P0C8L1 (KA124_TITST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin alpha-KTx 12.4 Alternative name(s): Butantoxin Short name=BuTX Short name=TstBut
Organism	Tityus stigmurus (Brazilian scorpion)
Taxonomic identifier	50344 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Tityus

Protein attributes

Sequence length	40 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits high conductance calcium-activated potassium channels. Reversibly inhibits Shaker B potassium channels By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 12 subfamily.
Mass spectrometry	Molecular mass is 4506.6 Da from positions 1 - 40. Determined by ESI. Ref.2

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 40	40	Potassium channel toxin alpha-KTx 12.4		PRO_0000356889
Sites
	Site	30	1	Basic residue of the functional dyad By similarity
	Site	39	1	Aromatic residue of the functional dyad By similarity
Amino acid modifications
	Disulfide bond	2 ↔ 5		Ref.1
	Disulfide bond	10 ↔ 31		Ref.1
	Disulfide bond	16 ↔ 36		Ref.1
	Disulfide bond	20 ↔ 38		Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0C8L1 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 79C47786312FC1BD	FASTA	40	4,514
	10 20 30 40 WCSTCLDLAC GASRECYDPC FKAFGRAHGK CMNNKCRCYT

References

[1]	"NMR solution structure of butantoxin." Holaday S.K. Jr., Martin B.M., Fletcher P.L. Jr., Krishna N.R. Arch. Biochem. Biophys. 379:18-27(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, STRUCTURE BY NMR, DISULFIDE BONDS. Tissue: Venom.
[2]	"Proteomic analysis of the venom from the scorpion Tityus stigmurus: biochemical and physiological comparison with other Tityus species." Batista C.V.F., Roman-Gonzalez S.A., Salas-Castillo S.P., Zamudio F.Z., Gomez-Lagunas F., Possani L.D. Comp. Biochem. Physiol. 146C:147-157(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Venom.

Cross-references

3D structure databases
ProteinModelPortal	P0C8L1.
SMR	P0C8L1. Positions 1-40.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.30.10. 1 hit.
InterPro	IPR003614. Scorpion_toxin-like. IPR001947. Scorpion_toxinS_K_inh. [Graphical view]
Pfam	PF00451. Toxin_2. 1 hit. [Graphical view]
PRINTS	PR00286. CHARYBDTOXIN.
ProDom	PD003586. Scorpion_toxinS. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF57095. SSF57095. 1 hit.
PROSITE	PS01138. SCORP_SHORT_TOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KA124_TITST

Accession

Primary (citable) accession number: P0C8L1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	December 16, 2008
Last modified:	May 1, 2013
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program