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Protein

D-tagatose-1,6-bisphosphate aldolase subunit GatY

Gene

gatY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the tagatose-1,6-bisphosphate aldolase GatYZ, which catalyzes the reversible aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6-bisphosphate (TBP). Requires GatZ subunit for full activity and stability. Is involved in the catabolism of galactitol.2 Publications

Catalytic activityi

D-tagatose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Kineticsi

  1. KM=1.0 mM for tagatose-1,6-bisphosphate (when expressed alone)1 Publication
  2. KM=0.3 mM for tagatose-1,6-bisphosphate (when expressed with GatZ)1 Publication

    Pathwayi: D-tagatose 6-phosphate degradation

    This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-tagatose 6-phosphate.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. D-tagatose-1,6-bisphosphate aldolase subunit GatZ (gatZ), D-tagatose-1,6-bisphosphate aldolase subunit GatY (gatY), D-tagatose-1,6-bisphosphate aldolase subunit KbaY (kbaY), D-tagatose-1,6-bisphosphate aldolase subunit KbaZ (kbaZ)
    This subpathway is part of the pathway D-tagatose 6-phosphate degradation, which is itself part of Carbohydrate metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-tagatose 6-phosphate, the pathway D-tagatose 6-phosphate degradation and in Carbohydrate metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei82Proton donorBy similarity1
    Metal bindingi83Zinc; catalyticBy similarity1
    Metal bindingi180Zinc; catalyticBy similarity1
    Binding sitei181Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
    Metal bindingi208Zinc; catalyticBy similarity1

    GO - Molecular functioni

    • tagatose-bisphosphate aldolase activity Source: EcoCyc
    • zinc ion binding Source: InterPro

    GO - Biological processi

    Keywordsi

    Molecular functionLyase
    Biological processGalactitol metabolism
    LigandMetal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:TAGAALDOL2-MONOMER
    MetaCyc:TAGAALDOL2-MONOMER
    SABIO-RKiP0C8J6
    UniPathwayiUPA00704; UER00716

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-tagatose-1,6-bisphosphate aldolase subunit GatY (EC:4.1.2.40)
    Short name:
    TBPA
    Short name:
    TagBP aldolase
    Alternative name(s):
    D-tagatose-bisphosphate aldolase class II
    Tagatose-bisphosphate aldolase
    Gene namesi
    Name:gatY
    Synonyms:yegF
    Ordered Locus Names:b2096, JW5343
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12419 gatY

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001787661 – 284D-tagatose-1,6-bisphosphate aldolase subunit GatYAdd BLAST284

    Proteomic databases

    PaxDbiP0C8J6
    PRIDEiP0C8J6

    Expressioni

    Inductioni

    Constitutively expressed. Up-regulated under low pH conditions.2 Publications

    Interactioni

    Subunit structurei

    Forms a complex with GatZ.

    Protein-protein interaction databases

    BioGridi4260432, 19 interactors
    DIPiDIP-48239N
    IntActiP0C8J6, 14 interactors
    STRINGi316385.ECDH10B_2249

    Structurei

    3D structure databases

    ProteinModelPortaliP0C8J6
    SMRiP0C8J6
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni209 – 211Dihydroxyacetone phosphate bindingBy similarity3
    Regioni230 – 233Dihydroxyacetone phosphate bindingBy similarity4

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105D2N Bacteria
    COG0191 LUCA
    HOGENOMiHOG000227793
    InParanoidiP0C8J6
    KOiK08302
    OMAiGENFLRH
    PhylomeDBiP0C8J6

    Family and domain databases

    CDDicd00947 TBP_aldolase_IIB, 1 hit
    Gene3Di3.20.20.70, 1 hit
    HAMAPiMF_01294 TagBP_aldolase_GatY, 1 hit
    InterProiView protein in InterPro
    IPR013785 Aldolase_TIM
    IPR000771 FBA_II
    IPR011288 TagBP_ald_KbaY/GatY
    IPR023955 TagBP_aldolase_GatY
    PfamiView protein in Pfam
    PF01116 F_bP_aldolase, 1 hit
    PIRSFiPIRSF001359 F_bP_aldolase_II, 1 hit
    TIGRFAMsiTIGR00167 cbbA, 1 hit
    TIGR01858 tag_bisphos_ald, 1 hit
    PROSITEiView protein in PROSITE
    PS00602 ALDOLASE_CLASS_II_1, 1 hit
    PS00806 ALDOLASE_CLASS_II_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0C8J6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MYVVSTKQML NNAQRGGYAV PAFNIHNLET MQVVVETAAN LHAPVIIAGT
    60 70 80 90 100
    PGTFTHAGTE NLLALVSAMA KQYHHPLAIH LDHHTKFDDI AQKVRSGVRS
    110 120 130 140 150
    VMIDASHLPF AQNISRVKEV VDFCHRFDVS VEAELGQLGG QEDDVQVNEA
    160 170 180 190 200
    DALYTNPAQA REFAEATGID SLAVAIGTAH GMYASAPALD FSRLENIRQW
    210 220 230 240 250
    VNLPLVLHGA SGLSTKDIQQ TIKLGICKIN VATELKNAFS QALKNYLTEH
    260 270 280
    PEATDPRDYL QSAKSAMRDV VSKVIADCGC EGRA
    Length:284
    Mass (Da):30,812
    Last modified:November 25, 2008 - v1
    Checksum:iD414AA3921BBFE2B
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA Translation: AAC75157.2
    AP009048 Genomic DNA Translation: BAA15966.2
    PIRiS55901
    RefSeqiNP_416599.2, NC_000913.3
    WP_001307281.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC75157; AAC75157; b2096
    BAA15966; BAA15966; BAA15966
    GeneIDi946636
    KEGGiecj:JW5343
    eco:b2096
    PATRICifig|1411691.4.peg.151

    Similar proteinsi

    Entry informationi

    Entry nameiGATY_ECOLI
    AccessioniPrimary (citable) accession number: P0C8J6
    Secondary accession number(s): P37192, P76415
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: November 25, 2008
    Last modified: March 28, 2018
    This is version 79 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health