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P0C918 (RUS2_THIFE) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 10. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Rusticyanin
Gene names
Name:rus
Synonyms:rusTA
OrganismThiobacillus ferrooxidans (Acidithiobacillus ferrooxidans)
Taxonomic identifier920 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Electron carrier from cytochrome c552 to the A-type oxidase.

Cofactor

Binds 1 copper ion per subunit.

Subunit structure

Monomer.

Subcellular location

Periplasm.

Induction

Expressed during growth on Fe2+, sulfur and thiosulfate. Ref.1

Sequence similarities

Contains 1 plastocyanin-like domain.

Caution

The two different versions of rusticyanins are most probably due to strain variations.

Biophysicochemical properties

Redox potential:

E0 is +680 mV.

Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandCopper
Metal-binding
   Technical term3D-structure
Gene Ontology (GO)
   Biological processelectron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncopper ion binding

Inferred from electronic annotation. Source: InterPro

electron carrier activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 By similarity
Chain33 – 187155Rusticyanin
PRO_0000002872

Regions

Domain85 – 187103Plastocyanin-like

Sites

Metal binding1171Copper
Metal binding1701Copper
Metal binding1751Copper
Metal binding1801Copper

Secondary structure

1 ................................ 187
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0C918 [UniParc].

Last modified April 14, 2009. Version 1.
Checksum: BA1DA44A5BE95981

FASTA18719,857
        10         20         30         40         50         60 
MYTQNTMKKN WYVTVGAAAA LAATVGMGTA MAGTLDSTWK EATLPQVKAM LEKDTGKVSG 

        70         80         90        100        110        120 
DTVTYSGKTV HVVAAAVLPG FPFPSFEVHD KKNPTLEIPA GATVDVTFIN TNKGFGHSFD 

       130        140        150        160        170        180 
ITKKGPPYAV MPVIDPIVAG TGFSPVPKDG KFGYTDFTWH PTAGTYYYVC QIPGHAATGM 


FGKIIVK

« Hide

References

[1]"Sequence and expression of the rusticyanin structural gene from Thiobacillus ferrooxidans ATCC33020 strain."
Bengrine A., Guiliani N., Appia-Ayme C., Jedlicki E., Holmes D.S., Chippaux M., Bonnefoy V.
Biochim. Biophys. Acta 1443:99-112(1998) [PubMed: 9838063] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
Strain: ATCC 33020 / 11Fe.
[2]"Multiple wavelength anomalous diffraction (MAD) crystal structure of rusticyanin: a highly oxidizing cupredoxin with extreme acid stability."
Walter R.L., Ealick S.E., Friedman A.M., Blake R.C. II, Proctor P., Shoham M.
J. Mol. Biol. 263:730-751(1996) [PubMed: 8947572] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[3]Zhao D., Shoham M.
Submitted (JAN-1998) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[4]"Structure determination of a 16.8 kDa copper protein at 2.1-A resolution using anomalous scattering data with direct methods."
Harvey I., Hao Q., Duke E.M., Ingledew W.J., Hasnain S.S.
Acta Crystallogr. D 54:629-635(1998) [PubMed: 9761859] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[5]"NMR solution structure of Cu(I) rusticyanin from Thiobacillus ferrooxidans: structural basis for the extreme acid stability and redox potential."
Botuyan M.V., Toy-Palmer A., Chung J., Blake R.C. II, Beroza P., Case D.A., Dyson H.J.
J. Mol. Biol. 263:752-767(1996) [PubMed: 8947573] [Abstract]
Cited for: STRUCTURE BY NMR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ006456 Genomic DNA. Translation: CAA07038.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1A3ZX-ray1.90A33-187[»]
1A8ZX-ray2.10A39-187[»]
1CURNMR-A33-187[»]
1E30X-ray1.50A/B33-187[»]
1GY1X-ray1.65A/B33-187[»]
1GY2X-ray1.82A/B33-187[»]
1RCYX-ray1.90A38-187[»]
2CAKX-ray1.27A35-187[»]
2CALX-ray1.10A35-187[»]
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16161.

Family and domain databases

InterProIPR000923. BlueCu_1.
IPR002355. Cu_oxidase_Cu_BS.
IPR008972. Cupredoxin.
IPR001243. Rusticyanin.
[Graphical view]
Gene3DG3DSA:2.60.40.420. Cupredoxin. 1 hit.
PfamPF00127. Copper-bind. 1 hit.
[Graphical view]
PRINTSPR00158. RUSTICYANIN.
SUPFAMSSF49503. Cupredoxin. 1 hit.
TIGRFAMsTIGR03095. Rusti_cyanin. 1 hit.
PROSITEPS00196. COPPER_BLUE. 1 hit.
PS00079. MULTICOPPER_OXIDASE1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUS2_THIFE
AccessionPrimary (citable) accession number: P0C918
Secondary accession number(s): P24930 expand/collapse secondary AC list , P74919, Q56297, Q56298
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 14, 2009
Last modified: September 21, 2011
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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