P0C8I4 (KITH_ASFM2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
Short name=TDK
EC=2.7.1.21

Gene names

Ordered Locus Names:

Mal-058

Organism

African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV) [Complete proteome]

Taxonomic identifier

10500 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus › African swine fever virus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine. Ref.2
Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis Virulence
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Thymidine kinase		PRO_0000355223
Regions
	Nucleotide binding	17 – 24	8	ATP By similarity
	Region	166 – 170	5	Substrate binding By similarity
Sites
	Active site	92	1	Proton acceptor Potential
	Metal binding	146	1	Zinc By similarity
	Metal binding	149	1	Zinc By similarity
	Metal binding	179	1	Zinc By similarity
	Metal binding	182	1	Zinc By similarity
	Binding site	121	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C8I4 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 0047D0121BE88938	FASTA	188	21,483
	10 20 30 40 50 60 MNIIRKLKPG TISLVLGPMF AGKTTFLIHC IYMLERLEKK VVFIKSTKNT RDKTIKTHSG 70 80 90 100 110 120 IQLQSKQCEI IESTQLSDVG SLTDIHAVVI DEAHFFDDLI KCRAWADEEK IIILAGLNAS 130 140 150 160 170 180 FEQKMFQPIV HIFPYCSWIK YIGRTCMKCN RHNACFNVRK NADKTLILAG GSELYVTCCN NCLKKQMY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"African swine fever virus genomes." Kutish G.F., Rock D.L. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"The African swine fever virus thymidine kinase gene is required for efficient replication in swine macrophages and for virulence in swine." Moore D.M., Zsak L., Neilan J.G., Lu Z., Rock D.L. J. Virol. 72:10310-10315(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY261361 Genomic DNA. No translation available.
3D structure databases
ProteinModelPortal	P0C8I4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_ASFM2

Accession

Primary (citable) accession number: P0C8I4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	October 3, 2012
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program