Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Thymidine kinase

Gene

Ken-062

Organism
African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine (By similarity).By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92Proton acceptorSequence analysis1
Binding sitei121Substrate; via amide nitrogenBy similarity1
Metal bindingi146ZincBy similarity1
Metal bindingi149ZincBy similarity1
Metal bindingi179ZincBy similarity1
Metal bindingi182ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 24ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processDNA synthesis, Virulence
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Short name:
TDK
Gene namesi
Ordered Locus Names:Ken-062
OrganismiAfrican swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV)
Taxonomic identifieri561445 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
Phacochoerus africanus (Warthog) [TaxID: 41426]
Potamochoerus larvatus (Bushpig) [TaxID: 273792]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000000861 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003552221 – 185Thymidine kinaseAdd BLAST185

Structurei

3D structure databases

ProteinModelPortaliP0C8I3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 170Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

OrthoDBiVOG090900MG.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiView protein in Pfam
PF00265. TK. 1 hit.
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00603. TK_CELLULAR_TYPE. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C8I3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLIRKLKPG TISLVLGPMF AGKTTFLIHC INMLERLEKK VVFIKSTKNT
60 70 80 90 100
RDKTIQTHSG IQLRPNQCKI IESAQLSDVG SLTDTHAVVI DEAHFFDDLI
110 120 130 140 150
RCRTWADEKK IIILAGLNAS FEQKMFQPIV RIFPYCNWVK YIGRTCMKCN
160 170 180
RHNACFNVRK NADKTLILAG GSELYITCCN NCLKK
Length:185
Mass (Da):21,069
Last modified:November 25, 2008 - v1
Checksum:i46CF25F8C4748674
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY261360 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY261360 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortaliP0C8I3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG090900MG.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiView protein in Pfam
PF00265. TK. 1 hit.
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00603. TK_CELLULAR_TYPE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_ASFK5
AccessioniPrimary (citable) accession number: P0C8I3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: February 15, 2017
This is version 19 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.