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Protein

Endo-type membrane-bound lytic murein transglycosylase A

Gene

emtA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Preferentially cleaves at a distance of more than two disaccharide units from the ends of the glycan chain. Prefers cross-linked murein in vivo.

Catalytic activityi

Endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation

GO - Molecular functioni

  • carbon-oxygen lyase activity, acting on polysaccharides Source: InterPro
  • lytic endotransglycosylase activity Source: EcoCyc
  • lytic transglycosylase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:G6622-MONOMER.
MetaCyc:G6622-MONOMER.
BRENDAi4.2.2.B6. 2026.

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-type membrane-bound lytic murein transglycosylase AUniRule annotation (EC:4.2.2.n2UniRule annotation)
Alternative name(s):
Peptidoglycan lytic endotransglycosylaseUniRule annotation
Gene namesi
Name:emtAUniRule annotation
Synonyms:mltE, sltZ, ycgP
Ordered Locus Names:b1193, JW5821
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13897. emtA.

Subcellular locationi

  • Cell outer membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15UniRule annotationAdd BLAST15
ChainiPRO_000019656816 – 203Endo-type membrane-bound lytic murein transglycosylase AAdd BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi16N-palmitoyl cysteine1 Publication1
Lipidationi16S-diacylglycerol cysteineUniRule annotation1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP0C960.
PRIDEiP0C960.

Interactioni

Protein-protein interaction databases

BioGridi4259642. 360 interactors.
IntActiP0C960. 2 interactors.
STRINGi316385.ECDH10B_1246.

Structurei

Secondary structure

1203
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 37Combined sources8
Helixi40 – 50Combined sources11
Helixi54 – 65Combined sources12
Turni79 – 82Combined sources4
Turni85 – 87Combined sources3
Helixi88 – 96Combined sources9
Helixi104 – 108Combined sources5
Helixi110 – 126Combined sources17
Turni127 – 131Combined sources5
Helixi135 – 148Combined sources14
Helixi150 – 155Combined sources6
Helixi161 – 168Combined sources8
Helixi173 – 183Combined sources11
Helixi188 – 202Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y8PX-ray2.00A/B19-203[»]
3T36X-ray2.25A/B/C/D/E17-203[»]
4HJVX-ray2.30A/B/C/D/E17-203[»]
4HJYX-ray2.40A/B17-203[»]
4HJZX-ray1.90A/B17-203[»]
ProteinModelPortaliP0C960.
SMRiP0C960.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transglycosylase Slt family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4106S48. Bacteria.
COG0741. LUCA.
HOGENOMiHOG000219318.
KOiK08308.

Family and domain databases

HAMAPiMF_01381. EmtA. 1 hit.
InterProiView protein in InterPro
IPR023946. EmtA.
IPR023346. Lysozyme-like_dom.
IPR000189. Transglyc_AS.
IPR008258. Transglycosylase_SLT_dom_1.
PfamiView protein in Pfam
PF01464. SLT. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiView protein in PROSITE
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS00922. TRANSGLYCOSYLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLRWFAFLI VLLAGCSSKH DYTNPPWNAK VPVQRAMQWM PISQKAGAAW
60 70 80 90 100
GVDPQLITAI IAIESGGNPN AVSKSNAIGL MQLKASTSGR DVYRRMGWSG
110 120 130 140 150
EPTTSELKNP ERNISMGAAY LNILETGPLA GIEDPKVLQY ALVVSYANGA
160 170 180 190 200
GALLRTFSSD RKKAISKIND LDADEFLEHV ARNHPAPQAP RYIYKLEQAL

DAM
Length:203
Mass (Da):22,227
Last modified:May 5, 2009 - v1
Checksum:iA511D1432D8C03DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74277.2.
AP009048 Genomic DNA. Translation: BAA36051.2.
PIRiF64865.
RefSeqiNP_415711.2. NC_000913.3.
WP_001301104.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74277; AAC74277; b1193.
BAA36051; BAA36051; BAA36051.
GeneIDi945655.
KEGGiecj:JW5821.
eco:b1193.
PATRICifig|1411691.4.peg.1093.

Similar proteinsi

Entry informationi

Entry nameiEMTA_ECOLI
AccessioniPrimary (citable) accession number: P0C960
Secondary accession number(s): O87500, P76009, P94775
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: August 30, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families