P0C8K5 (RPB2_ASFWA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit 2
EC=2.7.7.6

Gene names

Ordered Locus Names:

War-063

Organism

African swine fever virus (isolate Warthog/Namibia/Wart80/1980) (ASFV) [Complete proteome]

Taxonomic identifier

561444 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]

Protein attributes

Sequence length	1242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the DNA-dependent RNA polymerase that catalyzes the transcription in the cytoplasm of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Subunit 2 is the second largest component of RNA polymerase. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. DNA-dependent RNA polymerase is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase that consists of at least 6 subunits Probable.
Subcellular location	Host cytoplasm Potential.
Miscellaneous	The binding of ribonucleoside triphosphate to the RNA polymerase transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol subunits By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Host cytoplasm
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1242	1242	DNA-directed RNA polymerase subunit 2		PRO_0000355628
Regions
	Zinc finger	1180 – 1201	22	C4-type

Sequences

	Sequence		Length	Mass (Da)
	P0C8K5 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: 10E23F427C1CA48E	FASTA	1,242	139,864
	10 20 30 40 50 60 MEPLRPQITY GPIETVDNEE LTEADMLSFI SAAVNSTGLI GYNIKSFDDL MDNGIPQIVK 70 80 90 100 110 120 QMFNVDITYK DQRDHTEIDK LRESVQIQFN FTDVNIERPQ HRNYSQGNKI NLLPNKARLC 130 140 150 160 170 180 GLSYSGPVNL AAEVILTAHY SNGRQEVKRA SIPPFQVSTF PIMRGSNRCH THHLSKTAKK 190 200 210 220 230 240 EIGEDPNEPG GYFIARGGEW VVDLLENIRF NTLHIHYHTM QQGNNEIIRG EFISQPGGAF 250 260 270 280 290 300 ENSSQIIIRY MTTGAITIEI NSTKFSKLRI PWYLIFRMFG MTGDDSIIEQ VVFDLESNSL 310 320 330 340 350 360 VNTFMIEILE KSIHVLDPIF QPVQHELNRE KIIQFLSEKV SKFVSNPSAY KSDENAVQYL 370 380 390 400 410 420 NERQLTILDK ILLPHMGQTA DTRVRKLRFL GLLIHKILLV IMNVFPPTDR DSYRTKRVHG 430 440 450 460 470 480 SGVSLAKAFK AIFNTSVIAP IINGFKELLK QTAFEELTQR NIIEAFSAAL SKNTASDLNR 490 500 510 520 530 540 SMEQSIISGN KTIMVRQRPI VNRVSTQSLE RKNLLNTISA LRTVNTHNTT NASKQTERAD 550 560 570 580 590 600 MMRRVHASYP GYICVAQSAD TGEKVGMSKQ LAITANVCTA GEVLSLKQRL LSDPAIQQLA 610 620 630 640 650 660 DVSNKDIVRK GLARVFINGE WIGCCTNAFE LAQRYRMLRR EGKIVHPHTT IYWDSMVDEV 670 680 690 700 710 720 EFWLDVGRLT RPLLIVDNNI EKYNQACYKA AEARKKGDKD WEKHKIPFVQ NTRFTSQMAK 730 740 750 760 770 780 DILAGTLTLE DLVAQGICEF ITPEEAENCL VAFSIIELRK HKHDVTRRFT HVDVPQAILG 790 800 810 820 830 840 LAALVSPYAN CTQPARVTYE TNQGRQTGGW YCFSWPYRVD MNRFFQFYNE MPLVKTIAHN 850 860 870 880 890 900 YVIPNGLNTI VAYMIYGGYN QEDSVIVSQS FIDRGGFAGT FYREEKVELE SDIESFGKPD 910 920 930 940 950 960 PLITKNLKPG ANYEKLVDGF VPVGTVVKKG DIIIGKVAKI RGEKDELNKY IDRSVMYGFD 970 980 990 1000 1010 1020 EPAVVDAVMR PHGPNDEIFG LMRLRYERNL NIGDKMSSRS GNKGIAALAL PTSDMPFTED 1030 1040 1050 1060 1070 1080 GLQPDLIVNP HSHPSRMTNG QMIETTVGLA NALQGVVTDG TAFLPINVQL LSERLAQEGL 1090 1100 1110 1120 1130 1140 RFNGCQKMFN GQTGEYFDAA IFIGPTYHQR LQKFVLDDRY AVASYGPTDA LTGQPLDGKR 1150 1160 1170 1180 1190 1200 SHGGLRLGEM EHWVLTAQGA MQTIIEKSHD DSDGCISYVC RNCGEPAIYN ASHPIYKCMN 1210 1220 1230 1240 CDVQADIGMV DSRRSSIVFQ HEMRAANVNI TSVLSPRVFQ PA

References

[1]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY261366 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007646. RNA_pol_Rpb2_4. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 1 hit.
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04566. RNA_pol_Rpb2_4. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPB2_ASFWA

Accession

Primary (citable) accession number: P0C8K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	December 16, 2008
Last modified:	March 8, 2011
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program