Reviewed, UniProtKB/Swiss-Prot P0C8P4 (KYNB_RALME)

Names and origin

Protein names

Recommended name:
    Kynurenine formamidase
      Short name=KFA
    EC=3.5.1.9
Alternative name(s):
    N-formylkynurenine formamidase

Gene names

Name:	kynB
Ordered Locus Names:	Rmet_2648

Organism

Ralstonia metallidurans (strain CH34 / ATCC 43123 / DSM 2839) [Complete proteome] [HAMAP]

Taxonomic identifier

266264 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine. Ref.2 Ref.3
Catalytic activity	N-formyl-L-kynurenine + H2O = formate + L-kynurenine. Ref.2 Ref.3
Enzyme regulation	Inhibited by EDTA. Insensitive to PMSF. Ref.2 Ref.3
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2. Ref.3
Sequence similarities	Belongs to the kynB family.
Biophysicochemical properties	Kinetic parameters: KM=75 µM for N-formyl-L-kynurenine pH dependence: Optimum pH is 8.0.
Sequence caution	The sequence CP000352 differs from that shown. Reason: Erroneous termination at position 83. Translated as Tyr.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anthranilate metabolic process Ref.3 Inferred from direct assay. Source: UniProtKB tryptophan catabolic process to kynurenine Ref.3 Inferred from direct assay. Source: UniProtKB
Molecular function	arylformamidase activity Ref.3 Inferred from direct assay. Source: UniProtKB metal ion binding Ref.3 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Kynurenine formamidase		PRO_0000362135

Sequences

	Sequence		Length	Mass (Da)
	P0C8P4-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 43F8AEFAEA1959D9	FASTA	218	23,383
	10 20 30 40 50 60 MPQAPQLHDG RRIWDISPAV SPATPVWPGD TPFQHDPAWQ LDEHCPVNVG RITMSPHTGA 70 80 90 100 110 120 HADAPLHYAA DGAPIGAVPL DAYLGPCRVI HCIGAAPRVE PQHIAHALAG TPPRVLLRTY 130 140 150 160 170 180 AQAPQGKWDS AFCAVAPETI SLLARHGVRL IGIDTPSLDP ETSKTMDAHH AVRDHQLAIL 190 200 210 EGIVLDEVPA GDYELIALPL RLATLDASPV RAVLRELP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of the chromosome of Ralstonia metallidurans CH34." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Martinez M., Goltsman E., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Mergeay M., Benotmane M.A., Vallaeys T., Michaux A., Monchy S., Dunn J., McCorkle S., Taghavi S., van der Lelie D., Richardson P. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"NAD biosynthesis: identification of the tryptophan to quinolinate pathway in bacteria." Kurnasov O., Goral V., Colabroy K., Gerdes S., Anantha S., Osterman A., Begley T.P. Chem. Biol. 10:1195-1204(2003) [PubMed: 14700627] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
[3]	"Aerobic tryptophan degradation pathway in bacteria: novel kynurenine formamidase." Kurnasov O., Jablonski L., Polanuyer B., Dorrestein P., Begley T., Osterman A. FEMS Microbiol. Lett. 227:219-227(2003) [PubMed: 14592712] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, PATHWAY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000352 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus Rmet_2648 in contig CP000352_GR.
KEGG	rme:Rmet_2648.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR017484. Arylformamidase. IPR007325. Cyclase. [Graphical view]
Pfam	PF04199. Cyclase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03035. trp_arylform. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KYNB_RALME

Accession

Primary (citable) accession number: P0C8P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	May 26, 2009
This is version 5 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)