P0C8Z3 (UBP22_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 22
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 22
Ubiquitin thioesterase 22
Ubiquitin-specific-processing protease 22

Gene names

Name:

USP22

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Component of some SAGA transcription coactivator-HAT complexes, at least composed of ATXN7, ATXN7L3, ENY2, GCN5L2, SUPT3H, TAF10, TRRAP and USP22. Within the SAGA complex, ATXN7L3, ENY2 and USP22 form a subcomplex required for histone deubiquitination. Interacts directly with ATXN7L3; leading to its recritment to the SAGA complex By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the peptidase C19 family. UBP8 subfamily. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation Ubl conjugation pathway
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator Chromatin regulator Hydrolase Protease Thiol protease
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW histone deubiquitination Inferred from electronic annotation. Source: Compara histone ubiquitination Inferred from electronic annotation. Source: Compara positive regulation of mitotic cell cycle Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	SAGA complex Inferred from electronic annotation. Source: Compara
Molecular_function	H4 histone acetyltransferase activity Inferred from electronic annotation. Source: Compara ligand-dependent nuclear receptor transcription coactivator activity Inferred from electronic annotation. Source: Compara ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	Ubiquitin carboxyl-terminal hydrolase 22		PRO_0000367510
Regions
	Zinc finger	50 – 110	61	UBP-type
Sites
	Active site	174	1	Nucleophile By similarity
	Active site	468	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	118	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C8Z3 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: D825824E36F6C7B4	FASTA	514	58,737
	10 20 30 40 50 60 MDAELVVTPP GCAHLGSFKV DNWKQNLRAI YQCFVWSGSA EARKRKAKSC VCHVCGLHLN 70 80 90 100 110 120 RLHSCLHCVF FGCFTKKHIH EHAKSKRHNL AIELMYGGIY CFLCQDYIYD KDIEIIAKEE 130 140 150 160 170 180 QRKAWKMQGV GEKFSTWEPT KRELELLKHN PKRRKITSNC TIGLRGLINL GNTCFMNCIV 190 200 210 220 230 240 QALTHTPLLR DFFLSDRHRC EMQSPSSCLV CEMSSLFQEF YSGHRSPHIP YKLLHLVWTH 250 260 270 280 290 300 ARHLAGYEQQ DAHEFLIAAL DVLHRHCKGD DNGKKANNPN HCNCIIDQIF TGGLQSDVTC 310 320 330 340 350 360 QVCHGVSTTI DPFWDISLDL PGSSTPFWPL SPGSESSVVN GESHVSGTTT LTDCLRRFTR 370 380 390 400 410 420 PEHLGSSAKI KCSGCHSYQE STKQLTMKKL PIVACFHLKR FEHSAKLRRK ITTYVSFPLE 430 440 450 460 470 480 LDMTPFMASS KESRMNGQYQ QPTDSLNNDN KYSLFAVVNH QGTLESGHYT SFIRQHKDQW 490 500 510 FKCDDAIITK ASIADVLDSE GYLLFYHKQF LEYE

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Ascending colon, Brain cortex and Rumen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EE333442 mRNA. No translation available. EH202007 mRNA. No translation available. EV691513 mRNA. No translation available.
IPI	IPI00707759.
RefSeq	NP_001160039.1. NM_001166567.1.
UniGene	Bt.12486.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000011818.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000011818; ENSBTAP00000011818; ENSBTAG00000008978.
GeneID	509694.
KEGG	bta:509694.
Organism-specific databases
CTD	23326.
Phylogenomic databases
eggNOG	COG5533.
GeneTree	ENSGT00690000101718.
HOGENOM	HOG000007260.
HOVERGEN	HBG058014.
KO	K11366.
OMA	DMEMIAK.
OrthoDB	EOG48PMJS.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20869082.

Entry information

Entry name

UBP22_BOVIN

Accession

Primary (citable) accession number: P0C8Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	April 3, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program