLNITCEQMS 1030 1040 1050 1060 1070 1080 AILDLIRLQY TQSLINYGEA VGILAAQSVS EPLTQYMLDS HHRSVAGGTN KSGIVRPQEI 1090 1100 1110 1120 1130 1140 FSAKPVEAEQ SSEMLLRLKN PEVETNKTYA QEIANSIELI TFERLILQWH LLYETYSSTK 1150 1160 1170 1180 1190 1200 KNVMYPDFAS DVEWMTDFLE NHPLLQPPED IANWCIRLEL NKTTMILKSI SLESIVNSLR 1210 1220 1230 1240 1250 1260 AKHPNTYIMH SVENTASGIP IIIRIYLRES AFRRSTNTRM ATDEKIAVNL VDKLLNSTIR 1270 1280 1290 1300 1310 1320 GIPGIKNANV VKLMRHRVDA QGKLVRLDNI YAIKTNGTNI FGAMLDDNID PYTIVSSSIG 1330 1340 1350 1360 1370 1380 DTMELYGIEA ARQKIISEIR TVMGDKGPNH RHLLMYADLM TRTGQVTSLE KAGLNAREPS 1390 1400 1410 1420 1430 1440 NVLLRMALSS PVQVLTDAAI DSAVNPIYGI AAPTLMGSVP RIGTMYSDII MDEKYITENY 1450 KSVDSMIDML

References

[1]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY261360 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. IPR007073. RNA_pol_Rpb1_7. [Graphical view]
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. PF04990. RNA_pol_Rpb1_7. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPB1_ASFK5

Accession

Primary (citable) accession number: P0C989

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	January 11, 2011
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program