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P0C8W4 (KBX2_TITST) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel toxin TstKMK
Alternative name(s):
Toxin 5536
beta-Ktx
OrganismTityus stigmurus (Brazilian scorpion)
Taxonomic identifier50344 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeTityus

Protein attributes

Sequence length91 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Blocks voltage-gated potassium channels (Kv) By similarity.

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Sequence similarities

Belongs to the long chain scorpion toxin family. Class 2 subfamily.

Caution

A mature protein with 47 amino acid residues is predicted in Ref.2 (sequence of 45-91) instead of the chain proposed here (2 amino acids longer). Since the protein sequence and the predicted mass correspond exactly with proteomic data (Ref.2), the 49 amino acid chain is shown.

Mass spectrometry

Molecular mass is 5536.0 Da from positions 43 - 91. Determined by ESI. Ref.2

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Propeptide26 – 4217
PRO_0000421251
Chain43 – 9149Potassium channel toxin TstKMK
PRO_0000366102

Amino acid modifications

Disulfide bond61 ↔ 81 By similarity
Disulfide bond68 ↔ 86 By similarity
Disulfide bond72 ↔ 88 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0C8W4 [UniParc].

Last modified February 6, 2013. Version 2.
Checksum: 39EED225DDFC4589

FASTA9110,097
        10         20         30         40         50         60 
MVATNRCCVF ALLFALLLVH SLTEAGKGKE ILGKIKEKII EAKDKMKAGW ERLTSQSEYA 

        70         80         90 
CPAIDKFCED HCAAKKAVGK CDDFKCKCIK L 

« Hide

References

[1]"Molecular approaches for structural characterization of a new potassium channel blocker from Tityus stigmurus venom: cDNA cloning, homology modeling, dynamic simulations and docking."
Almeida D.D., Torres T.M., Barbosa E.G., Lima J.P., de Freitas Fernandes-Pedrosa M.
Biochem. Biophys. Res. Commun. 430:113-118(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], 3D-STRUCTURE MODELING.
Tissue: Venom gland.
[2]"Proteomic analysis of the venom from the scorpion Tityus stigmurus: biochemical and physiological comparison with other Tityus species."
Batista C.V.F., Roman-Gonzalez S.A., Salas-Castillo S.P., Zamudio F.Z., Gomez-Lagunas F., Possani L.D.
Comp. Biochem. Physiol. 146C:147-157(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 43-57, MASS SPECTROMETRY.
Tissue: Venom.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
JK483711 mRNA. No translation available.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameKBX2_TITST
AccessionPrimary (citable) accession number: P0C8W4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: February 6, 2013
Last modified: October 16, 2013
This is version 12 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Scorpion potassium channel toxins

Nomenclature of scorpion potassium channel toxins and list of entries