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P0C8I3

- KITH_ASFK5

UniProt

P0C8I3 - KITH_ASFK5

Protein

Thymidine kinase

Gene

Ken-062

Organism
African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 17 (01 Oct 2014)
      Sequence version 1 (25 Nov 2008)
      Previous versions | rss
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    Functioni

    Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine By similarity.By similarity

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921Proton acceptorSequence Analysis
    Binding sitei121 – 1211Substrate; via amide nitrogenBy similarity
    Metal bindingi146 – 1461ZincBy similarity
    Metal bindingi149 – 1491ZincBy similarity
    Metal bindingi179 – 1791ZincBy similarity
    Metal bindingi182 – 1821ZincBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi17 – 248ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. thymidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis, Virulence

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Short name:
    TDK
    Gene namesi
    Ordered Locus Names:Ken-062
    OrganismiAfrican swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV)
    Taxonomic identifieri561445 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
    Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
    Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
    Phacochoerus africanus (Warthog) [TaxID: 41426]
    Potamochoerus larvatus (Bushpig) [TaxID: 273792]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000000861: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 185185Thymidine kinasePRO_0000355222Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP0C8I3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 1705Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the thymidine kinase family.Curated

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0C8I3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLIRKLKPG TISLVLGPMF AGKTTFLIHC INMLERLEKK VVFIKSTKNT    50
    RDKTIQTHSG IQLRPNQCKI IESAQLSDVG SLTDTHAVVI DEAHFFDDLI 100
    RCRTWADEKK IIILAGLNAS FEQKMFQPIV RIFPYCNWVK YIGRTCMKCN 150
    RHNACFNVRK NADKTLILAG GSELYITCCN NCLKK 185
    Length:185
    Mass (Da):21,069
    Last modified:November 25, 2008 - v1
    Checksum:i46CF25F8C4748674
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY261360 Genomic DNA. No translation available.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY261360 Genomic DNA. No translation available.

    3D structure databases

    ProteinModelPortali P0C8I3.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "African swine fever virus genomes."
      Kutish G.F., Rock D.L.
      Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiKITH_ASFK5
    AccessioniPrimary (citable) accession number: P0C8I3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: November 25, 2008
    Last modified: October 1, 2014
    This is version 17 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3