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Protein

Thymidine kinase

Gene

Mal-058

Organism
African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine.1 Publication

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92Proton acceptorSequence analysis1
Binding sitei121Substrate; via amide nitrogenBy similarity1
Metal bindingi146ZincBy similarity1
Metal bindingi149ZincBy similarity1
Metal bindingi179ZincBy similarity1
Metal bindingi182ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 24ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processDNA synthesis, Virulence
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Short name:
TDK
Gene namesi
Ordered Locus Names:Mal-058
OrganismiAfrican swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV)
Taxonomic identifieri10500 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000000860 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003552231 – 188Thymidine kinaseAdd BLAST188

Structurei

3D structure databases

ProteinModelPortaliP0C8I4.
SMRiP0C8I4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 170Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

OrthoDBiVOG090000XT.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiView protein in Pfam
PF00265. TK. 1 hit.
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00603. TK_CELLULAR_TYPE. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C8I4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIIRKLKPG TISLVLGPMF AGKTTFLIHC IYMLERLEKK VVFIKSTKNT
60 70 80 90 100
RDKTIKTHSG IQLQSKQCEI IESTQLSDVG SLTDIHAVVI DEAHFFDDLI
110 120 130 140 150
KCRAWADEEK IIILAGLNAS FEQKMFQPIV HIFPYCSWIK YIGRTCMKCN
160 170 180
RHNACFNVRK NADKTLILAG GSELYVTCCN NCLKKQMY
Length:188
Mass (Da):21,483
Last modified:November 25, 2008 - v1
Checksum:i0047D0121BE88938
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY261361 Genomic DNA. No translation available.

Similar proteinsi

Entry informationi

Entry nameiKITH_ASFM2
AccessioniPrimary (citable) accession number: P0C8I4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: August 30, 2017
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families