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Protein

1,4-beta-D-glucan glucohydrolase

Gene

bglA

Organism
Thermotoga neapolitana
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Broad substrate specificity glycosidase. Releases glucose from soluble glucooligomers, with a preference for longer oligomers; acts more readily on cellotetraose than on cellobiose. Displays similar activities towards the disaccharides lactose and cellobiose. Is also able to hydrolyze various aryl-beta-glycosides in vitro.By similarity

Catalytic activityi

Hydrolysis of (1->4)-linkages in (1->4)-beta-D-glucans, to remove successive glucose units.By similarity
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641Proton donorSequence Analysis
Active sitei349 – 3491NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  1. glucan 1,4-beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayiUPA00350.
UPA00696.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-beta-D-glucan glucohydrolaseBy similarity (EC:3.2.1.74By similarity)
Short name:
Glucan glucohydrolaseBy similarity
Alternative name(s):
Beta-D-glucoside glucohydrolase
Beta-glucosidase (EC:3.2.1.21By similarity)
Glucan 1,4-beta-glucosidase
Gene namesi
Name:bglA
Synonyms:gghA
OrganismiThermotoga neapolitana
Taxonomic identifieri2337 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4254251,4-beta-D-glucan glucohydrolasePRO_0000063880Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C946.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017736. Glyco_hydro_1_beta-glucosidase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR03356. BGL. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P0C946-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKFPEGFLW GVATASYQIE GSPLADGAGM SIWHTFSHTP GNVKNGDTGD
60 70 80 90 100
VACDHYNRWK EDIEIIEKIG AKAYRFSISW PRILPEGTGK VNQKGLDFYN
110 120 130 140 150
RIIDTLLEKN ITPFITIYHW DLPFSLQLKG GWANRDIADW FAEYSRVLFE
160 170 180 190 200
NFGDRVKHWI TLNEPWVVAI VGHLYGVHAP GMKDIYVAFH TVHNLLRAHA
210 220 230 240 250
KSVKVFRETV KDGKIGIVFN NGYFEPASER EEDIRAARFM HQFNNYPLFL
260 270 280 290 300
NPIYRGEYPD LVLEFAREYL PRNYEDDMEE IKQEIDFVGL NYYSGHMVKY
310 320 330 340 350
DPNSPARVSF VERNLPKTAM GWEIVPEGIY WILKGVKEEY NPQEVYITEN
360 370 380 390 400
GAAFDDVVSE GGKVHDQNRI DYLRAHIEQV WRAIQDGVPL KGYFVWSLLD
410 420
NFEWAEGYSK RFGIVYVDYN TQKRI
Length:425
Mass (Da):49,366
Last modified:May 5, 2009 - v1
Checksum:iE66E33B75266187B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei425 – 4251

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97212 Genomic DNA. Translation: CAB10165.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97212 Genomic DNA. Translation: CAB10165.1.

3D structure databases

ProteinModelPortaliP0C946.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00350.
UPA00696.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017736. Glyco_hydro_1_beta-glucosidase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR03356. BGL. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression in Escherichia coli of Thermotoga neapolitana genes coding for enzymes of carbohydrate substrate degradation."
    Dakhova O., Kurepina N., Zverlov V., Svetlichnyi V., Velikodvorskaya G.
    Biochem. Biophys. Res. Commun. 194:1359-1364(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Z2706-MC24.

Entry informationi

Entry nameiBGLA_THENE
AccessioniPrimary (citable) accession number: P0C946
Secondary accession number(s): O33843, O52505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: February 4, 2015
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.