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P0C8I4 (KITH_ASFM2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine. Ref.2

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sequence similarities

Belongs to the thymidine kinase family.

Ontologies

Keywords
   Biological processDNA synthesis
Virulence
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

pathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

thymidine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 188188Thymidine kinase
PRO_0000355223

Regions

Nucleotide binding17 – 248ATP By similarity
Region166 – 1705Substrate binding By similarity

Sites

Active site921Proton acceptor Potential
Metal binding1461Zinc By similarity
Metal binding1491Zinc By similarity
Metal binding1791Zinc By similarity
Metal binding1821Zinc By similarity
Binding site1211Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P0C8I4 [UniParc].

Last modified November 25, 2008. Version 1.
Checksum: 0047D0121BE88938

FASTA18821,483
        10         20         30         40         50         60 
MNIIRKLKPG TISLVLGPMF AGKTTFLIHC IYMLERLEKK VVFIKSTKNT RDKTIKTHSG 

        70         80         90        100        110        120 
IQLQSKQCEI IESTQLSDVG SLTDIHAVVI DEAHFFDDLI KCRAWADEEK IIILAGLNAS 

       130        140        150        160        170        180 
FEQKMFQPIV HIFPYCSWIK YIGRTCMKCN RHNACFNVRK NADKTLILAG GSELYVTCCN 


NCLKKQMY 

« Hide

References

« Hide 'large scale' references
[1]"African swine fever virus genomes."
Kutish G.F., Rock D.L.
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"The African swine fever virus thymidine kinase gene is required for efficient replication in swine macrophages and for virulence in swine."
Moore D.M., Zsak L., Neilan J.G., Lu Z., Rock D.L.
J. Virol. 72:10310-10315(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY261361 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortalP0C8I4.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKITH_ASFM2
AccessionPrimary (citable) accession number: P0C8I4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: April 16, 2014
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families