P0C8Y9 (PSA4B_ORYSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome subunit alpha type-4-2
EC=3.4.25.1

Alternative name(s):
20S proteasome alpha subunit C
20S proteasome subunit alpha-3

Gene names

ORF Names:

OsI_021067, OsI_21826

Organism

Oryza sativa subsp. indica (Rice)

Taxonomic identifier

39946 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Proteasome subunit alpha type-4-2		PRO_0000361769

Sequences

	Sequence		Length	Mass (Da)
	P0C8Y9 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: F5FDAD81AB27AA65	FASTA	250	27,026
	10 20 30 40 50 60 MSRRYDSRTT IFSPEGRLYQ VEYAMEAIGN AGSALGVLAA DGVVLVGEKK VTSKLLQTSR 70 80 90 100 110 120 SAEKMYKIDS HLACAVAGIM SDANILLNTA RLHAQRYALS YQEPIPVEQL VQSLCDTKQG 130 140 150 160 170 180 YTQFGGLRPF GVSFLFAGWD KHHGFQLYMS DPSGNYSGWK AAAVGANSQA AQSMLKQDYR 190 200 210 220 230 240 DGLTREEAVA LALKVLSKTM DSTSLTAEKL ELAEVFLQPG TGEVQYQVCS PEAMGKLLAK 250 AGLSQPAPEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. 93-11.
[2]	"Interactive genomics and proteomics of plant growth promoting rhizobacteria (PGPR) for the management of major pests and diseases in rice." Saveetha K., Samiyappan R., Raveendran M., Balasubramanian P. Submitted (NOV-2008) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: cv. CO43. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000131 Genomic DNA. Translation: EEC80089.1.
3D structure databases
ProteinModelPortal	P0C8Y9.
SMR	P0C8Y9. Positions 2-220.
ModBase	Search...
Protein-protein interaction databases
STRING	P0C8Y9.
Proteomic databases
PRIDE	P0C8Y9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os06g07140.1; LOC_Os06g07140.1; LOC_Os06g07140.
Phylogenomic databases
GeneTree	EPGT00050000005277.
PhylomeDB	P0C8Y9.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_N. IPR023332. Proteasome_suA-type. IPR001353. Proteasome_sua/b. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
SMART	SM00948. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA4B_ORYSI

Accession

Primary (citable) accession number: P0C8Y9
Secondary accession number(s): B8B392

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program