P0C8S4 (RPOB_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	CBU_0231

Organism

Coxiella burnetii

Taxonomic identifier

777 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella

Protein attributes

Sequence length	1375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.
Sequence caution	The sequence AAO89789.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1375	1375	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000047890

Sequences

	Sequence		Length	Mass (Da)
	P0C8S4 [UniParc]. Last modified March 3, 2009. Version 1. Checksum: D3E6EEAA861F6403	FASTA	1,375	153,656
	10 20 30 40 50 60 MAQANSRSRN SATISYPEKR RARVNLGKRE VEILKTPYLL ETQIESYRKF LQKDIAAEKR 70 80 90 100 110 120 EDNGLHAAFK SVFPITSYSG YAVLEYVGYS LGGESTLFDV EECKLRGLTY AAPLKVNMRL 130 140 150 160 170 180 VIYDKEAPAG KKAIKDIKEQ EVYMGEIPLM TETGSLVING TERVVVSQLH RSPGVFFEHD 190 200 210 220 230 240 KGKTHSSGKL LYSARVIPYR GSWLDFEFDP KDCLFVRIDR RRKLPATVIL RALGYDTEQI 250 260 270 280 290 300 LDMFYKTNHF HLNQETVTLD LIPQRLRGEL AVVEIKDKKG KVIVEANRRI SARHIRLIEK 310 320 330 340 350 360 AEINKLELPD DYLYGKVIGK TIIDKETGEI IAHANEEITA ELLKNLRATK TASLDTLYIN 370 380 390 400 410 420 EIECGPYVSD TLRLDTTTNQ LEALVEIYRI MRPGEPPTKE AAESLFENLF FSPERYSLSA 430 440 450 460 470 480 VGRMKFNRRV GRKELTGLDV LSKEDIVDVL RVLVDIRDGK GDVDDIDHLG NRRIRSVGEM 490 500 510 520 530 540 AENQFRVGLV RVERAVKDRL SLADVENLMP QDLVNAKPVS AAIKEFFGSS QLSQFMDQNN 550 560 570 580 590 600 PLSEITHKRR VSALGPGGLT RERAGFEVRD VHVTHYGRVC PIETPEGPNI GLINSLAVFA 610 620 630 640 650 660 RANEYGFLET PYRKVVDRVV TDETEYLSAI EEGDYYIAQA NTNVDEKGRL VDDLISCRYK 670 680 690 700 710 720 GEFTLTTPDK INYMDVSPRQ IVSVAAALIP FLEHDDANRA LMGSNMQRQA VPTIRPETPL 730 740 750 760 770 780 VGTGMERTVA VDSGVTVIAK RSGVIDSVDA SRIVVRVDRK ETEDDDDIGV DIYNLTKFTR 790 800 810 820 830 840 SNQNTCINQH PIVEVGDKVQ KGDVLADGPS TDIGELALGQ NLLVAFMPWN GYNFEDSILI 850 860 870 880 890 900 SERLVEEDRF TTIHIQEFTC VARDTKLGPE EITSDIPNVG ESALAKLDES GIVHIGAEVN 910 920 930 940 950 960 AGDILVGKVT PKGETQLTPE EKLLRAIFGE KASDVKDTSL RVTPGITGTV IDVRIFTREG 970 980 990 1000 1010 1020 IKKDERTLEI EKAELSKVEK DLNDELRVRE DALFENLEKL LTGRVAAGGP NKLAKGTKIT 1030 1040 1050 1060 1070 1080 KSYLADLPRQ KWFEIRLQDD AATKRLEASH EHFKELRETR DAKLKDSRQK LTQGGDLAPG 1090 1100 1110 1120 1130 1140 VIKIVKVYLA VKRRIQPGDK MAGRHGNKGV ISTIVPIEDM PYLEDGTPVD IVLNPLGVPS 1150 1160 1170 1180 1190 1200 RMNIGQVLET HLGWAAKGLG KKIGEMIEKG ADAKELRKSL KPIYDLSKTQ RFDLEALEDP 1210 1220 1230 1240 1250 1260 EIVTLAKNLR KGVPISSPVF DGATEEEIKQ LLKMADLPTS GQAALYDGRT GKKFDRSVTV 1270 1280 1290 1300 1310 1320 GYMYMLKLNH LVDDKMHARS TGSYSLVTQQ PLGGKAQFGG QRFGEMEVWA LEAYGAAYTL 1330 1340 1350 1360 1370 QEMLTVKSDD VAGRTRMYKN IVDGDHRMDA GMPESFNVLV KEIRSLAIDI GLEND

References

[1]

"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed: 12704232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nine Mile phase I / RSA 493.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016828 Genomic DNA. Translation: AAO89789.2. Different initiation.
RefSeq	NP_819275.2. NC_002971.3.
3D structure databases
ProteinModelPortal	P0C8S4.
ModBase	Search...
Proteomic databases
PRIDE	P0C8S4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1208112.
GenomeReviews	Gene locus CBU_0231 in contig AE016828_GR.
KEGG	cbu:CBU_0231.
PATRIC	17929151. VBICoxBur82552_0224.
TIGR	CBU_0231.
Phylogenomic databases
HOGENOM	HBG285547.
OMA	HANEEIT.
PhylomeDB	P0C8S4.
ProtClustDB	PRK00405.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_COXBU

Accession

Primary (citable) accession number: P0C8S4
Secondary accession number(s): O87903

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program