Reviewed, UniProtKB/Swiss-Prot P0C8R1 (KAX3C_ANDAM)

Names and origin

Protein names	Recommended name:     Potassium channel toxin alpha-KTx 3.12 Alternative name(s):     Kaliotoxin analog       Short name=Aam-KTX
Organism	Androctonus amoreuxi (African fattail scorpion)
Taxonomic identifier	112024 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Androctonus

Protein attributes

Sequence length	38 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Potent inhibitor of voltage-dependent potassium channels, with a preference for Kv1.3 (KCNA3) versus Kv1.2 (KCNA2). Has no effect on Kv1.1 (KCNA1). Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Toxic dose	LD50 is 5 µg/kg by intracerebroventricular injection into mice (100ng/mouse). Ref.1
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 3 subfamily.
Mass spectrometry	Molecular mass is 4184.08 Da from positions 1 - 38. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ionic channel inhibitor Neurotoxin Potassium channel inhibitor Toxin
PTM	Amidation Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 38	38	Potassium channel toxin alpha-KTx 3.12		PRO_0000363663
Amino acid modifications
	Modified residue	38	1	Lysine amide By similarity
	Disulfide bond	8 ↔ 28		By similarity
	Disulfide bond	14 ↔ 33		By similarity
	Disulfide bond	18 ↔ 35		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C8R1-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 11C0088FD6A021D4	FASTA	38	4,192
	10 20 30 GVEINVKCTG SHQCIKPCKD AGMRFGKCIN RKCHCTPK

References

[1]

"A new kaliotoxin selective towards Kv1.3 and Kv1.2 but not Kv1.1 channels expressed in oocytes." Abbas N., Belghazi M., Abdel-Mottaleb Y., Tytgat J., Bougis P.E., Martin-Eauclaire M.-F. Biochem. Biophys. Res. Commun. 376:525-530(2008) [PubMed: 18804453] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, LETHAL DOSE. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001947. Scorpion_toxinS. [Graphical view]
Pfam	PF00451. Toxin_2. 1 hit. [Graphical view]
PRINTS	PR00286. CHARYBDTOXIN.
ProDom	PD003586. Scorpion_toxinS. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01138. SCORP_SHORT_TOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAX3C_ANDAM

Accession

Primary (citable) accession number: P0C8R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	November 24, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)