P0C956 (GEL4_ASPFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,3-beta-glucanosyltransferase gel4
EC=2.4.1.-

Alternative name(s):
Glucan elongating glucanosyltransferase 4

Gene names

Name:	gel4
ORF Names:	AFUA_2G05340

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)

Taxonomic identifier

330879 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in cell wall morphogenesis By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Post-translational modification	The GPI-like anchor contains a phosphoceramide lipid group By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 72 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Transferase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 519	494	1,3-beta-glucanosyltransferase gel4		PRO_0000245553
	Propeptide	520 – 548	29	Removed in mature form Potential		PRO_0000245554
Regions
	Region	133 – 141	9	Donor substrate binding By similarity
Sites
	Active site	175	1	Proton donor By similarity
	Active site	277	1	Nucleophile By similarity
	Binding site	106	1	Donor substrate; via carbonyl oxygen By similarity
	Binding site	174	1	Donor substrate By similarity
	Binding site	175	1	Acceptor substrate By similarity
	Binding site	217	1	Acceptor substrate; via carbonyl oxygen By similarity
	Binding site	222	1	Acceptor substrate By similarity
	Binding site	309	1	Donor substrate By similarity
Amino acid modifications
	Lipidation	519	1	GPI-like-anchor amidated alanine Potential
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	181	1	N-linked (GlcNAc...) Potential
	Glycosylation	425	1	N-linked (GlcNAc...) Potential
	Disulfide bond	88 ↔ 117		By similarity
	Disulfide bond	249 ↔ 280		By similarity
	Disulfide bond	386 ↔ 437		By similarity
	Disulfide bond	395 ↔ 461		By similarity
	Disulfide bond	414 ↔ 419		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C956 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 191DA9A1D29778FE	FASTA	548	58,873
	10 20 30 40 50 60 MKFVYAAAGA SLVGSALATL PVIEAKVLKA CCWTIALDAN SYGNKFFYSN NGTEFFIRGV 70 80 90 100 110 120 AYQQEYQANG TSTENSDYTD PLANVDNCKR DIPYLKQLRT NVIRTYAVDP TKDHDECMKL 130 140 150 160 170 180 LDDAGIYLIT DLSAPSESIN RADPAWNTDL YKRYTSVIDA FAKYSNVIGF FAGNEVANDN 190 200 210 220 230 240 NNTNSIAYVK AAVRDMKSYI KSKDYRSSLL VGYATDDDAH IRADLADYLV CGDKESSIDM 250 260 270 280 290 300 FGYNIYEWCG DSSFEKSGYK DRTEEFSKYP VPAFFSEYGC IDPKPRKFTD VAALYGPQMN 310 320 330 340 350 360 DVWSGGIVYM YFQEANDYGL VSVSGDNVKT KEDFSYLSVQ MQKVTATGVN SASYTASNTA 370 380 390 400 410 420 VPTCPSVGAK WEASNKLPPS PNSELCDCMV ETLSCTVKDS VDEKEYGDLF DYLCAAGVCG 430 440 450 460 470 480 GINSNSTSGD YGAYSVCSAK QKLSFVMNQY YKKNNKAATA CDFDGKAQTK KGADASGSCA 490 500 510 520 530 540 SLISQAGTAG TGSVTAGATG SSGSGSASET SKGAAGVAAS PMAVKVGNWQ FGAYIATALF AGVGMLVL

References

[1]

"Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus." Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L. , Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., Barrell B.G., Denning D.W. Nature 438:1151-1156(2005) [PubMed: 16372009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAHF01000008 Genomic DNA. Translation: EAL87626.1.
RefSeq	XP_749664.1. XM_744571.1.
3D structure databases
ProteinModelPortal	P0C956.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADAFUAT00003468; CADAFUAP00003468; CADAFUAG00003468.
GeneID	3506753.
GenomeReviews	Gene locus gel4 in contig CM000170_GR.
KEGG	afm:AFUA_2G05340.
Phylogenomic databases
eggNOG	fuNOG06278.
GeneTree	EFGT00050000000135.
HOGENOM	HBG737647.
OMA	SEYGCID.
OrthoDB	EOG4617C9.
Family and domain databases
InterPro	IPR004886. GAS1. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR012946. X8. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF03198. Glyco_hydro_72. 1 hit. PF07983. X8. 1 hit. [Graphical view]
SMART	SM00768. X8. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GEL4_ASPFU

Accession

Primary (citable) accession number: P0C956
Secondary accession number(s): Q4WHH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program