Kunitz-type serine protease inhibitor Hg1 precursor

Functionⁱ

Dual-function toxin that completely inhibits the activity of trypsin at a molar ratio of 1:1 (dissociation constant of 107 nM) and that inhibits mKv1.3/KCNA3 potassium channel currents with an IC₅₀ of 6.2 nM (where 1 µM inhibits 80% of currents). Also has weak inhibitory activity against mKv1.1/KCNA1 (where 1 µM inhibits less than 50% of currents), hKv1.2/KCNA2 (where 1 µM inhibits less than 50% of currents), and hKCa2.3/KCNN3.1 Publication

Miscellaneous

Has no effect on chymotrypsin, nor elastase, nor the KCa1.1/KCNMA1 potassium channel.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	39 – 40	Reactive bond for trypsinBy similarity		2
Siteⁱ	77	Pore-blocking residueCurated		1

GO - Molecular functionⁱ

serine-type endopeptidase inhibitor activity
Source: UniProtKB
Inferred from direct assayⁱ
- 22354971

Complete GO annotation...

GO - Biological processⁱ

envenomation resulting in negative regulation of voltage-gated potassium channel activity in other organism
Source: UniProtKB
Inferred from direct assayⁱ
- 22354971

Complete GO annotation...

Keywordsⁱ

Molecular function	Ion channel impairing toxin, Potassium channel impairing toxin, Protease inhibitor, Serine protease inhibitor, Toxin, Voltage-gated potassium channel impairing toxin

Molecular function

Ion channel impairing toxin, Potassium channel impairing toxin, Protease inhibitor, Serine protease inhibitor, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Kunitz-type serine protease inhibitor Hg1 Alternative name(s): Delta-KTx 1.1
Organismⁱ	Hadrurus gertschi (Scorpion)
Taxonomic identifierⁱ	380989 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Iurida › Iuroidea › Caraboctonidae › Hadrurinae › Hadrurus

Subcellular locationⁱ

Secreted By similarity

GO - Cellular componentⁱ

extracellular region
Source: UniProtKB
Non-traceable author statementⁱ
- 22354971
extracellular space of host
Source: UniProtKB
Non-traceable author statementⁱ
- 22354971

Complete GO annotation...

Keywords - Cellular componentⁱ

Secreted

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	77	K → A: 94-fold reduction in KCNA3 inhibition. 1 Publication	1
Mutagenesisⁱ	78	R → A: 49-fold reduction in KCNA3 inhibition. 1 Publication	1
Mutagenesisⁱ	82	F → A: 58-fold reduction in KCNA3 inhibition. 1 Publication	1
Mutagenesisⁱ	84	K → A: 74-fold reduction in KCNA3 inhibition. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 21	Sequence analysisAdd BLAST		21
ChainⁱPRO_0000366101	22 – 88	Kunitz-type serine protease inhibitor Hg1Add BLAST		67

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	29 ↔ 79	PROSITE-ProRule annotation
Disulfide bondⁱ	38 ↔ 62	PROSITE-ProRule annotation
Disulfide bondⁱ	54 ↔ 75	PROSITE-ProRule annotation
Glycosylationⁱ	68	N-linked (GlcNAc...) asparagineSequence analysis	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Expressionⁱ

Tissue specificityⁱ

Expressed by the venom gland.

Structureⁱ

3D structure databases

SMRⁱ	P0C8W3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	29 – 79	BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd BLAST		51

Sequence similaritiesⁱ

Belongs to the venom Kunitz-type family. Scorpion delta-Ktx subfamily. Delta-Ktx 1 sub-subfamily.Curated

Keywords - Domainⁱ

Signal

Family and domain databases

Gene3Dⁱ	4.10.410.10. 1 hit.
InterProⁱ	View protein in InterPro IPR002223. Kunitz_BPTI. IPR020901. Prtase_inh_Kunz-CS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00014. Kunitz_BPTI. 1 hit.
PRINTSⁱ	PR00759. BASICPTASE.
SMARTⁱ	View protein in SMART SM00131. KU. 1 hit.
SUPFAMⁱ	SSF57362. SSF57362. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00280. BPTI_KUNITZ_1. 1 hit. PS50279. BPTI_KUNITZ_2. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P0C8W3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MIIFYGLFSI LVLTSINIAE AGHHNRVNCL LPPKTGPCKG SFARYYFDIE 
        60         70         80 
TGSCKAFIYG GCEGNSNNFS EKHHCEKRCR GFRKFGGK

Length:88

Mass (Da):9,855

Last modified:March 3, 2009 - v1

Checksum:ⁱ03698FA348984FE9

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	EL698904 mRNA. No translation available.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

EL698904 mRNA. No translation available.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	EL698904 mRNA. No translation available.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

EL698904 mRNA. No translation available.

3D structure databases

SMRⁱ	P0C8W3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Family and domain databases

Gene3Dⁱ	4.10.410.10. 1 hit.
InterProⁱ	View protein in InterPro IPR002223. Kunitz_BPTI. IPR020901. Prtase_inh_Kunz-CS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00014. Kunitz_BPTI. 1 hit.
PRINTSⁱ	PR00759. BASICPTASE.
SMARTⁱ	View protein in SMART SM00131. KU. 1 hit.
SUPFAMⁱ	SSF57362. SSF57362. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00280. BPTI_KUNITZ_1. 1 hit. PS50279. BPTI_KUNITZ_2. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	VKT11_HADGE
Accessionⁱ	P0C8W3Primary (citable) accession number: P0C8W3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
	Last sequence update:	March 3, 2009
	Last modified:	May 10, 2017
	This is version 26 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Animal Toxin Annotation Program

Miscellaneousⁱ

Documents

Scorpion potassium channel toxins
Nomenclature of scorpion potassium channel toxins and list of entries
SIMILARITY comments
Index of protein domains and families

UniProtKB - P0C8W3 (VKT11_HADGE)

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Kunitz-type serine protease inhibitor Hg1

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Functionⁱ

Miscellaneous

Sites

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Mutagenesis

PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Expressionⁱ

Tissue specificityⁱ

Structureⁱ

3D structure databases

Family & Domainsⁱ

Domains and Repeats

Sequence similaritiesⁱ

Keywords - Domainⁱ

Family and domain databases

Sequenceⁱ

Sequence databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Documents

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0C8W3 (VKT11_HADGE)

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Kunitz-type serine protease inhibitor Hg1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ