P0C8K0 (KBAZ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-tagatose-1,6-bisphosphate aldolase subunit KbaZ

Gene names

Name:	kbaZ
Synonyms:	agaZ, yhaX
Ordered Locus Names:	b3132, JW3101

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the tagatose-1,6-bisphosphate aldolase KbaYZ that is required for full activity and stability of the Y subunit. Could have a chaperone-like function for the proper and stable folding of KbaY. When expressed alone, KbaZ does not show any aldolase activity. Ref.4
Pathway	Carbohydrate metabolism; D-tagatose 6-phosphate degradation; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-tagatose 6-phosphate: step 2/2. HAMAP-Rule MF_01295
Subunit structure	Forms a complex with KbaY.
Miscellaneous	In contrast to E.coli strains C and EC3132, K-12 strains can not grow on N-acetylgalactosamine and D-galactosamine, because they carry a deletion and thus lack active PTS systems specific for these compounds. Therefore, KbaYZ in K-12 strains is not involved in the degradation of these compounds. HAMAP-Rule MF_01295
Sequence similarities	Belongs to the GatZ/KbaZ family. KbaZ subfamily.
Caution	Was originally (Ref.3) thought to be a tagatose 6-phosphate kinase.

Ontologies

Keywords
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-tagatose 6-phosphate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway galactitol metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	catalytic activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	D-tagatose-1,6-bisphosphate aldolase subunit KbaZ HAMAP-Rule MF_01295		PRO_0000064493

Sequences

	Sequence		Length	Mass (Da)
	P0C8K0 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: FC97956E662EA939	FASTA	426	47,192
	10 20 30 40 50 60 MKHLTEMVRQ HKAGKTNGIY AVCSAHPLVL EAAIRYASAN QTPLLIEATS NQVDQFGGYT 70 80 90 100 110 120 GMTPADFRGF VCQLADSLNF PQDALILGGD HLGPNRWQNL PAAQAMANAD DLIKSYVAAG 130 140 150 160 170 180 FKKIHLDCSM SCQDDPIPLT DDIVAERAAR LAKVAEETCL EHFGEADLEY VIGTEVPVPG 190 200 210 220 230 240 GAHETLSELA VTTPDAARAT LEAHRHAFEK QGLNAIWPRI IALVVQPGVE FDHTNVIDYQ 250 260 270 280 290 300 PAKASALSQM VENYETLIFE AHSTDYQTPQ SLRQLVIDHF AILKVGPALT FALREALFSL 310 320 330 340 350 360 AAIEEELVPA KACSGLRQVL EDVMLDRPEY WQSHYHGDGN ARRLARGYSY SDRVRYYWPD 370 380 390 400 410 420 SQIDDAFAHL VRNLADSPIP LPLISQYLPL QYVKVRSGEL QPTPRELIIN HIQDILAQYH TACEGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr. Microbiology 142:231-250(1996) [PubMed] [Europe PMC] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
[4]	"Two class II D-tagatose-bisphosphate aldolases from enteric bacteria." Brinkkoetter A., Shakeri-Garakani A., Lengeler J.W. Arch. Microbiol. 177:410-419(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A TAGBP ALDOLASE COMPONENT, COMPLEX WITH KBAY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA57935.1. U00096 Genomic DNA. Translation: AAC76166.1. AP009048 Genomic DNA. Translation: BAE77179.1.
PIR	H65102.
RefSeq	NP_417601.1. NC_000913.2. YP_491320.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0C8K0.
SMR	P0C8K0. Positions 4-423.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0C8K0. 7 interactions.
STRING	511145.b3132.
Protocols and materials databases
DNASU	947637.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76166; AAC76166; b3132. BAE77179; BAE77179; BAE77179.
GeneID	12934380. 947637.
KEGG	ecj:Y75_p3054. eco:b3132.
PATRIC	32121680. VBIEscCol129921_3226.
Organism-specific databases
EchoBASE	EB2616.
EcoGene	EG12763. kbaZ.
Phylogenomic databases
HOGENOM	HOG000263918.
KO	K16371.
OMA	QRHFSYS.
ProtClustDB	PRK15458.
Enzyme and pathway databases
BioCyc	EcoCyc:G7631-MONOMER. MetaCyc:G7631-MONOMER.
UniPathway	UPA00704; UER00716.
Gene expression databases
Genevestigator	P0C8K0.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01295. Tagatose_aldol_KbaZ.
InterPro	IPR013785. Aldolase_TIM. IPR012062. TagBP_ald_GatZ/KbaZ. IPR023435. TagBP_ald_KbaZ. [Graphical view]
Pfam	PF08013. Tagatose_6_P_K. 1 hit. [Graphical view]
PIRSF	PIRSF009264. TagBP_ald_AgaZ. 1 hit.
TIGRFAMs	TIGR02810. agaZ_gatZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KBAZ_ECOLI

Accession

Primary (citable) accession number: P0C8K0
Secondary accession number(s): P42903, Q2M977

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program