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Protein

Endo-type membrane-bound lytic murein transglycosylase A

Gene

emtA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Preferentially cleaves at a distance of more than two disaccharide units from the ends of the glycan chain. Prefers cross-linked murein in vivo.

Catalytic activityi

Endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:G6622-MONOMER.
MetaCyc:G6622-MONOMER.
BRENDAi4.2.2.B6. 2026.

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-type membrane-bound lytic murein transglycosylase AUniRule annotation (EC:4.2.2.n2UniRule annotation)
Alternative name(s):
Peptidoglycan lytic endotransglycosylaseUniRule annotation
Gene namesi
Name:emtAUniRule annotation
Synonyms:mltE, sltZ, ycgP
Ordered Locus Names:b1193, JW5821
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13897. emtA.

Subcellular locationi

  • Cell outer membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15UniRule annotationAdd BLAST15
ChainiPRO_000019656816 – 203Endo-type membrane-bound lytic murein transglycosylase AAdd BLAST188

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi16N-palmitoyl cysteine1 Publication1
Lipidationi16S-diacylglycerol cysteineUniRule annotation1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP0C960.
PRIDEiP0C960.

Interactioni

Protein-protein interaction databases

BioGridi4259642. 360 interactors.
IntActiP0C960. 2 interactors.
STRINGi511145.b1193.

Structurei

Secondary structure

1203
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 37Combined sources8
Helixi40 – 50Combined sources11
Helixi54 – 65Combined sources12
Turni79 – 82Combined sources4
Turni85 – 87Combined sources3
Helixi88 – 96Combined sources9
Helixi104 – 108Combined sources5
Helixi110 – 126Combined sources17
Turni127 – 131Combined sources5
Helixi135 – 148Combined sources14
Helixi150 – 155Combined sources6
Helixi161 – 168Combined sources8
Helixi173 – 183Combined sources11
Helixi188 – 202Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y8PX-ray2.00A/B19-203[»]
3T36X-ray2.25A/B/C/D/E17-203[»]
4HJVX-ray2.30A/B/C/D/E17-203[»]
4HJYX-ray2.40A/B17-203[»]
4HJZX-ray1.90A/B17-203[»]
ProteinModelPortaliP0C960.
SMRiP0C960.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transglycosylase Slt family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4106S48. Bacteria.
COG0741. LUCA.
HOGENOMiHOG000219318.
KOiK08308.
OMAiPQAPRYL.

Family and domain databases

HAMAPiMF_01381. EmtA. 1 hit.
InterProiIPR023946. EmtA.
IPR023346. Lysozyme-like_dom.
IPR000189. Transglyc_AS.
IPR008258. Transglycosylase_SLT_dom_1.
[Graphical view]
PfamiPF01464. SLT. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS00922. TRANSGLYCOSYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLRWFAFLI VLLAGCSSKH DYTNPPWNAK VPVQRAMQWM PISQKAGAAW
60 70 80 90 100
GVDPQLITAI IAIESGGNPN AVSKSNAIGL MQLKASTSGR DVYRRMGWSG
110 120 130 140 150
EPTTSELKNP ERNISMGAAY LNILETGPLA GIEDPKVLQY ALVVSYANGA
160 170 180 190 200
GALLRTFSSD RKKAISKIND LDADEFLEHV ARNHPAPQAP RYIYKLEQAL

DAM
Length:203
Mass (Da):22,227
Last modified:May 5, 2009 - v1
Checksum:iA511D1432D8C03DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74277.2.
AP009048 Genomic DNA. Translation: BAA36051.2.
PIRiF64865.
RefSeqiNP_415711.2. NC_000913.3.
WP_001301104.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74277; AAC74277; b1193.
BAA36051; BAA36051; BAA36051.
GeneIDi945655.
KEGGiecj:JW5821.
eco:b1193.
PATRICi32117634. VBIEscCol129921_1239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74277.2.
AP009048 Genomic DNA. Translation: BAA36051.2.
PIRiF64865.
RefSeqiNP_415711.2. NC_000913.3.
WP_001301104.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y8PX-ray2.00A/B19-203[»]
3T36X-ray2.25A/B/C/D/E17-203[»]
4HJVX-ray2.30A/B/C/D/E17-203[»]
4HJYX-ray2.40A/B17-203[»]
4HJZX-ray1.90A/B17-203[»]
ProteinModelPortaliP0C960.
SMRiP0C960.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259642. 360 interactors.
IntActiP0C960. 2 interactors.
STRINGi511145.b1193.

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.

Proteomic databases

PaxDbiP0C960.
PRIDEiP0C960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74277; AAC74277; b1193.
BAA36051; BAA36051; BAA36051.
GeneIDi945655.
KEGGiecj:JW5821.
eco:b1193.
PATRICi32117634. VBIEscCol129921_1239.

Organism-specific databases

EchoBASEiEB3656.
EcoGeneiEG13897. emtA.

Phylogenomic databases

eggNOGiENOG4106S48. Bacteria.
COG0741. LUCA.
HOGENOMiHOG000219318.
KOiK08308.
OMAiPQAPRYL.

Enzyme and pathway databases

BioCyciEcoCyc:G6622-MONOMER.
MetaCyc:G6622-MONOMER.
BRENDAi4.2.2.B6. 2026.

Miscellaneous databases

PROiP0C960.

Family and domain databases

HAMAPiMF_01381. EmtA. 1 hit.
InterProiIPR023946. EmtA.
IPR023346. Lysozyme-like_dom.
IPR000189. Transglyc_AS.
IPR008258. Transglycosylase_SLT_dom_1.
[Graphical view]
PfamiPF01464. SLT. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS00922. TRANSGLYCOSYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEMTA_ECOLI
AccessioniPrimary (citable) accession number: P0C960
Secondary accession number(s): O87500, P76009, P94775
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.