Reviewed, UniProtKB/Swiss-Prot P0C8N8 (UCRI_THEEB)

Names and origin

Protein names

Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit
    EC=1.10.99.1
Alternative name(s):
    Rieske iron-sulfur protein
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
      Short name=ISP
      Short name=RISP

Gene names

Name:	petC
Ordered Locus Names:	tlr0959

Organism

Thermosynechococcus elongatus (strain BP-1) [Complete proteome] [HAMAP]

Taxonomic identifier

197221 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Thermosynechococcus

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity. HAMAP MF_01335
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin. HAMAP MF_01335
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. HAMAP MF_01335
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity. HAMAP MF_01335
Subcellular location	Cellular thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity. HAMAP MF_01335
Miscellaneous	The Rieske iron-sulfur protein is a high potential 2Fe-2S protein. HAMAP MF_01335
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Thylakoid
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: HAMAP ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	Cytochrome b6-f complex iron-sulfur subunit HAMAP MF_01335		PRO_0000361773
Regions
	Transmembrane	21 – 43	23	Potential
	Domain	66 – 162	97	Rieske
Sites
	Metal binding	108	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	110	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	126	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	129	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	113 ↔ 128		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C8N8-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: EBB4352DB5DAB6B7	FASTA	180	19,316
	10 20 30 40 50 60 MAQVSGMSDV PDMGRRQFMN LLTFGTITGT ALGALYPVVK YFIPPASGGT GGGAVAKDAL 70 80 90 100 110 120 GNDIKVSEYL AKHLPGDRSL AQGIKGDPTY VIVTEDHQIA NYGLNAVCTH LGCVVPWNVS 130 140 150 160 170 180 ENKFICPCHG SQYDSTGKVV RGPAPLSLAL VKATVTEDDK LVFTPWTEID FRTGKEPWWT

References

[1]

"Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1." Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S., Watanabe A., Iriguchi M., Kawashima K., Kimura T., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Nakazaki N., Shimpo S., Sugimoto M. , Takeuchi C., Yamada M., Tabata S. DNA Res. 9:123-130(2002) [PubMed: 12240834] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000039 Genomic DNA. Translation: BAC08511.1.
RefSeq	NP_681749.1.
3D structure databases
SMR	P0C8N8. Positions 9-179.
ModBase	Search...
Protein-protein interaction databases
STRING	P0C8N8.
Genome annotation databases
GeneID	1011363.
GenomeReviews	Gene locus tlr0959 in contig BA000039_GR.
KEGG	tel:tlr0959.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0723.
HOGENOM	HBG668715.
OMA	YPVANYF.
Family and domain databases
HAMAP	MF_01335. Cytb6_f_Rieske. [Tree]
InterPro	IPR014909. Cyt_b6-f_cplx_Fe-S_su. IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_THEEB

Accession

Primary (citable) accession number: P0C8N8
Secondary accession number(s): Q79V50, Q9X9T2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	January 19, 2010
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)