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Protein

Ubiquitin carboxyl-terminal hydrolase 22

Gene

USP22

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei174 – 1741NucleophilePROSITE-ProRule annotation
Active sitei468 – 4681Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri50 – 11061UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: GO_Central
  2. H4 histone acetyltransferase activity Source: Ensembl
  3. ligand-dependent nuclear receptor transcription coactivator activity Source: Ensembl
  4. ubiquitin-specific protease activity Source: GO_Central
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. histone deubiquitination Source: GO_Central
  3. histone ubiquitination Source: Ensembl
  4. positive regulation of mitotic cell cycle Source: Ensembl
  5. positive regulation of transcription, DNA-templated Source: Ensembl
  6. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  7. regulation of proteasomal protein catabolic process Source: GO_Central
  8. regulation of transcription, DNA-templated Source: GO_Central
  9. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.035.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 22 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 22
Ubiquitin thioesterase 22
Ubiquitin-specific-processing protease 22
Gene namesi
Name:USP22
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 19

Subcellular locationi

  1. Nucleus By similarity

GO - Cellular componenti

  1. SAGA complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Ubiquitin carboxyl-terminal hydrolase 22PRO_0000367510Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei118 – 1181N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Interactioni

Subunit structurei

Component of some SAGA transcription coactivator-HAT complexes, at least composed of ATXN7, ATXN7L3, ENY2, GCN5L2, SUPT3H, TAF10, TRRAP and USP22. Within the SAGA complex, ATXN7L3, ENY2 and USP22 form a subcomplex required for histone deubiquitination. Interacts directly with ATXN7L3; leading to its recritment to the SAGA complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011818.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini165 – 509345USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. UBP8 subfamily.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri50 – 11061UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00780000121889.
HOGENOMiHOG000007260.
HOVERGENiHBG058014.
InParanoidiP0C8Z3.
KOiK11366.
OMAiIHEHAKA.
OrthoDBiEOG7FR7G7.
TreeFamiTF323554.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C8Z3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDAELVVTPP GCAHLGSFKV DNWKQNLRAI YQCFVWSGSA EARKRKAKSC
60 70 80 90 100
VCHVCGLHLN RLHSCLHCVF FGCFTKKHIH EHAKSKRHNL AIELMYGGIY
110 120 130 140 150
CFLCQDYIYD KDIEIIAKEE QRKAWKMQGV GEKFSTWEPT KRELELLKHN
160 170 180 190 200
PKRRKITSNC TIGLRGLINL GNTCFMNCIV QALTHTPLLR DFFLSDRHRC
210 220 230 240 250
EMQSPSSCLV CEMSSLFQEF YSGHRSPHIP YKLLHLVWTH ARHLAGYEQQ
260 270 280 290 300
DAHEFLIAAL DVLHRHCKGD DNGKKANNPN HCNCIIDQIF TGGLQSDVTC
310 320 330 340 350
QVCHGVSTTI DPFWDISLDL PGSSTPFWPL SPGSESSVVN GESHVSGTTT
360 370 380 390 400
LTDCLRRFTR PEHLGSSAKI KCSGCHSYQE STKQLTMKKL PIVACFHLKR
410 420 430 440 450
FEHSAKLRRK ITTYVSFPLE LDMTPFMASS KESRMNGQYQ QPTDSLNNDN
460 470 480 490 500
KYSLFAVVNH QGTLESGHYT SFIRQHKDQW FKCDDAIITK ASIADVLDSE
510
GYLLFYHKQF LEYE
Length:514
Mass (Da):58,737
Last modified:March 24, 2009 - v1
Checksum:iD825824E36F6C7B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EE333442 mRNA. No translation available.
EH202007 mRNA. No translation available.
EV691513 mRNA. No translation available.
RefSeqiNP_001160039.1. NM_001166567.1.
UniGeneiBt.12486.

Genome annotation databases

EnsembliENSBTAT00000011818; ENSBTAP00000011818; ENSBTAG00000008978.
GeneIDi509694.
KEGGibta:509694.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EE333442 mRNA. No translation available.
EH202007 mRNA. No translation available.
EV691513 mRNA. No translation available.
RefSeqiNP_001160039.1. NM_001166567.1.
UniGeneiBt.12486.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011818.

Protein family/group databases

MEROPSiC19.035.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000011818; ENSBTAP00000011818; ENSBTAG00000008978.
GeneIDi509694.
KEGGibta:509694.

Organism-specific databases

CTDi23326.

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00780000121889.
HOGENOMiHOG000007260.
HOVERGENiHBG058014.
InParanoidiP0C8Z3.
KOiK11366.
OMAiIHEHAKA.
OrthoDBiEOG7FR7G7.
TreeFamiTF323554.

Miscellaneous databases

NextBioi20869082.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon, Brain cortex and Rumen.

Entry informationi

Entry nameiUBP22_BOVIN
AccessioniPrimary (citable) accession number: P0C8Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: April 1, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.