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P0C8I5

- KITH_ASFP4

UniProt

P0C8I5 - KITH_ASFP4

Protein

Thymidine kinase

Gene

Pret-062

Organism
African swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) (ASFV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 19 (01 Oct 2014)
      Sequence version 1 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine By similarity.By similarity

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921Proton acceptorSequence Analysis
    Binding sitei121 – 1211Substrate; via amide nitrogenBy similarity
    Metal bindingi146 – 1461ZincBy similarity
    Metal bindingi149 – 1491ZincBy similarity
    Metal bindingi179 – 1791ZincBy similarity
    Metal bindingi182 – 1821ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi17 – 248ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. thymidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis, Virulence

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Short name:
    TDK
    Gene namesi
    Ordered Locus Names:Pret-062
    OrganismiAfrican swine fever virus (isolate Tick/South Africa/Pretoriuskop Pr4/1996) (ASFV)
    Taxonomic identifieri561443 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
    Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
    Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
    Phacochoerus africanus (Warthog) [TaxID: 41426]
    Potamochoerus larvatus (Bushpig) [TaxID: 273792]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000000859: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 188188Thymidine kinasePRO_0000355224Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP0C8I5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 1705Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0C8I5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIIRKLKPG TISLVLGPMF AGKTTFLIHC IYMLERLEKK VVFIKSTKNT    50
    RDKTIKTHSG IQLRPKQCKI IESTQLSDVG SLTDIHAVVI DEAHFFDDLI 100
    KCRTWADEEK IIILAGLNAS FEQKMFPSIV RIFPYCSWVK YIGRTCMKCN 150
    RHNACFNVRK NADKTLILAG GSELYVTCCN NCLKKYIY 188
    Length:188
    Mass (Da):21,532
    Last modified:November 25, 2008 - v1
    Checksum:iF6352B2E69E73A94
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY261363 Genomic DNA. No translation available.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY261363 Genomic DNA. No translation available.

    3D structure databases

    ProteinModelPortali P0C8I5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "African swine fever virus genomes."
      Kutish G.F., Rock D.L.
      Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiKITH_ASFP4
    AccessioniPrimary (citable) accession number: P0C8I5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 19 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3